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    Online analysis of local field potentials for seizure detection in freely moving rats

    , Article Iranian Journal of Basic Medical Sciences ; Volume 23, Issue 2 , 2020 , Pages 173-177 Zare, M ; Nazari, M ; Shojaei, A ; Raoufy, M. R ; Mirnajafi Zadeh, J ; Sharif University of Technology
    Mashhad University of Medical Sciences  2020
    Abstract
    Objective(s): Seizure detection during online recording of electrophysiological parameters is very important in epileptic patients. In the present study, online analysis of field potential recordings was used for detecting spontaneous seizures in epileptic animals. Materials and Methods: Epilepsy was induced in rats by pilocarpine injection. During the chronic period of the pilocarpine model, local field potential (LFP) recording was run for at least 24 hr. At the same time, video monitoring of the animals was done to determine the real time of seizure occurrence. Both power and sample entropy of LFP were used for online analysis. Results: Obtained results showed that changes in LFP power... 

    Early detection of immunization: A study based on an animal model using 1H nuclear magnetic resonance spectroscopy

    , Article Pakistan Journal of Biological Sciences ; Volume 14, Issue 3 , 2011 , Pages 195-203 ; 10288880 (ISSN) Zamani, Z ; Arjmand, M ; Tafazzoli, M ; Ghohzadeh, A ; Pourfallah, F ; Sadeghi, S ; Mirzazadeh, R ; Mirkham, F ; Tahen, S ; Iravam, A ; Bayat, P ; Vahabi, F ; Sharif University of Technology
    2011
    Abstract
    Vaccines require a period of at least three months for clinical trials, hence a method that can identify elicitation of immune response a few days after the first dose is a necessity. Evolutionary variable selections are modeling approaches for proper manipulation of available data which were used to set up an animal model for classification of time dependent 'HNMR metabolomic profiles and pattern recognition of fluctuations of metabolites in two groups of male rabbits. One group of rabbits was immunized with human red blood cells and the other used as control. Blood was obtained every 48 h from each rabbit for a period of six weeks and the serum monitored for antibodies and metabolites by... 

    Type V collagen in scar tissue regulates the size of scar after heart injury

    , Article Cell ; Volume 182, Issue 3 , 2020 , Pages 545-562.e23 Yokota, T ; McCourt, J ; Ma, F ; Ren, S ; Li, S ; Kim, T. H ; Kurmangaliyev, Y. Z ; Nasiri, R ; Ahadian, S ; Nguyen, T ; Tan, X. H. M ; Zhou, Y ; Wu, R ; Rodriguez, A ; Cohn, W ; Wang, Y ; Whitelegge, J ; Ryazantsev, S ; Khademhosseini, A ; Teitell, M. A ; Chiou, P. Y ; Birk, D. E ; Rowat, A. C ; Crosbie, R. H ; Pellegrini, M ; Seldin, M ; Lusis, A. J ; Deb, A ; Sharif University of Technology
    Cell Press  2020
    Abstract
    Scar tissue size following myocardial infarction is an independent predictor of cardiovascular outcomes, yet little is known about factors regulating scar size. We demonstrate that collagen V, a minor constituent of heart scars, regulates the size of heart scars after ischemic injury. Depletion of collagen V led to a paradoxical increase in post-infarction scar size with worsening of heart function. A systems genetics approach across 100 in-bred strains of mice demonstrated that collagen V is a critical driver of postinjury heart function. We show that collagen V deficiency alters the mechanical properties of scar tissue, and altered reciprocal feedback between matrix and cells induces... 

    Functional analyses of recombinant mouse hepcidin-1 in cell culture and animal model

    , Article Biotechnology Letters ; Volume 35, Issue 8 , August , 2013 , Pages 1191-1197 ; 01415492 (ISSN) Yazdani, Y ; Keyhanvar, N ; Kalhor, H. R ; Rezaei, A ; Sharif University of Technology
    2013
    Abstract
    Hepcidin is a peptide hormone that plays an important role in iron metabolism. We have produced a recombinant mouse hepcidin-1 by using baculovirus expression system. Its expression yield was 25 μg/ml when cell culture media were supplemented with a protease inhibitor cocktail. The recombinant mouse hepcidin-1 and synthetic human hepcidin-25 had similar effects on reducing ferroportin expression in J774A cell line and in peritoneal macrophages. However, synthetic human hepcidin-25 was more efficient than recombinant mouse hepcidin-1 in reducing iron concentration in blood circulation (p < 0.01)  

