ZnO nanoparticle/nanorod-based label-free electrochemical immunoassay for rapid detection of MMP-9 biomarker

Shabani, E ; Sharif University of Technology | 2020

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  1. Type of Document: Article
  2. DOI: 10.1016/j.bej.2020.107772
  3. Publisher: Elsevier B.V , 2020
  4. Abstract:
  5. A label-free electrochemical biosensor was developed for the rapid detection of the matrix metalloproteinase 9 (MMP-9) biomarker on the basis of antibody immobilizing on the zinc oxide (ZnO) nanoparticle and ZnO nanorod electrodes. The charge transfer resistance (Rct) of the electrodes was used as the indicator for MMP-9 concentration, which was obtained through cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The ZnO nanorod-based biosensor exhibited linear behavior in the MMP-9 concentration range of 1–1000 ng/ml, which is a wider range than the available concentration ranges for most of the conventional methods. The biosensor sensitivity was 32.5 μA/(decade × cm2) with a detection time of only 35 min. Moreover, the results from the developed biosensor were comparable with those measured with a commercial enzyme-linked immunosorbent assay (ELISA) for real samples showing less than 8% difference. The presented MMP-9 biosensor is potentially applicable to the analysis of biological samples, especially in point-of-care (POC) diagnosis or urgent cases analysis, considering its simple fabrication, similar accuracy, shorter detection time, and ease of usage compared with traditional methods such as ELISA. © 2020
  6. Keywords:
  7. Electrochemical immunosensor ; Etection ; Label-free ; MMP-9 ; Rapid ; ZnO nanoparticles ; ZnO nanorods ; Bioassay ; Biomarkers ; Biosensors ; Charge transfer ; Chemical detection ; Cyclic voltammetry ; Electrochemical electrodes ; Electrochemical impedance spectroscopy ; II-VI semiconductors ; Nanoparticles ; Nanorods ; Oxide minerals ; Zinc oxide ; Biological samples ; Charge transfer resistance ; Commercial enzymes ; Concentration ranges ; Conventional methods ; Electrochemical biosensor ; Electrochemical immunoassays ; Matrix metalloproteinase-9 ; Gelatinase B ; Immobilized antibody ; Nanorod ; Zinc oxide nanoparticle ; Controlled study ; Electrochemical analysis ; Enzyme linked immunosorbent assay ; Immunoassay ; Impedance spectroscopy ; Intermethod comparison ; Measurement accuracy ; Point of care testing ; Priority journal ; Sensitivity and specificity ; X ray diffraction
  8. Source: Biochemical Engineering Journal ; Volume 164 , 2020
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S1369703X20303260