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Laccase activity assay using surface plasmon resonance band of gold nanoparticles formed by dopamine
, Article Plasmonics ; Volume 13, Issue 4 , 2018 , Pages 1409-1415 ; 15571955 (ISSN) ; Hormozi Nezhad, M. R ; Akhond, M ; Absalan, G ; Sharif University of Technology
Springer New York LLC
2018
Abstract
A simple, fast, and sensitive colorimetric technique for determination of laccase activity using dopamine (DA) induced growth of colloidal gold nanoparticles is proposed. It was found that the reduction of AuCl4 − to colloidal gold nanoparticles (AuNPs) by dopamine (DA) in the presence of citrate ion as stabilizing agent produced a very intense surface plasmon resonance peak of AuNPs at 530 nm. As the activity of laccase (at fixed concentration of DA) increases, the oxidation of DA to dopamine-o-quinone (DOQ) is enhanced. The latter product could not act as the reducing agent for the reduction of AuCl4 − to AuNPs. So, as the activity of laccase increases, the absorbance characteristic to the...
Laccase activity assay using surface plasmon resonance band of gold nanoparticles formed by dopamine
, Article Plasmonics ; 2017 , Pages 1-7 ; 15571955 (ISSN) ; Hormozi Nezhad, M. R ; Akhond, M ; Absalan, G ; Sharif University of Technology
Abstract
A simple, fast, and sensitive colorimetric technique for determination of laccase activity using dopamine (DA) induced growth of colloidal gold nanoparticles is proposed. It was found that the reduction of AuCl4 − to colloidal gold nanoparticles (AuNPs) by dopamine (DA) in the presence of citrate ion as stabilizing agent produced a very intense surface plasmon resonance peak of AuNPs at 530 nm. As the activity of laccase (at fixed concentration of DA) increases, the oxidation of DA to dopamine-o-quinone (DOQ) is enhanced. The latter product could not act as the reducing agent for the reduction of AuCl4 − to AuNPs. So, as the activity of laccase increases, the absorbance characteristic to the...