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microfluidics
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Comparison of logarithmic, elliptic, and conical helical spiral for isolation of circulating tumor cells based on inertial method
, Article Physics of Fluids ; Volume 34, Issue 9 , 2022 ; 10706631 (ISSN) ; Mozhdehbakhsh Mofrad, Y ; Safari, M ; Naseri, T ; Sharif University of Technology
American Institute of Physics Inc
2022
Abstract
Cancer is one of the most significant causes of death in the world. It has been shown that the role of circulating tumor cells (CTCs) in the early detection of cancer is crucial. Since the number of these cancerous cells in blood is very rare, the inertial microfluidic devices are one of the best candidates for the isolation of CTCs because they result in a high throughput process. Consequently, they can process a large volume of blood in a short time. Despite extensive computational and experimental studies on inertial microfluidic platforms, the impact of the curvature has not been thoroughly investigated during separation. In this paper, the feasibility of isolation of CTCs for...
Chlorophyll-based wicking sensing bioplatform coupled with a smartphone-based sample-to-answer analytical device for on-site detection of picric acid
, Article Biosensors and Bioelectronics: X ; Volume 11 , 2022 ; 25901370 (ISSN) ; Abbasi Moayed, S ; Hamzei, Z ; Keshavarz, A ; Yousefi, S ; Hormozi Nezhad, M. R ; Golmohammadi, H ; Sharif University of Technology
Elsevier Ltd
2022
Abstract
The development of inexpensive, equipment-free, portable, and user/environmentally-friendly analytical devices that can be efficiently utilized for on-site detection of PA is still a great challenge. To address these unmet demands, we have developed a smartphone-based disposable sensing bioplatform through immobilization of chlorophyll in a cotton thread for fluorescent monitoring of picric acid (PA). The fluorescence quenching of chlorophyll upon increasing the PA concentration based on the inner filter effect was considered as the sensing strategy in the developed sensor. The fabricated thread-based microfluidic sensing bioplatform was further coupled with a compact, low cost, portable,...
Particles focusing and separation by a novel inertial microfluidic device: divergent serpentine microchannel
, Article Industrial and Engineering Chemistry Research ; Volume 61, Issue 38 , 2022 , Pages 14324-14333 ; 08885885 (ISSN) ; Shamloo, A ; Vatani, P ; Ebrahimi, S ; Sharif University of Technology
American Chemical Society
2022
Abstract
Microfluidic experiments have found wide applications in medical sciences and engineering, such as cell separation and focusing. In the present study, focusing and separation of particles with different sizes and densities were investigated by designing inertial microfluidic devices. The microfluidic channel is designed by analyzing the induced forces on the particles. In the designing process, the objective was to focus and separate the particles in the shortest length of the channel with the lowest possible cycles and high efficiency. The simulation is then used for analyzing the two proposed geometries to evaluate their particle separation and focusing ability, named convergent and...
The effect of non-uniform magnetic field on the efficiency of mixing in droplet-based microfluidics: a numerical investigation
, Article Micromachines ; Volume 13, Issue 10 , 2022 ; 2072666X (ISSN) ; Nouri, M ; Hassani Gangaraj, M ; Shamloo, A ; Nasiri, R ; Sharif University of Technology
MDPI
2022
Abstract
Achieving high efficiency and throughput in droplet-based mixing over a small characteristic length, such as microfluidic channels, is one of the crucial parameters in Lab-on-a-Chip (LOC) applications. One solution to achieve efficient mixing is to use active mixers in which an external power source is utilized to mix two fluids. One of these active methods is magnetic micromixers using ferrofluid. In this technique, magnetic nanoparticles are used to make one phase responsive to magnetic force, and then by applying a magnetic field, two fluid phases, one of which is magneto-responsive, will sufficiently mix. In this study, we investigated the effect of the magnetic field’s characteristics...
Recent approaches to mRNA vaccine delivery by lipid-based vectors prepared by continuous-flow microfluidic devices
, Article Future medicinal chemistry ; Volume 14, Issue 21 , 2022 , Pages 1561-1581 ; 17568927 (ISSN) ; Tavakoli, A ; Mohammadghasemi, H ; Karimi, A ; Ai, J ; Rabiee, M ; Rabiee, N ; Sharif University of Technology
NLM (Medline)
2022
Abstract
Advancements in nanotechnology have resulted in the introduction of several nonviral delivery vectors for the nontoxic, efficient delivery of encapsulated mRNA-based vaccines. Lipid- and polymer-based nanoparticles (NP) have proven to be the most potent delivery systems, providing increased delivery efficiency and protection of mRNA molecules from degradation. Here, the authors provide an overview of the recent studies carried out using lipid NPs and their functionalized forms, polymeric and lipid-polymer hybrid nanocarriers utilized mainly for the encapsulation of mRNAs for gene and immune therapeutic applications. A microfluidic system as a prevalent methodology for the preparation of NPs...