    The effect of temperature on eccentric contraction-induced isometric force loss in isolated perfused rat medial gastrocnemius muscle

    , Article Tehran University Medical Journal ; Volume 66, Issue 6 , 2008 , Pages 388-395 ; 16831764 (ISSN) Vasaghi Gharamaleki, B ; Keshavarz, M ; Gharibzadeh, S ; Marvi, H ; Mosayebnejad, J ; Ebrahimi Takamjani, E ; Sharif University of Technology
    Tehran University of Medical Sciences  2008
    Abstract
    Background: The typical features of eccentric exercise-induced muscle damage are delayed-onset muscle soreness (DOMS) and prolonged loss of muscle strength. It has been shown that passive warmth is effective in reducing muscle injury. Due to the interaction of different systems in vivo, we used isolated perfused medial gastrocnemius skeletal muscle to study the direct effect of temperature on the eccentric contraction-induced force loss. Methods: After femoral artery cannulation of a rat, the left medial gastrocnemius muscle was separated and then the entire lower limb was transferred into a prewarmed (35oC) chamber. With the chamber temperature at 31, 35 and 39oC before and during eccentric... 

    Antitumor effect of therapeutic HPV DNA vaccines with chitosan-based nanodelivery systems

    , Article Journal of Biomedical Science ; Vol. 21, issue. 1 , July , 2014 ; ISSN: 10217770 Tahamtan, A ; Ghaemi, A ; Gorji, A ; Kalhor, H. R ; Sajadian, A ; Tabarraei, A ; Moradi, A ; Atyabi, F ; Kelishadi, M ; Sharif University of Technology
    Abstract
    Cervical cancer is the second-most-common cause of malignancies in women worldwide, and the oncogenic activity of the human papilloma virus types (HPV) E7 protein has a crucial role in anogenital tumors. In this study, we have designed a therapeutic vaccine based on chitosan nanodelivery systems to deliver HPV-16 E7 DNA vaccine, considered as a tumor specific antigen for immunotherapy of HPV-associated cervical cancer. We have developed a Nano-chitosan (NCS) as a carrier system for intramuscular administration using a recombinant DNA vaccine expressing HPV-16 E7 (NCS-DNA E7 vaccine). NCS were characterized in vitro for their gene transfection ability. Results: The transfection of CS-pEGFP... 

    Accelerated full-thickness wound healing via sustained bFGF delivery based on a PVA/chitosan/gelatin hydrogel incorporating PCL microspheres

    , Article International Journal of Pharmaceutics ; Volume 537, Issue 1-2 , 2018 , Pages 278-289 ; 03785173 (ISSN) Shamloo, A ; Sarmadi, M ; Aghababaie, Z ; Vossoughi, M ; Sharif University of Technology
    Elsevier B.V  2018
    Abstract
    Herein, a hybrid hydrogel/microsphere system is introduced for accelerated wound healing by sustained release of basic fibroblast growth factor (bFGF). The hydrogel is composed of a mixture of PVA, gelatin and chitosan. The double-emulsion-solvent-evaporation method was utilized to obtain microspheres composed of PCL, as the organic phase, and PVA, as the aqueous phase. Subsequently, various in-vitro and in-vivo assays were performed to characterize the system. BSA was used to optimize the release mechanism, and encapsulation efficiency in microspheres, where a combination of 3% (w/v) PCL and 1% (w/v) PVA was found to be the optimum microsphere sample. Incorporation of microspheres within... 

