Design and Optimization of Microfluidic-Based Systems for Bioimpedance Spectroscopy in Cell Sorting

Esmaeili, Fatemeh | 2021

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 54146 (05)
  4. University: Sharif University of Technology
  5. Department: Electrical Engineering
  6. Advisor(s): Fardmanesh, Mehdi; Fotowat Ahmady, Ali
  7. Abstract:
  8. Microfluidics is an emerging technology that has attracted lots of attention in recent years due to its remarkable capabilities. This technology that operates fluids in micron dimensions, has led to the emergence of lab-on-a-chip platforms that have the ability to perform one or more laboratory operations on a small scale. One of the most important applications of lab-on-a-chip devices is cell sorting which is done in various methods. In this study, we intend to provide a simple and practical solution for cell sorting by designing a comprehensive and optimized system. In this regard, we design a new generation of microfluidic devices, known as digital microfluidics, which has the ability to manipulate droplets and can be used for sample preparation. In the next step, we design a microchannel that is suitable for separating cells using dielectrophoresis. Dielectrophoresis is an electrostatic force caused by the polarization of the particle and the sorounding medium in a nonuniform electric field. The magnitude and orientation of this force is depended on the characteristics and polarization of the particles. To utilize dielectrophoresis in a microfluidic channel, we design three dimensional electrodes that can not only separate the particles but also are capable of focusing them into a line at the center. Thus, the overall structure can be much simpler by using only one pump. Finally, we measure the electrical conductivity of the samples using a system designed for bioimpedance spectroscopy
  9. Keywords:
  10. Microfluidic System ; Dielectrophoresis Technique ; Cell Separation ; Microfluidic Devices ; Digital Microfluidics ; Channel-Based Microfluidics ; Bioimpedance Spectroscopy

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