Microfluidic-assisted fiber production: Potentials, limitations, and prospects
, Article Biomicrofluidics ; Volume 16, Issue 6 , 2022 ; 19321058 (ISSN) ; Nilghaz, A ; Saadatmand, M ; Naeimirad, M ; Demello, A. J ; Sharif University of Technology
American Institute of Physics Inc
2022
Abstract
Besides the conventional fiber production methods, microfluidics has emerged as a promising approach for the engineered spinning of fibrous materials and offers excellent potential for fiber manufacturing in a controlled and straightforward manner. This method facilitates low-speed prototype synthesis of fibers for diverse applications while providing superior control over reaction conditions, efficient use of precursor solutions, reagent mixing, and process parameters. This article reviews recent advances in microfluidic technology for the fabrication of fibrous materials with different morphologies and a variety of properties aimed at various applications. First, the basic principles, as...
A new 3D, microfluidic-oriented, multi-functional, and highly stretchable soft wearable sensor
, Article Scientific Reports ; Volume 12, Issue 1 , 2022 ; 20452322 (ISSN) ; Esmaeili Dokht, P ; Olyanasab, A ; Orouji, N ; Alipour, Z ; Sayad, M. H ; Rajabi, K ; Mazzolai, B ; Fardmanesh, M ; Sharif University of Technology
Nature Research
2022
Abstract
Increasing demand for wearable devices has resulted in the development of soft sensors; however, an excellent soft sensor for measuring stretch, twist, and pressure simultaneously has not been proposed yet. This paper presents a novel, fully 3D, microfluidic-oriented, gel-based, and highly stretchable resistive soft sensor. The proposed sensor is multi-functional and could be used to measure stretch, twist, and pressure, which is the potential of using a fully 3D structure in the sensor. Unlike previous methods, in which almost all of them used EGaIn as the conductive material, in this case, we used a low-cost, safe (biocompatible), and ubiquitous conductive gel instead. To show the...
Label-free electrochemical microfluidic biosensors: futuristic point-of-care analytical devices for monitoring diseases
, Article Microchimica Acta ; Volume 189, Issue 7 , 2022 ; 00263672 (ISSN) ; Samadi Pakchin, P ; Shamloo, A ; Mota, A ; de la Guardia, M ; Omidian, H ; Omidi, Y ; Sharif University of Technology
Springer
2022
Abstract
The integration of microfluidics with electrochemical analysis has resulted in the development of single miniaturized detection systems, which allows the precise control of sample volume with multianalyte detection capability in a cost- and time-effective manner. Microfluidic electrochemical sensing devices (MESDs) can potentially serve as precise sensing and monitoring systems for the detection of molecular markers in various detrimental diseases. MESDs offer several advantages, including (i) automated sample preparation and detection, (ii) low sample and reagent requirement, (iii) detection of multianalyte in a single run, (iv) multiplex analysis in a single integrated device, and (v)...
Analytical and numerical study on droplet breakup in microfluidic T-junction
, Article Chemical Engineering and Processing - Process Intensification ; Volume 177 , 2022 ; 02552701 (ISSN) ; Moosavi, A ; Hannani, S. K ; Sharif University of Technology
Elsevier B.V
2022
Abstract
Droplet breakup in symmetric T-junctions is one of the important phenomena in droplet microfluidics. Many studies have been done about the droplet breakup but none of them has provided a general analytical solution for the droplet breakup. In this study, we present an analytical solution to investigate the effect of important parameters such as the capillary number, droplet length, and channel widths ratio. The analytical solution is validated using the numerical simulation. Using the analytical equation and numerical results, we propose a generalized relationship. This general equation can be used as a rule of thumb for droplet breakup predictions with high accuracy. The results indicate...
Developing an Off-the-Shelf microfluidic droplet generation device for cell encapsulation
, Article Industrial and Engineering Chemistry Research ; Volume 61, Issue 30 , 2022 , Pages 10689-10699 ; 08885885 (ISSN) ; Shamloo, A ; Sharif University of Technology
American Chemical Society
2022
Abstract
Droplet microfluidics is a platform of microfluidics in which two immiscible fluids are used to generate droplets for various biomedical applications. This platform introduces several advantages in applications such as cell lysis, cell culture, co-culture, and cell encapsulation. The most important issues regarding droplet generation devices are the fabrication complexity and maintenance of these devices. In this study, a simple and easy-to-fabricate microdroplet generator is designed and fabricated to resolve these issues. Furthermore, since this device is easy to fabricate and use, it can play a key role in the fabrication of medical devices for controlling infectious diseases in poor and...