    Fabrication and evaluation of chitosan/gelatin/PVA hydrogel incorporating honey for wound healing applications: An in vitro, in vivo study

    , Article International Journal of Pharmaceutics ; Volume 592 , 2021 ; 03785173 (ISSN) Shamloo, A ; Aghababaie, Z ; Afjoul, H ; Jami, M ; Bidgoli, M. R ; Vossoughi, M ; Ramazani, A ; Kamyabhesari, K ; Sharif University of Technology
    Elsevier B.V  2021
    Abstract
    In this study, physically cross-linked hydrogels were developed by freezing-thawing method while different concentrations of honey were included into the hydrogels for accelerated wound healing. The hydrogel was composed of chitosan, polyvinyl alcohol (PVA), and gelatin with the ratio of 2:1:1 (v/v), respectively. Further, the effect of honey concentrations on antibacterial properties, and cell behavior was investigated. In vivo studies, including wound healing mechanism using rat model and histological analysis of section tissue samples were performed. The results illustrated that the incorporation of honey in hydrogels increased the ultimate strain of hydrogels approximately two times,... 

    Allergic rhinitis impairs working memory in association with drop of hippocampal – Prefrontal coupling

    , Article Brain Research ; Volume 1758 , 2021 ; 00068993 (ISSN) Salimi, M ; Ghazvineh, S ; Nazari, M ; Dehdar, K ; Garousi, M ; Zare, M ; Tabasi, F ; Jamaati, H ; Salimi, A ; Barkley, V ; Mirnajafi Zadeh, J ; Raoufy, M. R ; Sharif University of Technology
    Elsevier B.V  2021
    Abstract
    Allergic rhinitis (AR) is a chronic inflammatory disease frequently associated with a deficit in learning and memory. Working memory is an important system for decision making and guidance, which depends on interactions between the ventral hippocampus (vHipp) and the prelimbic prefrontal cortex (plPFC). It is still unclear whether AR influences the activity and coupling of these brain areas, which consequently may impair working memory. The current study aimed to examine alterations of the vHipp-plPFC circuit in a rat model of AR. Our results show decreased working memory performance in AR animals, accompanied by a reduction of theta and gamma oscillations in plPFC. Also, AR reduces... 

    Global mortality of snakebite envenoming between 1990 and 2019

    , Article Nature Communications ; Volume 13, Issue 1 , 2022 ; 20411723 (ISSN) Roberts, N. L. S ; Johnson, E. K ; Zeng, S. M ; Hamilton, E. B ; Abdoli, A ; Alahdab, F ; Alipour, V ; Ancuceanu, R ; Andrei, C. L ; Anvari, D ; Arabloo, J ; Ausloos, M ; Awedew, A. F ; Badiye, A. D ; Bakkannavar, S. M ; Bhalla, A ; Bhardwaj, N ; Bhardwaj, P ; Bhaumik, S ; Bijani, A ; Boloor, A ; Cai, T ; Carvalho, F ; Chu, D.-T ; Couto, R. A. S ; Dai, X ; Desta, A. A ; Do, H. T ; Earl, L ; Eftekhari, A ; Esmaeilzadeh, F ; Farzadfar, F ; Fernandes, E ; Filip, I ; Foroutan, M ; Franklin, R. C ; Gaidhane, A. M ; Gebregiorgis, B. G ; Gebremichael, B ; Ghashghaee, A ; Golechha, M ; Hamidi, S ; Haque, S. E ; Hayat, K ; Herteliu, C ; Ilesanmi, O. S ; Islam, M. M ; Jagnoor, J ; Kanchan, T ; Kapoor, N ; Khan, E. A ; Khatib, M. N ; Khundkar, R ; Krishan, K ; Kumar, G. A ; Kumar, N ; Landires, I ; Lim, S. S ; Madadin, M ; Maled, V ; Manafi, N ; Marczak, L. B ; Menezes, R. G ; Meretoja, T. J ; Miller, T. R ; Mohammadian Hafshejani, A ; Mokdad, A. H ; Monteiro, F. N. P ; Moradi, M ; Nayak, V. C ; Nguyen, C. T ; Nguyen, H. L.T ; Nuñez-Samudio, V ; Ostroff, S. M ; Padubidri, J. R ; Pham, H. Q ; Pinheiro, M ; Pirestani, M ; Quazi Syed, Z ; Rabiee, N ; Radfar, A ; Rahimi Movaghar, V ; Rao, S. J ; Rastogi, P ; Rawaf, D. L ; Rawaf, S ; Reiner, R.C., Jr ; Sahebkar, A ; Samy, A. M ; Sawhney, M ; Schwebel, D. C ; Senthilkumaran, S ; Shaikh, M. A ; Skryabin, V. Y ; Skryabina, A. A ; Soheili, A ; Stokes, M. A ; Thapar, R ; Tovani Palone, M. R ; Tran, B. X ; Travillian, R. S ; Velazquez, D. Z ; Zhang, Z. J ; Naghavi, M ; Dandona, R ; Dandona, L ; James, S. L ; Pigott, D.M ; Murray, C. J. L ; Hay, S. I ; Vos, T ; Sharif University of Technology
    Nature Research  2022
    Abstract
    Snakebite envenoming is an important cause of preventable death. The World Health Organization (WHO) set a goal to halve snakebite mortality by 2030. We used verbal autopsy and vital registration data to model the proportion of venomous animal deaths due to snakes by location, age, year, and sex, and applied these proportions to venomous animal contact mortality estimates from the Global Burden of Disease 2019 study. In 2019, 63,400 people (95% uncertainty interval 38,900–78,600) died globally from snakebites, which was equal to an age-standardized mortality rate (ASMR) of 0.8 deaths (0.5–1.0) per 100,000 and represents a 36% (2–49) decrease in ASMR since 1990. India had the greatest number... 

