A hydrogen-bonded extracellular matrix-mimicking bactericidal hydrogel with radical scavenging and hemostatic function for ph-responsive wound healing acceleration

Ahmadian, Z ; Sharif University of Technology | 2021

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  1. Type of Document: Article
  2. DOI: 10.1002/adhm.202001122
  3. Publisher: Wiley-VCH Verlag , 2021
  4. Abstract:
  5. Generation of reactive oxygen species, delayed blood clotting, prolonged inflammation, bacterial infection, and slow cell proliferation are the main challenges of effective wound repair. Herein, a multifunctional extracellular matrix-mimicking hydrogel is fabricated through abundant hydrogen bonding among the functional groups of gelatin and tannic acid (TA) as a green chemistry approach. The hydrogel shows adjustable physicochemical properties by altering the concentration of TA and it represents high safety features both in vitro and in vivo on fibroblasts, red blood cells, and mice organs. In addition to the merit of facile encapsulation of cell proliferation-inducing hydrophilic drugs, accelerated healing of skin injury is obtained through pH-dependent release of TA and its multifaceted mechanisms as an antibacterial, antioxidant, hemostatic, and anti-inflammatory moiety. The developed gelatin-TA (GelTA) hydrogel also shows an outstanding effect on the formation of extracellular matrix and wound closure in vivo via offered cell adhesion sites in the backbone of gelatin that provide increased re-epithelialization and better collagen deposition. These results suggest that the multifunctional GelTA hydrogel is a promising candidate for the clinical treatment of full-thickness wounds and further development of wound dressing materials that releases active agents in the neutral or slightly basic environment of infected nonhealing wounds. © 2020 Wiley-VCH GmbH
  6. Keywords:
  7. Blood ; Cell adhesion ; Cell culture ; Cell proliferation ; Cells ; Controlled drug delivery ; Green manufacturing ; Hydrogels ; Hydrogen ; Mammals ; Physicochemical properties ; Targeted drug delivery ; Tissue regeneration ; Anti-inflammatories ; Bacterial infections ; Clinical treatments ; Extracellular matrices ; Green chemistry approaches ; Ph-dependent release ; Re-epithelialization ; Wound dressing materials ; Hydrogen bonds ; Collagen ; Gelatin ; Hydrogel ; Tannin ; Antiinfective agent ; Hemostatic agent ; Animal cell ; Animal tissue ; Antibacterial activity ; Antiinflammatory activity ; Antioxidant activity ; Blood clotting ; Cell encapsulation ; Cell viability ; Controlled study ; DPPH radical scavenging assay ; Epithelization ; Fibroblast ; Fourier transform infrared spectroscopy ; Hemostasis ; In vitro study ; In vivo study ; Male ; Mouse ; Nonhuman ; Rat ; Synthesis ; Thermostability ; Tissue engineering ; Water retention ; Wound healing ; X ray diffraction ; Animal ; Hydrogen bond ; PH ; Acceleration ; Animals ; Anti-Bacterial agents ; Extracellular matrix ; Hemostatics ; Hydrogen ; Hydrogen bonding ; Hydrogen-Ion Concentration ; Mice
  8. Source: Advanced Healthcare Materials ; Volume 10, Issue 3 , 2021 ; 21922640 (ISSN)
  9. URL: https://onlinelibrary.wiley.com/doi/abs/10.1002/adhm.202001122