Characterization of a moderate thermophilic Nocardia species able to grow on polycyclic aromatic hydrocarbons

Zeinali, M ; Sharif University of Technology | 2007

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  1. Type of Document: Article
  2. DOI: 10.1111/j.1472-765X.2007.02241.x
  3. Publisher: 2007
  4. Abstract:
  5. Aims: Our goal was the characterization of a new moderate thermophilic polycyclic aromatic hydrocarbon (PAH)-utilizing Nocardia strain. Methods and Results: A thermophilic bacterium, strain TSH1, was isolated from a contaminated soil. The macroscopic and microscopic features fit well with the description of Nocardia species. The results of 16S rRNA gene analysis showed 100% match to the type strain of N. otitidiscaviarum DSM 43242T. Strain TSH1 showed the same mycolic acid pattern as the type strain of N. otitidiscaviarum but its fatty acid profile did not permit identification to the species level. The carbon utilization profile of strain TSH1 was different from N. otitidiscaviarum. The results of hydrophobicity measurements showed that PAHs-grown cells were significantly more hydrophobic than LB-grown cells. Furthermore, biosurfactant production was detected during bacterial growth on different culture media. Conclusions: Strain TSH1 is capable of growing on a range of PAHs. When grown in PAHs-supplemented media, strain TSH1 showed a high affinity for the organic phase, suggesting that it can develop a hydrophobic surface. Significance and Impact of the Study: High cell surface hydrophobicity and capability of strain TSH1 to degrade different PAHs at 50°C may make it an ideal candidate to treat PAH-contaminated desert soils. © 2007 The Authors
  6. Keywords:
  7. Biosurfactant ; Mycolic acid ; Polycyclic aromatic hydrocarbon ; RNA 16S ; Bacteria ; Genes ; Polycyclic aromatic hydrocarbons ; Strain ; Fatty acid ; Growth rate ; PAH ; RNA ; Soil pollution ; Thermophilic bacterium ; Bacterial growth ; Bacterial strain ; Bacterium isolate ; Carbon utilization ; Controlled study ; Culture medium ; Genetic analysis ; Hydrophobicity ; Microscope ; Nonhuman ; Bacterial Typing Techniques ; Base Composition ; DNA, Bacterial ; DNA, Ribosomal ; Environmental Pollution ; Fatty Acids ; Genes, rRNA ; Phylogeny ; RNA, Bacterial ; RNA, Ribosomal, 16S ; Sequence Analysis, DNA ; Sequence Homology, Nucleic Acid ; Soil Microbiology ; Surface-Active Agents ; Bacteria (microorganisms) ; Nocardia ; Nocardia otitidiscaviarum
  8. Source: Letters in Applied Microbiology ; Volume 45, Issue 6 , December , 2007 , Pages 622-628 ; 02668254 (ISSN)
  9. URL: https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.2007.02241.x