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histopathology
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Cancer Detection and Classification in Histopathology Images Under Small Training Set
, M.Sc. Thesis Sharif University of Technology ; Rohban, Mohammad Hossein (Supervisor) ; Sharifi Zarchi, Ali (Supervisor)
Abstract
Histopathology images are a type of medical images that are used to diagnose a variety of diseases. One of these illnesses is the Leukemia cancer, which has four different subtypes and is diagnosed using a blood smear image. As a result of the advancement of deep learning tools, models for diagnosing various types of disease from images have been developed in recent years.In this project, one of the best models developed to diagnose four different types of disease was replicated, and it was demonstrated that, while this model achieves acceptable accuracy, its decision is not based on medically significant criteria. In the following, a general method for diagnosing the disease is proposed...
Domain Generalization in Deep Learning Models for Histopathology
, M.Sc. Thesis Sharif University of Technology ; Rohban, Mohammad Hossein (Supervisor)
Abstract
Domain shift is an inevitable issue when histopathological images are analyzed in a standard laboratory. This is due to the variations in tissue handling, manual procedures for sample preparation, and differences in scanners. This can result in reduced performance of machine learning algorithms trained on images from one laboratory when applied to another. In this framework, the goal of utilizing domain generalization techniques in machine learning is to develop models that perform well in different domains. This research examines various methods of dealing with the domain shift challenges within the context of detecting mitotic cells, and proposes algorithms to improve domain generalization...
Association between human leukocyte antigens and cutaneous adverse drug reactions to antiepileptics and antibiotics in the Iranian population
, Article Dermatologic Therapy ; Volume 35, Issue 5 , 2022 ; 13960296 (ISSN) ; Rostami, A ; Firooz, A ; Esmaili, N ; Ghiasi, M ; Lajevardi, V ; Amirzargar, A. A ; Sheykhi, I ; Khamesipour, A ; Akhdar, M ; Sharif University of Technology
John Wiley and Sons Inc
2022
Abstract
In this case–control study, class І and ІІ human leukocyte antigen (HLA) alleles in Iranian patients with benign and severe cutaneous adverse drug reactions (CADRs) due to aromatic anticonvulsants and antibiotics were evaluated. Patients diagnosed with CADRs (based on clinical and laboratory findings) with a Naranjo score of ≥ 4 underwent blood sampling and HLA-DNA typing. The control group comprised 90 healthy Iranian adults. Alleles with a frequency of more than two were reported. Deviations from Hardy–Weinberg equilibrium were not observed. Eighty patients with CADRs including 54 females and 26 males with a mean age of 41.49 ± 16.08 years were enrolled in this study. The culprit drugs...
Opposite trends of GAS6 and GAS6-AS expressions in breast cancer tissues
, Article Experimental and Molecular Pathology ; Volume 118 , 2021 ; 00144800 (ISSN) ; Hussen, B. M ; Taheri, F ; Sattari, A ; Yousefi, H ; Omrani, M. D ; Nazer, N ; Ghafouri Fard, S ; Taheri, M ; Sharif University of Technology
Academic Press Inc
2021
Abstract
Growth arrest-specific gene 6 (GAS6) is a growth factor-like cytokine whose function is related with vitamin K. This protein interacts with receptor tyrosine kinase proteins such as Tyro3, Axl, and TAM Receptor family, therefore affecting the tumorigenic processes via different mechanisms. GAS6-antisense 1 (GAS6-AS1) is a long non-coding RNAs (lncRNAs) that is transcribed from a genomic regions nearby GAS6. This lncRNA is also implicated in the pathobiology of cancer. We intended to judge the role of GAS6 and GAS6-AS1 in the pathogenesis of breast cancer through appraisal of their expression levels in breast cancer tissues and their paired neighboring non-cancerous samples. Expression of...
Oncolytic newcastle disease virus delivered by mesenchymal stem cells-engineered system enhances the therapeutic effects altering tumor microenvironment
, Article Virology Journal ; Volume 17, Issue 1 , 2020 ; Ebrahimzadeh, M. S ; Miri, S. M ; Dianat Moghadam, H ; Ghorbanhosseini, S. S ; Mohebbi, S. R ; Keyvani, H ; Ghaemi, A ; Sharif University of Technology
BioMed Central Ltd
2020
Abstract
Background: Human papillomavirus (HPV)-associated malignancy remain a main cause of cancer in men and women. Cancer immunotherapy has represented great potential as a new promising cancer therapeutic approach. Here, we report Mesenchymal stem cells (MSCs) as a carrier for the delivery of oncolytic Newcastle disease virus (NDV) for the treatment of HPV-associated tumor. Methods: For this purpose, MSCs obtained from the bone marrow of C57BL mice, then cultured and characterized subsequently by the flow cytometry analysis for the presence of cell surface markers. In this study, we sought out to determine the impacts of MSCs loaded with oncolytic NDV on splenic T cell and cytokine immune...