    Oral administration of lithium chloride ameliorate spinal cord injury-induced hyperalgesia in male rats

    , Article PharmaNutrition ; Volume 21 , 2022 ; 22134344 (ISSN) Rahimi, G ; Mirsadeghi, S ; Rahmani, S ; Izadi, A ; Ghodsi, Z ; Ghodsi, S. M ; Rahimi Movaghar, V ; Kiani, S ; Sharif University of Technology
    Elsevier B.V  2022
    Abstract
    Background: Numerous studies have described the neuroprotective effect of lithium in spinal cord injury in addition to its ameliorative impact on pain sensation. In the present study, we aim to examine the efficacy of 85 mg/kg as well as 50 mg/kg dosage of the lithium chloride (LiCl) through oral consumption in spinal cord injured rats and their effect on gene expression of three candidate genes, corresponding to the hyper-sensitization. Methods: Adult Wistar (male) rats were divided into four experimental groups: control; oral administration of LiCl with 85 mg/kg and 50 mg/kg dosage; and 10 % sucrose receiver as the vehicle. BBB and heat plantar tests were performed weekly throughout four... 

    A unified approach for detection of induced epileptic seizures in rats using ECoG signals

    , Article Epilepsy and Behavior ; Volume 27, Issue 2 , 2013 , Pages 355-364 ; 15255050 (ISSN) Niknazar, M ; Mousavi, S. R ; Motaghi, S ; Dehghani, A ; Vosoughi Vahdat, B ; Shamsollahi, M. B ; Sayyah, M ; Noorbakhsh, S. M ; Sharif University of Technology
    2013
    Abstract
    Objective: Epileptic seizure detection is a key step for epilepsy assessment. In this work, using the pentylenetetrazole (PTZ) model, seizures were induced in rats, and ECoG signals in interictal, preictal, ictal, and postictal periods were recorded. The recorded ECoG signals were then analyzed to detect epileptic seizures in the epileptic rats. Methods: Two different approaches were considered in this work: thresholding and classification. In the thresholding approach, a feature is calculated in consecutive windows, and the resulted index is tracked over time and compared with a threshold. The moment the index crosses the threshold is considered as the moment of seizure onset. In the... 

    Application of a dissimilarity index of EEG and its sub-bands on prediction of induced epileptic seizures from rat's EEG signals

    , Article IRBM ; Volume 33, Issue 5-6 , December , 2012 , Pages 298-307 ; 19590318 (ISSN) Niknazar, M ; Mousavi, S. R ; Shamsollahi, M. B ; Vosoughi Vahdat, B ; Sayyah, M ; Motaghi, S ; Dehghani, A ; Noorbakhsh, S. M ; Sharif University of Technology
    2012
    Abstract
    Objective: Epileptic seizures are defined as manifest of excessive and hyper-synchronous activity of neurons in the cerebral cortex that cause frequent malfunction of the human central nervous system. Therefore, finding precursors and predictors of epileptic seizure is of utmost clinical relevance to reduce the epileptic seizure induced nervous system malfunction consequences. Researchers for this purpose may even guide us to a deep understanding of the seizure generating mechanisms. The goal of this paper is to predict epileptic seizures in epileptic rats. Methods: Seizures were induced in rats using pentylenetetrazole (PTZ) model. EEG signals in interictal, preictal, ictal and postictal... 

