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Dielectrophoretic cell sorting via sliding cells on 3D silicon microelectrodes
, Article Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 22 January 2017 through 26 January 2017 ; 2017 , Pages 147-150 ; 10846999 (ISSN) ; 9781509050789 (ISBN) ; Chan, M. L ; Roshan, K. A ; Yobas, L ; Sharif University of Technology
Abstract
This work presents an innovative design for a flow-through dielectrophoretic cell sorting based on silicon bulk microelectrodes featuring sidewall undercuts. The microelectrodes are configured into an interdigitated array with digits extending across the flow chamber at an oblique angle against the flow stream. Target cells under dielectrophoretic forces and hydrodynamic drag can slide along the digits to a dedicated outlet. The design has been showcased for continuous-flow sorting of viable and non-viable mammalian cells, achieving a throughput of 16,600 cells/min, an order of magnitude higher than those reported for existing continuous-flow cell sorting designs using thin-film or...
Improved green biosynthesis of chitosan decorated Ag- and Co3O4-nanoparticles: A relationship between surface morphology, photocatalytic and biomedical applications
, Article Nanomedicine: Nanotechnology, Biology, and Medicine ; Volume 32 , 2021 ; 15499634 (ISSN) ; Rabiee, N ; Bagherzadeh, M ; Ghadiri, A.M ; Fatahi, Y ; Dinarvand, R ; Webster, T. J ; Sharif University of Technology
Elsevier Inc
2021
Abstract
AgNPs@Chitosan and Co3O4-NPs@Chitosan were fabricated with Salvia hispanica. Results showed MZI values of 5 and 30 mm for Co3O4-NPs- and AgNPs@Chitosan against S. aureus, and 15 and 21 mm for Co3O4-NPs- and AgNPs@Chitosan against E. coli (24 h, 20 μg/mL), respectively. MTT assays showed up to 80% and 90%, 71% and 75%, and 91% and 94% mammalian cell viability for the green synthesized, chemically synthesized AgNPs and green synthesized AgNPs@Chitosan for HEK-293 and PC12 cells, respectively, and 70% and 71%, 59% and 62%, and 88% and 73% for the related Co3O4-NPs (24 h, 20 μg/mL). The photocatalytic activities showed dye degradation after 135 and 105 min for AgNPs@Chitosan and...