Fabrication and evaluation of a bilayer hydrogel-electrospinning scaffold prepared by the freeze-gelation method
, Article Journal of Biomechanics ; Volume 98 , 2020 ; Shamloo, A ; Sharif University of Technology
Elsevier Ltd
2020
Abstract
This study presents a bilayer structure as a skin scaffold comprised of an electrospun sheet layer made of polycaprolactone and polyvinil alcohol and a porous hydrogel layer made of chitosan and gelatin. The hydrogel layer was fabricated by employing the freeze-gelation technique. The bilayer structure was achieved by pouring the hydrogel solution on the electrospun sheet at the bottom of a mold followed by the freeze-gelation technique to obtain a porous structure in the hydrogel. The hydrogel and hydrogel-electrospun samples were characterized by scanning electron microscopy, swelling, tensile strength, in vitro and in vivo analyses. From a mechanical strength standpoint, the combination...
Sustainable release of vancomycin from silk fibroin nanoparticles for treating severe bone infection in rat tibia osteomyelitis model
, Article ACS Applied Materials and Interfaces ; Volume 9, Issue 6 , 2017 , Pages 5128-5138 ; 19448244 (ISSN) ; Mottaghitalab, F ; Eslami, M ; Gholami, M ; Kundu, S. C ; Kaplan, D. L ; Farokhi, M ; Sharif University of Technology
American Chemical Society
2017
Abstract
The successful treatment of bone infections is a major challenge in the field of orthopedics. There are some common methods for treating bone infections, including systemic antibiotic administration, local nondegradable drug vehicles, and surgical debridement, and each of these approaches has advantages and disadvantages. In the present study, the antibiotic vancomycin (VANCO) was loaded in silk fibroin nanoparticles (SFNPs) and the complexes were then entrapped in silk scaffolds to form sustained drug delivery systems. The release kinetics of VANCO from SFNPs alone and when the SFNPs were entrapped in silk scaffolds were assessed at two different pH values, 4.5 and 7.4, that affected the...
Delivery of melittin-loaded niosomes for breast cancer treatment: an in vitro and in vivo evaluation of anti-cancer effect
, Article Cancer Nanotechnology ; Volume 12, Issue 1 , 2021 ; 18686958 (ISSN) ; Akbarzadeh, I ; Marzbankia, E ; Farid, M ; khaledi, L ; Reihani, A. H ; Javidfar, M ; Mortazavi, P ; Sharif University of Technology
BioMed Central Ltd
2021
Abstract
Background: Melittin, a peptide component of honey bee venom, is an appealing candidate for cancer therapy. In the current study, melittin, melittin-loaded niosome, and empty niosome had been optimized and the anticancer effect assessed in vitro on 4T1 and SKBR3 breast cell lines and in vivo on BALB/C inbred mice. "Thin-layer hydration method" was used for preparing the niosomes; different niosomal formulations of melittin were prepared and characterized in terms of morphology, size, polydispersity index, encapsulation efficiency, release kinetics, and stability. A niosome was formulated and loaded with melittin as a promising drug carrier system for chemotherapy of the breast cancer cells....
Overexpression of protein kinase Mζ in the hippocampus mitigates alzheimer's disease-related cognitive deficit in rats
, Article Brain Research Bulletin ; Volume 166 , 2021 , Pages 64-72 ; 03619230 (ISSN) ; Roosta Azad, R ; Motamedi, F ; Mirzapour Delavar, H ; Ghasemi, S ; Aliakbari, S ; Pourbadie, H. G ; Sharif University of Technology
Elsevier Inc
2021
Abstract
Accumulation of amyloid beta (Aβ) soluble forms in the cerebral parenchyma is the mainstream concept underlying memory deficit in the early phase of Alzheimer's disease (AD). PKMζ plays a critical role in the maintenance of long-term memory. Yet, the role of this brain-specific enzyme has not been addressed in AD. We examined the impact of hippocampal PKMζ overexpression on AD-related memory impairment in rats. Oligomeric form of Aβ (oAβ) or vehicle was bilaterally microinjected into the dorsal hippocampus of male Wistar rats under stereotaxic surgery. One week later, 2 μl of lentiviral vector (108 T.U. / ml.) encoding PKMζ genome was microinjected into the dorsal hippocampus. Seven days...
