Expression analysis of protein inhibitor of activated stat in inflammatory demyelinating polyradiculoneuropathy

Ghafouri Fard, S ; Sharif University of Technology | 2021

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  1. Type of Document: Article
  2. DOI: 10.3389/fimmu.2021.659038
  3. Publisher: Frontiers Media S.A , 2021
  4. Abstract:
  5. Protein inhibitors of activated STAT (PIAS) are involved in the regulation of the JAK/STAT signaling pathway and have interactions with NF-κB, p73 and p53. These proteins regulate immune responses; therefore dysregulation in their expression leads to several immune-mediated disorders. In the present study, we examined expression of PIAS1-4 in peripheral blood of patients with acute/chronic inflammatory demyelinating polyradiculoneuropathy (AIDP/CIDP) compared with healthy subjects. We demonstrated down-regulation of all PIAS genes in both AIDP and CIDP cases compared with controls. Similarly, comparisons in gender-based groups revealed down-regulation of these gene0s in patients of each gender compared with gender-matched controls. There was no significant difference in expression of PIAS genes between AIDP and CIDP cases. Based on the area under the receiver operating characteristic curves, PIAS1-4 genes could distinguish between inflammatory demyelinating polyradiculoneuropathy and healthy status with accuracy values of 0.87, 0.87, 0.79 and 0.80, respectively. In differentiation between AIDP cases and healthy controls, these values were 0.92, 0.92, 0.83 and 0.86, respectively. Finally, PIAS1-4 genes could discriminate CIDP from healthy status with accuracy values of 0.82, 0.83, 0.75 and 0.75, respectively. The current study underscores the role of PIAS genes in the pathogenesis of inflammatory demyelinating polyradiculoneuropathy and their potential usage as biomarkers. © Copyright © 2021 Ghafouri-Fard, Hussen, Nicknafs, Nazer, Sayad and Taheri
  6. Keywords:
  7. Protein inhibitor of activated STAT ; Biological marker ; Acute inflammatory demyelinating polyneuropathy ; CD4+ T lymphocyte ; Chronic inflammatory demyelinating polyradiculoneuropathy ; Controlled study ; Diagnostic test accuracy study ; Down regulation ; Gene expression assay ; Genetic marker ; Human ; Machine learning ; Major clinical study ; Pathogenesis ; Protein expression ; Real time polymerase chain reaction ; Receiver operating characteristic ; Regulatory T lymphocyte ; RNA extraction ; Sensitivity and specificity ; Disease predisposition ; Genetics ; Guillain Barre syndrome ; Metabolism ; Polyradiculoneuropathy ; Adult ; Aged ; Bayes Theorem ; Biomarkers ; Disease Management ; Disease Susceptibility ; Female ; Gene Expression Regulation ; Guillain-Barre Syndrome ; Humans ; Male ; Middle Aged ; Multigene Family ; Polyradiculoneuropathy, Chronic Inflammatory Demyelinating ; Prognosis ; Protein Inhibitors of Activated STAT ; ROC Curve
  8. Source: Frontiers in Immunology ; Volume 12 , 2021 ; 16643224 (ISSN)
  9. URL: https://www.frontiersin.org/articles/10.3389/fimmu.2021.659038/full