Cost-effective 3D H-filter fabricated by xurographic method
, Article Microfluidics and Nanofluidics ; Volume 26, Issue 9 , 2022 ; 16134982 (ISSN) ; Mirzaei, A ; Taghipoor, M ; Sharif University of Technology
Springer Science and Business Media Deutschland GmbH
2022
Abstract
In this paper, the conventional 2D H-filter is modified to 3D multi-layer structures fabricated by low-cost xurography. The extraction efficiency was studied experimentally for different 3D structures and then compared with conventional 2D H-filter. Numerical simulations were performed to model the diffusion flow in the proposed H-filters, showing a good agreement with the experimental observations. Results show that 3D H-filters have higher extraction efficiency at the same Reynolds number. Moreover, these structures could benefit low-cost and commercial applications due to their higher efficiency at smaller sizes. © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH...
An integrative method to increase the reliability of conventional double emulsion method
, Article Analytica Chimica Acta ; Volume 1197 , 2022 ; 00032670 (ISSN) ; Afjoul, H ; Shamloo, A ; Sharif University of Technology
Elsevier B.V
2022
Abstract
Polymeric microspheres which can load biomolecules, cells and active agents play an important role in tissue engineering and drug delivery systems. The conventional double emulsion method has been frequently used to fabricate polymeric microspheres. However, this method has two major shortcomings: the complicated fabrication process which makes it difficult to predict the characteristics of the final microspheres while the size distribution of the microspheres has a wide range. In this study, we eliminate the shortcomings of the conventional double emulsion method and increase its performance without decreasing its high production rate. This can make the proposed modified method a promising...
Unraveling cancer metastatic cascade using microfluidics-based technologies
, Article Biophysical Reviews ; Volume 14, Issue 2 , 2022 , Pages 517-543 ; 18672450 (ISSN) ; Kermanshah, L ; Abouali, H ; Hashemi, H. M ; Yari, A ; Khorasheh, F ; Alemzadeh, I ; Vossoughi, M ; Sharif University of Technology
Springer Science and Business Media Deutschland GmbH
2022
Abstract
Cancer has long been a leading cause of death. The primary tumor, however, is not the main cause of death in more than 90% of cases. It is the complex process of metastasis that makes cancer deadly. The invasion metastasis cascade is the multi-step biological process of cancer cell dissemination to distant organ sites and adaptation to the new microenvironment site. Unraveling the metastasis process can provide great insight into cancer death prevention or even treatment. Microfluidics is a promising platform, that provides a wide range of applications in metastasis-related investigations. Cell culture microfluidic technologies for in vitro modeling of cancer tissues with fluid flow and the...
Computational study of an integrated microfluidic device for active separation of RBCs and cell lysis
, Article Chemical Engineering and Processing - Process Intensification ; Volume 174 , 2022 ; 02552701 (ISSN) ; Shamloo, A ; Gangaraj, M. H ; Sharif University of Technology
Elsevier B.V
2022
Abstract
Separation and lysis of RBCs play an important role in diagnosis of different diseases. Although they have been partially studied in several researches, a comprehensive study on integrating both separation and lysis units on a single chip has been seen rarely in the literature. Also, the factors related to the chemical lysis process have not been investigated in detail. In this study, we introduce a novel microfluidic channel design for sequential RBC's separation and lysis. For the separation part, an active method with an electric field was applied to the cells. Besides, a novel mixer was designed for mixing the cell solution and lysis reagent. In the lysis section, we used a mathematical...
Droplet-based microfluidics in biomedical applications
, Article Biofabrication ; Volume 14, Issue 2 , 2022 ; 17585082 (ISSN) ; Besanjideh, M ; Nasiri, R ; Shamloo, A ; Nasrollahi, F ; De Barros, N. R ; Davoodi, E ; Erdem, A ; Mahmoodi, M ; Hosseini, V ; Montazerian, H ; Jahangiry, J ; Darabi, M.A ; Haghniaz, R ; Dokmeci, M.R ; Annabi, N ; Ahadian, S ; Khademhosseini, A ; Sharif University of Technology
IOP Publishing Ltd
2022
Abstract
Droplet-based microfluidic systems have been employed to manipulate discrete fluid volumes with immiscible phases. Creating the fluid droplets at microscale has led to a paradigm shift in mixing, sorting, encapsulation, sensing, and designing high throughput devices for biomedical applications. Droplet microfluidics has opened many opportunities in microparticle synthesis, molecular detection, diagnostics, drug delivery, and cell biology. In the present review, we first introduce standard methods for droplet generation (i.e. passive and active methods) and discuss the latest examples of emulsification and particle synthesis approaches enabled by microfluidic platforms. Then, the applications...
