Metabolic Engineering of Yeast Saccharomyces Cerevisiae for Production of Fatty Acids and Its Derivatives

Homayoonfar, Mohammad | 2021

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 54398 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Roosta Azad, Reza; Sardari, Soroush
  7. Abstract:
  8. With the increasing emphasis on production of various chemicals by biotechnology industry, the establishment of optimal cell factories for the maximum production of a specific chemical product will be a serious challenge in large-scale production in this industry. Metabolic engineering is a branch of biochemical engineering that has been developed to achieve this and the approaches in it seek to address this challenge. In this study, focusing on increasing production of fatty acids by yeast cells of Saccharomyces cerevisiae, and using a combination of computational and laboratory methods to present a methodology for the optimal design of culture media. Thus, using the constraint-based modeling as well as modeling based on graph theory, effective components achieving the goal have been selected. Then, by applying certain amounts of these components to the basal culture medium of yeast cells, change in fatty acid content were investigated using the highthroughput method of Nile red adsorption. The combined application of the above computational methods for the purpose of optimal design of the culture media according to this methodology, has been proposed for the first time in the literature. The results showed an increase in total fatty acids while applying a growth enhancer, which could confirm the hypothesis that the components that enhance cell growth necessarily enhance growth-associated products
  9. Keywords:
  10. Saccharomyces Cerevisiae ; Constraint-Based Modeling ; Metabolic Engineering ; Metabolic Graphs ; Acid Production ; Fatty Acids ; Fission Yeast

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