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kartogenin
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Design and Fabrication of Three Dimensional Scaffold Using Bio-Printing Technique for Cartilage Tissue Engineering
, M.Sc. Thesis Sharif University of Technology ; Mashayekhan, Shohreh (Supervisor) ; Hassanzadeh, Zabiollah (Co-Supervisor)
Abstract
Herein, we successfully prepared two types of hydrogels, alginate-beta-cyclodextrin based hydrogel and alginate without beta-cyclodextrin hydrogel in order to use in cartilage tissue engineering. At first, sodium alginate was modified with beta-cyclodextrin ring due to incorporate the kartogenin (KGN) differentiating drug in the hydrogel structure. For confirming the correctness of the modification reactions, H-NMR spectroscopy was used. In the presence of phenolized-ECM, alginic acid, modified alginate with beta cyclodextrin, HRP, H2O2, calcium carbonate, and glucono-delta-lactone (GDL), ECM-alginate-IPN and ECM-modified-alginate-IPN hydrogels were synthesized. Then, the KGN was loaded on...
Injectable in situ forming kartogenin-loaded chitosan hydrogel with tunable rheological properties for cartilage tissue engineering
, Article Colloids and Surfaces B: Biointerfaces ; Volume 192 , 2020 ; Chen, Y ; Wang, D ; Bagheri, R ; Solouk, A ; Wu, H ; Sharif University of Technology
Elsevier B.V
2020
Abstract
Limited regeneration capacity of cartilage can be addressed by tissue engineering approaches including localized delivery of bioactive agents using biomaterials. Although chitosan hydrogels have been considered as appropriate candidates for these purposes, however, their poor mechanical properties limit their real applications. Here, we develop in situ forming chitosan hydrogels with enhanced shear modulus by chemical modification of chitosan using N-(β-maleimidopropyloxy) succinimide ester (BMPS). Moreover, we utilize β-Glycerophosphate (β-GP) in the hydrogels for achieving thermosensitivity. We investigate the effects of BMPS, β-GP and chitosan concentration on rheological and swelling...
A dual functional chondro-inductive chitosan thermogel with high shear modulus and sustained drug release for cartilage tissue engineering
, Article International Journal of Biological Macromolecules ; Volume 205 , 2022 , Pages 638-650 ; 01418130 (ISSN) ; Mehrjou, B ; Wang, D ; Bagheri, R ; Solouk, A ; Chu, P. K ; Wu, H ; Sharif University of Technology
Elsevier B.V
2022
Abstract
We report a chitosan-based nanocomposite thermogel with superior shear modulus resembling that of cartilage and dual pro-chondrogenic and anti-inflammatory functions. Two therapeutic agents, kartogenin (KGN) and diclofenac sodium (DS), are employed to promote chondrogenesis of stem cells and suppress inflammation, respectively. To extend the release time in a controlled manner, KGN is encapsulated in the uniform-sized starch microspheres and DS is loaded into the halloysite nanotubes. Both drug carriers are doped into the maleimide-modified chitosan hydrogel to produce a shear modulus of 167 ± 5 kPa that is comparable to that of articular cartilage (50–250 kPa). Owing to the hydrogel...