Glypican-1 overexpression in different types of breast cancers
, Article OncoTargets and Therapy ; Volume 14 , 2021 , Pages 4309-4318 ; 11786930 (ISSN) ; Al Saraireh, Y. M ; Youssef, A. M. M ; Al Sarayra, Y. M ; Alrawashdeh, H. M ; Sharif University of Technology
Dove Medical Press Ltd
2021
Abstract
Purpose: Treatment of metastatic breast cancer patients is challenging and remains a major underlying cause of female mortality. Understanding molecular alterations in tumor development is critical to identify novel biomarkers and targets for cancer diagnosis and therapy. One of the aberrant cancer expressions gaining recent research interest is glypican-1. Several studies reported strong glypican-1 expression in various types of human cancers. However, none of these investigated glypican-1 expression in a large cohort of breast cancer histopathological subtypes. Patients and Methods: Immunohistochemistry was used to assess glypican-1 expression in 220 breast cancer patients and its relation...
Cytochrome 4z1 expression is correlated with poor prognosis in patients with cervical cancer
, Article Current Oncology ; Volume 28, Issue 5 , 2021 , Pages 3573-3584 ; 17187729 (ISSN) ; Alshammari, F. O. F. O ; Youssef, A. M. M ; Al Sarayra, Y. M ; Al Saraireh, R. A ; Al Muhaisen, G. H ; Al Mahdy, Y. S ; Al Kharabsheh, A. M ; Abufraijeh, S. M ; Al Rawashdeh, H. M ; Sharif University of Technology
MDPI
2021
Abstract
Background: cervical cancer is one of the most common malignancies in women worldwide and its management remains challenging and complex. As Cytochrome4Z1 (CYP4Z1) is overex-pressed in many tumours, its expression in cervical cancer is unknown. Therefore, the present study aimed to evaluate CYP4Z1 expression in cervical cancers. Methods: CYP4Z1 expression was immunohistochemically assessed in 100 cases of cervical cancers along with ten normal cervix tissues, and the enzyme’s relationship to several clinicopathological features and survival was explored. Results: CYP4Z1 was strongly expressed in 55% of cervical cancer patients. Normal cervix samples were negative for CYP4Z1 expression....
Cytochrome 4Z1 expression is associated with unfavorable survival in triple-negative breast cancers
, Article Breast Cancer: Targets and Therapy ; Volume 13 , 2021 , Pages 565-574 ; 11791314 (ISSN) ; Alshammari, F. O. F. O ; Youssef, A. M. M ; Al Tarawneh, F ; Al Sarayreh, S ; Almuhaisen, G. H ; Satari, A. O ; Al Shuneigat, J ; Alrawashdeh, H. M ; Sharif University of Technology
Dove Medical Press Ltd
2021
Abstract
Purpose: Triple-negative breast cancer (TNBC) is characterized by high mortality rate, and its clinical management is difficult and complex. Therefore, there is a need for extensive efforts aimed at accelerating the discovery of novel therapies for TNBC. CYP4Z1 has been implicated in the development of breast cancer. The current study aimed at characterizing the expression of CYP4Z1 on TNBC. Materials and Methods: Using immunohistochemistry, CYP4Z1 expression was evaluated on 122 TNBC samples, four samples of breast cancers expressing ER, PR, and HER-2, and four samples of normal breast tissues. The association between the enzyme and various histopathological features and survival of...
Screening of glypican-6 expression in benign, primary and metastatic colon cancers
, Article Clinical Medicine Insights: Oncology ; Volume 15 , 2021 ; 11795549 (ISSN) ; Alshammari, F. O. F. O ; Youssef, A. M. M ; Al Sarayreh, S ; Al Sarayra, Y. M ; Aborajooh, E ; Al Shuneigat, J ; Alrawashdeh, H. M ; Sharif University of Technology
SAGE Publications Ltd
2021
Abstract
Background: The development of colon cancer has been described as a multistep process of carcinogenesis. Understanding molecular and cellular changes underlying this process is required to determine potential biomarkers and therapeutic targets in colon cancers. Several molecular entities, including glypicans, are implicated in cancer development. Among these is glypican-6, which is overexpressed in a limited number of cancers. This study aims to characterise the glypican-6 expression in different types of colon cancer. Methods: Immunohistochemistry was used to characterise glypican-6 expression in a panel of archived formalin-fixed, paraffin-embedded colon tissue types. These types included...