Design and Fabrication of Three Dimensional Scaffold Using Bio-Printing Technique for Cartilage Tissue Engineering

Mohsenifard, Sadaf | 2021

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 53960 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Mashayekhan, Shohreh; Hassanzadeh, Zabiollah
  7. Abstract:
  8. Herein, we successfully prepared two types of hydrogels, alginate-beta-cyclodextrin based hydrogel and alginate without beta-cyclodextrin hydrogel in order to use in cartilage tissue engineering. At first, sodium alginate was modified with beta-cyclodextrin ring due to incorporate the kartogenin (KGN) differentiating drug in the hydrogel structure. For confirming the correctness of the modification reactions, H-NMR spectroscopy was used. In the presence of phenolized-ECM, alginic acid, modified alginate with beta cyclodextrin, HRP, H2O2, calcium carbonate, and glucono-delta-lactone (GDL), ECM-alginate-IPN and ECM-modified-alginate-IPN hydrogels were synthesized. Then, the KGN was loaded on two types of hydrogels. The results show that the KGN release from ECM-modified-alginate-IPN hydrogel in comparison to ECM-alginate-IPN has a slower KGN release. The drug release in the modified hydrogel reduced from 85% to 65% in 200 hours. Then, different properties of ECM-modified alginate IPN hydrogel properties including stiffness, swelling, degradation and GAG and collagen II content determinations with mesenchymal stem cells were investigated. After that, for modification of mechanical properties, reinforcing soft hydrogels with highly organized, high-porosity microfiber networks that are 3D-printed was used and the un-crosslinked hydrogel solution was casted in these printed micro fibers and then crosslinked with calcium chloride 10% for further crosslinking. At the end of the study, KGN release test for the modified hydrogel, compression mechanical test for the combination of hydrogel and frameworks. The results illustrated that the synthesized hydrogel with modified alginate is promising for cartilage tissue regeneration
  9. Keywords:
  10. Interpenetrating Polymer Network ; Cartilage Tissue Engineering ; Three Dimensional Printer ; Extracellular Matrix ; Kartogenin ; Three Dimensional Scaffolds

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