    کلیدواژه های تکراریCurcumin loading potentiates the neuroprotective efficacy of Fe3O4 magnetic nanoparticles in cerebellum cells of schizophrenic rats

    , Article Biomedicine and Pharmacotherapy ; Volume 108 , 2018 , Pages 1244-1252 ; 07533322 (ISSN) Naserzadeh, P ; Ashrafi Hafez, A ; Abdorahim, M ; Abdollahifar, M. A ; Shabani, R ; Peirovi, H ; Simchi, A ; Ashtari, K ; Sharif University of Technology
    Abstract
    Background: The aim of this study was to investigate the neurotoxic effects of Fe3O4 magnetic- CurNPs on isolated schizophrenia mitochondria of rats as an in vivo model. Methods: We designed CMN loaded superparamagnetic iron oxide nanoparticles (SPIONs) (Fe3O4 magnetic- CurNPs) to achieve an enhanced therapeutic effect. The physicochemical properties of Fe3O4 magnetic- CurNPs were characterized using X-ray diffraction (XRD), and dynamic laser light scattering (DLS) and zeta potential. Further, to prove Fe3O4 magnetic- CurNPs results in superior therapeutic effects, and also, the mitochondrial membrane potential collapse, mitochondrial complex II activity, reactive oxygen species generation,... 

    Simvastatin-loaded nano-niosomes confer cardioprotection against myocardial ischemia/reperfusion injury

    , Article Drug Delivery and Translational Research ; Volume 12, Issue 6 , 2022 , Pages 1423-1432 ; 2190393X (ISSN) Naseroleslami, M ; Mousavi Niri , N ; Akbarzade, I ; Sharifi, M ; Aboutaleb, N ; Sharif University of Technology
    Springer  2022
    Abstract
    Although simvastatin (SIM) has been proven to be a powerful agent against myocardial ischemia/reperfusion (MI/R) injury, poor water solubility, short half-life, and low bioavailability have made it futile while using conventional drug delivery system. Hence, this study aims to investigate therapeutic efficacy of SIM-loaded nano-niosomes on MI/R injury. Surface active agent film hydration method was used to synthesize nano-niosomes. The physicochemical properties of nano-niosomes were characterized using dynamic light scattering (DLS) and transmission electron microscopy (TEM). Moreover, niosomes were characterized in entrapment efficiency (EE) and releasing pattern. Male Wistar rats were... 

    Fullerene C60 nanoparticle attenuates pain and tumor necrosis factor-α protein expression in the hippocampus following diabetic neuropathy in rats

    , Article Physiology and Pharmacology (Iran) ; Volume 26, Issue 4 , 2022 , Pages 451-458 ; 24765236 (ISSN) Namdar, F ; Bahrami, F ; Bahari, Z ; Ghanbari, B ; Shahyad, S ; Mohammadi, M. T ; Sharif University of Technology
    Iranian Society of Physiology and Pharmacology  2022
    Abstract
    Introduction: Diabetic neuropathy is a common complication of diabetes mellitus. It is associated with nerve damage due to oxidative stress and high levels of pro-inflammatory mediators. In the present study, we examined the anti-nociceptive effects of Fullerene nanoparticle, as a potent anti-oxidant, during diabetic neuropathy. Methods: Diabetes mellitus induced through injection of streptozotocin (STZ) (40 mg/kg). Four groups were used in the study as follows: the control, control+fullerene, diabetes, and diabetes +fullerene groups. All four groups received sesame oil. Treatment rats received fullerene C60 (1mg/kg/day) for 9 weeks by intra-gastric gavage. Then, cold allodynia, histology,... 

