Sharif Digital Repository

Lipases (Triacyl Glycerol Acyl Hydrolase, EC.3.1.1.3) are multidimensional biocatalysts which have many applications in food industrials, dairy, fats and oils processing, detergents, synthesis of pure chemical and pharmaceutical materials, paper industrial and cosmetics. Generally, industrial enzymes are produced extracellularly, so their separation from a low-cost complex medium (usually used for production) is one of the cost stages in their production process. Filtration is the most economical method of separation in the production of enzymes, but due to the small size of colloidal particles and cell masses present in the fermentation environment, its use is subject to limitations. In... 

Pretreatment of nuclear waste for valuable and limited uranium resources in the nuclear industry as well as environmental protection is imperative. Wash the affected parts of the Natanz uranium enrichment plant uranium centrifuges with a standard cleaning solution of citric acid, a liquid waste is generated in this study, the preparation of these residues using supercritical water oxidation (SCWO) and near critical (HTWO1) was studied as a result, the optimum operating conditions, and conditions of solvent extraction has been studied. In the result, the optimum operating conditions, and conditions of solvent extraction has been introduced. Also in the UCF2 as a liquid waste contaminated with... 

The purpose of this research is providing the preliminary study and designing, fabricating and installing diffuser vanes on the vaneless diffuser compressor to improve its overall performance. To meet the goal, the GT4082 turbocharger centrifugal compressor is chosen as the basis unit. The compressor is fully modeled, to predict the compressor performance curves. One dimensional and three dimensional models are provided, to simulate the compressor with vaneless diffuser as well as vaned diffuser. In one dimensional model, governing equations are continuity, energy, and state and also the empirical components losses coefficients of the compressor are used. In this method, fluid properties are... 

Compression systems are widely used in industry. Dynamic Modeling is a necessary step in design procedure of a safe compression system. A dynamic model should be adequate and simulate dominant elements of system. One important element of industrial compression systems is downstream pipelines. In this work, a dynamic model including pipeline acoustics is developed. It is accepted that changes of working gas molecular weight can cause inception of instabilities in compression systems. The dynamic model used to study mechanisms of these instabilities. A conventional control system is modeled and tuned for the compression system. The ability of this control system to prevent such instabilities... 

In this research, first, the performance map and surge line of 46M6 and 46MB8 multi-stage radial compressors, which have 6 and 8 stages, respectively, have been estimated and invarianted. Invarianting the performance map makes the performance curve and surge line of the compressor, unchanged from input conditions, including molecular weight, which is very important for control systems. Then, the hydrodynamic circuit of Compressor System C-3002 of Nouri Petrochemical Company was modeled according to the characteristics of its components by solving equations in MATLAB software. main working condition of the hydrodynamic circuit is close to Start of Life operating condition which has a... 

The present thesis investigates the surge phenomenon of high speed centrifugal compressors. Surge is a highly undesired condition and may cause severe damage to the machine and thus the plant. Traditionally, surge is avoided via a surge avoidance scheme wih recycle valve in discharge at the most common approach. A one dimensional technique is developed where the Greitzer lumped parameter model along with the Gravdahl one dimensional model is considered as the basis. Shaft dynamics, volume dynamics, losses, energy transfer and time lag are imbedded in the present model to improve the prediction of the compressor characteristics beyond the stability region. On the other hand, in order to... 

Gas turbines and centrifugal compressors are the preferred means of compressing the gas in pipeline systems. Both gas turbines and centrifugal compressors exhibit performance characteristics that depend on the operating point imposed on them by the pipeline operation. Simulation of gas turbine-driven centrifugal compressors is necessary for a variety of applications in the general area of pipeline simulation. This research focuses on modeling of twin shaft gas turbine dynamic and steady state performance. For this purpose, it was necessary to evaluate the thermodynamic working process of the gas turbine by using an in-house computer code. At design point, the calculations are solely based on... 

Pumps are widely used in oil and gas industry. Therefore condition monitoring for this type of equipment is very important. The motor current signature analysis (MCSA) technique as one of the condition monitoring techniques is very effective in the industry, but it is less used in industry. All conventional methods require additional sensors to be installed in the system; current signature analysis as a sensorless method can reduce the cost of condition monitoring. In this study, motor current signature analysis technique was used to diagnosis a centrifugal pump with a capacity of 187 kW from the refinery industry. The impeller wearing ring clearance problem has been investigated. This... 

The present work has been planned to elaborately investigate the key parameters which affect the formation of shrinkage porosities during centrifugal casting of ductile cast iron pipes. The porosity defects can be mostly found in the Hot Mould centrifugal casting products and adversely affect the final mechanical properties of ductile iron pipes namely Ultimate Tensile Strength and Elongation.
In the first point of view, the created defects were detected through microstructural observations and the corresponding responsible mechanisms were also discussed. The results showed that the inner porosity is produced because solidification occurs from both the inner and outer surfaces... 

Thermodynamic properties of supercritical carbon dioxide change near the critical point. High cycle efficiency is realized by having the main compressor to operate near the critical point of CO_2 to benefit from reduced compression work. In addition the high efficiency, compactness, nontoxicity, cheap working fluid and possibility of using dry cooling instead of wet cooling, s-CO_2 has many application such as nuclear, fossil fuel, renewable sources like solar thermal and waste heat. With regards to sCO_2 thermodynamic properties, suitable equation of state with high accuracy has been determined, and then is used in the design modeling. Recompression Brayton cycle with steady state flow is... 

