Fabrication and evaluation of chitosan/gelatin/PVA hydrogel incorporating honey for wound healing applications: An in vitro, in vivo study

Shamloo, A ; Sharif University of Technology | 2021

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  1. Type of Document: Article
  2. DOI: 10.1016/j.ijpharm.2020.120068
  3. Publisher: Elsevier B.V , 2021
  4. Abstract:
  5. In this study, physically cross-linked hydrogels were developed by freezing-thawing method while different concentrations of honey were included into the hydrogels for accelerated wound healing. The hydrogel was composed of chitosan, polyvinyl alcohol (PVA), and gelatin with the ratio of 2:1:1 (v/v), respectively. Further, the effect of honey concentrations on antibacterial properties, and cell behavior was investigated. In vivo studies, including wound healing mechanism using rat model and histological analysis of section tissue samples were performed. The results illustrated that the incorporation of honey in hydrogels increased the ultimate strain of hydrogels approximately two times, while reduced the ultimate tensile strength and elastic modulus of hydrogels. Moreover, the antibacterial activities of samples were increased by increasing the concentration of honey. Regarding MTT assay, as the concentration of honey increased, the cell viability of hydrogels was enhanced until an optimal amount of honey. Further, the integration of honey into the hydrogel matrix results in the maintenance of a well-structured layer of epidermis containing mature collagen and accelerates the rate of wound healing. The 3D Chitosan/PVA/Gelatin hydrogel containing honey with appropriate mechanical, antibacterial activity, and biocompatibility could be a promising approach for wound healing. © 2020 Elsevier B.V
  6. Keywords:
  7. Chitosan ; Collagen ; Hydrogel ; Animal cell ; Animal experiment ; Animal model ; Animal tissue ; Antibacterial activity ; Biocompatibility ; Cell function ; Cell viability ; Controlled study ; Cross linking ; Epidermis ; Freezing ; In vitro study ; In vivo study ; Male ; MTT assay ; Nonhuman ; Priority journal ; Pseudomonas aeruginosa ; Rat ; Staphylococcus aureus ; Tensile strength ; Thawing ; Tissue engineering ; Wound healing ; Young modulus ; Animal ; Animals ; Gelatin ; Honey ; Hydrogels ; Polyvinyl Alcohol ; Rats
  8. Source: International Journal of Pharmaceutics ; Volume 592 , 2021 ; 03785173 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S037851732031053X