    Evaluation of the effects of fullerene c60 nanoparticles on oxidative stress parameters in normal rats liver and brain

    , Article Journal of Advances in Medical and Biomedical Research ; Volume 27, Issue 124 , 2019 , Pages 8-15 ; 26766264 (ISSN) Namadr, F ; Bahrami, F ; Bahri, Z ; Ghanbari, B ; Elahi, S. A ; Mohammadi, M. T ; Sharif University of Technology
    Zanjan University of Medical Sciences and Health Services  2019
    Abstract
    Background & Objective: The potent antioxidant property of fullerene C60 nanoparticles and their derivatives has been demonstrated in a wide range of in vitro and in vivo studies. Hence, we examined the effects of fullerene C60 on the oxidative stress parameters in brain and liver of the rats in normal situation. Materials & Methods: The study was performed in two groups of Wistar rats (each group, n = 6); normal and fullerene-treated normal animals. Treated rats received fullerene via oral gavage at dose of 1 mg/kg/day for 60 days. At termination of the study, the oxidative stress parameters were determined in brain and liver tissues, including the contents of glutathione (GSH) and... 

    Disc size markedly influences concentration profiles of intravenously administered solutes in the intervertebral disc: A computational study on glucosamine as a model solute

    , Article European Spine Journal ; Vol. 23, issue. 4 , April , 2014 , p. 715-723 Motaghinasab, S ; Shirazi-Adl, A ; Parnianpour, M ; Urban, J. P. G ; Sharif University of Technology
    Abstract
    Purpose: Tests on animals of different species with large differences in intervertebral disc size are commonly used to investigate the therapeutic efficacy of intravenously injected solutes in the disc. We hypothesize that disc size markedly affects outcome. Methods: Here, using a small non-metabolized molecule, glucosamine (GL) as a model solute, we calculate the influence of disc size on transport of GL into rat, rabbit, dog and human discs for 10 h post intravenous-injection. We used transient finite element models and considered an identical GL supply for all animals. Results: Huge effects of disc size on GL concentration profiles were found. Post-injection GL concentration in the rat... 

    Alterations of the electroencephalogram sub-bands amplitude during focal seizures in the pilocarpine model of epilepsy

    , Article Physiology and Pharmacology ; Volume 16, Issue 1 , 2012 , Pages 11-20 ; 17350581 (ISSN) Motaghi, S ; Niknazar, M ; Sayyah, M ; babapour, V ; Vahdat, B. V ; Shamsollahi, M. B ; Sharif University of Technology
    2012
    Abstract
    Introduction: Temporal lobe epilepsy (TLE) is the most common and drug resistant epilepsy in adults. Due to behavioral, morphologic and electrographic similarities, pilocarpine model of epilepsy best resembles TLE. This study was aimed at determination of the changes in electroencephalogram (EEG) sub-bands amplitude during focal seizures in the pilocarpine model of epilepsy. Analysis of these changes might help detection of a pre-seizure state before an oncoming seizure. Methods: Rats were treated by scopolamine (1mg/kg, s.c) to prevent cholinergic effects. After 30 min, pilocarpine (380 mg/kg, i.p) was administered to induce status epilepticus (SE) and 2 hours after SE, diazepam (20 mg/kg,... 

    Combinational therapy of lithium and human neural stem cells in rat spinal cord contusion model

    , Article Journal of Cellular Physiology ; Volume 234, Issue 11 , 2019 , Pages 20742-20754 ; 00219541 (ISSN) Mohammadshirazi, A ; Sadrosadat, H ; Jaberi, R ; Zareikheirabadi, M ; Mirsadeghi, S ; Naghdabadi, Z ; Ghaneezabadi, M ; Fardmanesh, M ; Baharvand, H ; Kiani, S ; Sharif University of Technology
    Wiley-Liss Inc  2019
    Abstract
    A large number of treatment approaches have been used for spinal cord injury improvement, a medically incurable disorder, and subsequently stem cell transplantation appears to be a promising strategy. The main objective of this study is to ascertain whether combinational therapy of human neural stem cells (hNSCs) together with lithium chloride improves cell survival, proliferation, and differentiation in a rat spinal contusion model, or not. Contusive spinal cord injury was implemented on Wistar male rats. Experimental groups comprised of: control, hNSCs transplanted, lithium chloride (Li), and hNSCs and lithium chloride (hNSCs + Li). In every experimental group, locomotor activity score and...