Experimental and Numerical Study of The Production of Alginate Microgels and Cancer Spheroids by Droplet-Based Microfluidic

Rezaeian, Masoud | 2021

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 54410 (08)
  4. University: Sharif University of Technology
  5. Department: Mechanical Engineering
  6. Advisor(s): Shamloo, Amir; Kazemzadeh Hannani, Siamak
  7. Abstract:
  8. Significant advances in biotechnology have led to the emergence of a cost-effective way with less ethical issues to study disease, organ functions, tumors, and their response to drugs besides studying on animals. Microfluidic devices and organ on a chip (tumor on a chip) were introduced to remove those obstacles. Organ on a chip is a powerful tool for studying different types of tissues and simulating diseases, especially cancers, for biological and medical applications. Organ (tumor) on a chip is considered as a smaller scale of the real organ or tumor and it causes to the real-time study of tissues and their functions more accurately. In this study, to fabricate a droplet-based microfluidic for cell encapsulation and aggregation purposes, microfluidic channels were designed and simulated. Then, the designed microfluidic device was fabricated by photo and soft lithography, and optimized flow rates were numerically obtained by simulating the droplet formation of sodium alginate solution as a non-Newtonian fluid. In another part of this study, HT-29 cancer cells were cultured and encapsulated in the microfluidic device to simulate 3D in-vivo conditions.The results showed that not only droplet-based microfluidic devices is a good option for culturing cells in a 3D microenvironment, but also it causes cell aggregation within monodispersed droplets to perform other tests such as drug screening. finally, the viability of encapsulated cells in alginate microgels was investigated and it was found that the introduced method for producing alginate microgels has suitable biocompatibility for biological applications
  9. Keywords:
  10. Droplet Formation ; Three Dimentional Cell Culture ; Cell Culture ; Microfluidics Droplet Generation ; Droplet-Based Microfluidics ; Tumor Microenvironment ; Alginate Microgel

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