The purpose of this study is developing the design means of a high pressure centrifugal compressor impeller for aviational applications i.e. turboshaft engines. The logic and constraints of designing a high pressure centrifugal impeller for a turboshaft engine is different from those of low pressure aerial or high speed industrial ones. This kind of impeller is deployed in a number of advanced generation of aero engines. In this study, the centrifugal impeller design tools are developed using two design methods. Then, the results are compared, validated and corrected with an existing high pressure impeller data. A number of impellers with pressure ratios ranging from 6 to 11 are designed and... 

Cell lysis, as the first procedure of cell pretreatment, is a process that breaks cell membranes open thereby facilitating access to intracellular substances, such as DNA, proteins, and other components for further analysis to diagnose and treat diseases at early stages. Currently, there are several methods for cell lysis at macroscales. However, the time-consuming and expensive procedures as well as the large scal of the system are the main disadvantages of the systems. Recently, microfluidic systems have attracted considerable attention due to advantages associated with automation, integration and miniaturization of biomedical test protocols. Centrifugal microfluidics (Lab-on-a-Disc) is a... 

The heart is a powerful muscle whose primary function is to pump blood into the body. many cardiac patients reach a stage that can no longer be kept function of their heart to a satisfactory level by using medication thus doctors undergo surgery. The common problem occurs to most patients undergoing cardiac surgery is that after the surgery, the patient's heart during a short period of time is unable to supply blood with the flow rate and pressure required for all organs, especially the end organs of the body. In this conditions, ventricular assist device help the patients. These devices are used parallel with patient’s heart and supply the large portion of blood that patient’s body needs.... 

Diabetes mellitus is a global endemic that is rapidly increasing prevalence in both developing and developed countries. Recently, the American Diabetes Association has recommended the hemoglobin A1c as a possible substitute to fasting blood glucose for diagnosis of diabetes because it is an indicator of long-term glycemic control. Also, centrifugal microfluidics systems have a good potential to be used in the point of care testing systems. In this study, two centrifugal microfluidic discs were designed and manufactured in order to separately measure the hemoglobin and hemoglobin A1c in the whole blood. In the first part of the study, the hemoglobin measurement disc consists of two chambers... 

Deoxyribonucleic acid (DNA) extraction, as one of the most important steps in modern molecular diagnostics, is the process by which DNA is separated from intracellular materials like proteins, membranes, and other materials contained in the cell. Microfluidic technology enables sophisticated, time-consuming and costly experiments with minimal use of raw materials, time and cost and acceptable accuracy. The predominant advantages of centrifugal microfluidic systems are utilizing centrifugal force to generate propulsion without the need for a pump, and eliminating the need for experts to run the system. Various fluidic operations such as valving, mixing, metering, heating, and sample... 

Radial Flow compressors have broad applications in aero-space, transport, gas and oil industries and etc. This type of compressor is a better choice in meeting constraints on weight and space, especially when continuous high pressure stream in low volumetric rate is of interest.
Through main components of radial flow compressors, many investigations have been performed on design and optimization of the impellers and the diffusers, but the optimum design and optimization of compressor volute have received less attention.Volute design has direct and strong influence on compressor performance and its stable operating range.
In this research numerical and experimental investigations is... 

In this research, an attempt is made to obtain the geometrical and performance matching between a centrifugal compressor and a pre-designed radial inflow turbine. In this regard, constraints such as the same outer diameters, stagnation pressure, rotational speed, and power are considered. The impeller of the centrifugal compressor is designed by employing one-dimensional design method. The aerodynamic performance of the centrifugal compressor is 3-dimensionally evaluated by the commercial CFX software. A comparison of the 1D and 3D results reveals a maximum deviation of 7% indicating relatively good agreement. A sensitivity analysis on the flow coefficient and tip clearance shows a change of... 

In this project a centrifugal, microfluidic platform to the end of separation and studying circulating tumor cells is designed, modelled and analyzed. Circulating tumor cells (CTCs) are specific types of cancer cells which leave the original tumor and enter the blood stream. For the separation of CTCs, a novel anti-body separation technic has been used which targets the specific MCF7 CTCs using their corresponding Ep-CAM antibody. First the Ep-CAM antibody is coated on magnetic nanoparticles through a chemical bonding process. Afterwards the CTCs are exposed to their antibodies, through which they will bind together and form CTCs with magnetic characteristics. Finally through exerting an... 

Overall performance improvement of gas turbine is available by modifying each component of cycle. The purpose of this research is to improve performance of a micro turbine with 200 KW power, by redesigning compressor set. Considerable increase in performance and stability occurs, by adding individual stage of axial compressor at the entry of centrifugal section. It should be noted that, this improvement occurs without a significant increase in size and without having to change the centrifugal compressor design.In this study, design of one stage axial compressor and three dimensional modeling is discussed after, modeling and preliminary design of centrifugal compressor. Then, after obtaining... 

Polymerase chain reaction method is a conventional method for obtaining multiple copies of a specified segment in the DNA molecule and to amplify the DNA molecule itself. Therefore, this method has been of paramount importance in different fields of research and has been applied for different applications. PCR requires thermal cycling, or repeated temperature changes between two or three discrete temperatures to amplify specific nucleic acid target sequences. To achieve such thermal cycling, conventional bench-top thermal cyclers generally use a metal heating block powered by Peltier elements or benefit from forced convection heat transfer. Although efforts have been made in order to reduce...