Serological assays and host antibody detection in coronavirus-related disease diagnosis

Dowlatshahi, S ; Sharif University of Technology | 2021

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  1. Type of Document: Article
  2. DOI: 10.1007/s00705-020-04874-2
  3. Publisher: Springer , 2021
  4. Abstract:
  5. Coronaviruses (CoV) are a family of viral pathogens that infect both birds and mammals, including humans. Seven human coronaviruses (HCoV) have been recognized so far. HCoV-229E, -OC43, -NL63, and -HKU1 account for one-third of common colds with mild symptoms. The other three members are severe acute respiratory syndrome (SARS)-CoV, Middle East respiratory syndrome (MERS)-CoV, and SARS-CoV-2. These viruses are responsible for SARS, MERS, and CoV disease 2019 (COVID-19), respectively. A variety of diagnostic techniques, including chest X-rays, computer tomography (CT) scans, analysis of viral nucleic acids, proteins, or whole virions, and host antibody detection using serological assays have been developed for the detection of these viruses. In this review, we discuss conventional serological tests, such as enzyme-linked immunosorbent assay (ELISA), western blot (WB), immunofluorescence assay (IFA), lateral flow immunoassay (LFIA), and chemiluminescence immunoassay (CLIA), as well as biosensor-based assays that have been developed for diagnosing HCoV-associated diseases since 2003, with an in-depth focus on COVID-19. © 2021, Springer-Verlag GmbH Austria, part of Springer Nature
  6. Keywords:
  7. Virus antibody ; Blood ; Enzyme linked immunosorbent assay ; Fluorescent antibody technique ; Genetic procedures ; Human ; Immunology ; Luminescence ; Middle East respiratory syndrome coronavirus ; procedures ; SARS coronavirus ; Severe acute respiratory syndrome ; Virology ; Western blotting ; Antibodies, Viral ; Biosensing Techniques ; Blotting, Western ; COVID-19 ; COVID-19 Serological Testing ; Enzyme-Linked Immunosorbent Assay ; Humans ; Luminescent Measurements ; SARS Virus ; SARS-CoV-2
  8. Source: Archives of Virology ; Volume 166, Issue 3 , 2021 , Pages 715-731 ; 03048608 (ISSN)
  9. URL: https://pubmed.ncbi.nlm.nih.gov/33492524