Laboratory detection methods for the human coronaviruses

Shabani, E ; Sharif University of Technology | 2021

443 Viewed
  1. Type of Document: Article
  2. DOI: 10.1007/s10096-020-04001-8
  3. Publisher: Springer Science and Business Media Deutschland GmbH , 2021
  4. Abstract:
  5. Coronaviruses are a group of envelop viruses which lead to diseases in birds and mammals as well as human. Seven coronaviruses have been discovered in humans that can cause mild to lethal respiratory tract infections. HCoV-229E, HCoV-OC43, HCoV-NL63, and HCoV-HKU1 are the low-risk members of this family and the reason for some common colds. Besides, SARS-CoV, MERS-CoV, and newly identified SARS-CoV-2, which is also known as 2019-nCoV, are the more dangerous viruses. Due to the rapid spread of this novel coronavirus and its related disease, COVID-19, a reliable, simple, fast, and low-cost detection method is necessary for patient diagnosis and tracking worldwide. Human coronaviruses detection methods were classified and presented in this article. The laboratory detection techniques include RT-PCR, RT-LAMP, electrochemical and optical biosensors for RNA detection, and whole virus or viral proteins detection assays. © 2020, Springer-Verlag GmbH Germany, part of Springer Nature
  6. Keywords:
  7. Double stranded DNA ; Viral protein ; Virus RNA ; Virus RNA ; Common cold ; Coronaviridae ; Coronavirus disease 2019 ; Gene amplification ; Human coronavirus 229E ; Human coronavirus HKU1 ; Human coronavirus NL63 ; Human coronavirus OC43 ; Laboratory diagnosis ; Mid-turbinate swab ; Middle East respiratory syndrome coronavirus ; Nasopharyngeal swab ; Nonhuman ; Nose smear ; Oropharyngeal swab ; Priority journal ; Respiratory tract infection ; Reverse transcription loop mediated isothermal amplification ; Review ; SARS coronavirus ; Severe acute respiratory syndrome coronavirus 2 ; Sputum smear ; Virus detection ; Diagnosis ; Electrochemical analysis ; Genetic procedures ; Human ; Isolation and purification ; Molecular diagnosis ; Nucleic acid amplification ; Procedures ; Real time polymerase chain reaction ; Reverse transcription polymerase chain reaction ; Biosensing Techniques ; COVID-19 ; COVID-19 Testing ; Electrochemical Techniques ; Humans ; Molecular Diagnostic Techniques ; Nucleic Acid Amplification Techniques ; Real-Time Polymerase Chain Reaction ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Viral ; SARS-CoV-2
  8. Source: European Journal of Clinical Microbiology and Infectious Diseases ; Volume 40, Issue 2 , 2021 , Pages 225-246 ; 09349723 (ISSN)
  9. URL: https://link.springer.com/article/10.1007/s10096-020-04001-8