Microfluidics experimental investigation of the mechanisms of enhanced oil recovery by low salinity water flooding in fractured porous media
, Article Fuel ; Volume 314 , 2022 ; 00162361 (ISSN) ; Fatemi, M ; Masihi, M ; Sharif University of Technology
Elsevier Ltd
2022
Abstract
Spontaneous imbibition of water from fracture into the matrix is considered as one of the most important recovery mechanisms in the fractured porous media. However, water cannot spontaneously imbibe into the oil-wet rocks and as a result oil won't be produced, unless the capillary pressure barrier between fracture conduits and matrix is overcome. Wettability alteration is known as the main affecting mechanism for low salinity water flooding (LSWF), however, its effectiveness in fractured porous media has been less investigated, especially in the case of possible pore scale displacement mechanisms. In this study, the effectiveness of LSWF (diluted seawater) on oil recovery is compared to the...
Design of two Inertial-based microfluidic devices for cancer cell separation from Blood: A serpentine inertial device and an integrated inertial and magnetophoretic device
, Article Chemical Engineering Science ; Volume 252 , 2022 ; 00092509 (ISSN) ; Shamloo, A ; Akbari, J ; Sharif University of Technology
Elsevier Ltd
2022
Abstract
The separation of cancer cells from a heterogeneous biological sample such as blood plays a vital role in cancer study and future treatments. In this paper, we designed and investigated two microfluidic devices for cancer cell separation, including a serpentine inertial device and an integrated inertial-magnetophoretic device. Firstly, numerical modeling was carried out to study the fluid flow, particles’ trajectories in the inertial device. Then the device was fabricated using soft photolithography and suspension of two types of microparticles with the size of 10 and 15 µm were injected into the microchannel separately to investigate the particles’ trajectories and focusing behavior at...
Integrating hydrodynamic and acoustic cell separation in a hybrid microfluidic device: a numerical analysis
, Article Acta Mechanica ; Volume 233, Issue 5 , 2022 , Pages 1881-1894 ; 00015970 (ISSN) ; Dizani, M ; Shamloo, A ; Sharif University of Technology
Springer
2022
Abstract
Cell separation microfluidic devices have evolved into a multitude of biomedical and clinical research. Nonetheless, many critical issues remain in the way of achieving an excellent separation of target cells from a heterogeneous sample. Parallel to the abundant experimental studies related to the hybrid microfluidic methods, it is easy to perceive the lack of numerical investigations in order to optimize the separation process and its accuracy. In this study, for the first time to the best of our knowledge, a hybrid system by integrating acoustophoresis and pinched-flow fractionation (PFF) is proposed to achieve a viable system for a wide-range, precise separation. Employing the ultrasound...
Brain-on-a-chip: Recent advances in design and techniques for microfluidic models of the brain in health and disease
, Article Biomaterials ; Volume 285 , 2022 ; 01429612 (ISSN) ; Shamloo, A ; Nasiri, R ; de Barros, N. R ; Wang, Z. Z ; Unluturk, B. D ; Libanori, A ; Ievglevskyi, O ; Diltemiz, S. E ; Sances, S ; Balasingham, I ; Seidlits, S. K ; Ashammakhi, N ; Sharif University of Technology
Elsevier Ltd
2022
Abstract
Recent advances in biomaterials, microfabrication, microfluidics, and cell biology have led to the development of organ-on-a-chip devices that can reproduce key functions of various organs. Such platforms promise to provide novel insights into various physiological events, including mechanisms of disease, and evaluate the effects of external interventions, such as drug administration. The neuroscience field is expected to benefit greatly from these innovative tools. Conventional ex vivo studies of the nervous system have been limited by the inability of cell culture to adequately mimic in vivo physiology. While animal models can be used, their relevance to human physiology is uncertain and...
Design of the micropump and mass-transfer compartment of a microfluidic system for regular nonenzymatic glucose measurement
, Article Biotechnology Reports ; Volume 34 , 2022 ; 2215017X (ISSN) ; Saidi, M. S ; Kazemzadeh Hannani, S ; Sharif University of Technology
Elsevier B.V
2022
Abstract
The aim of this paper is to design and numerically simulate the mass-transfer compartment and piezoelectric micropump of an implantable integrated microfluidic device for regular microdialysis-based nonenzymatic measurement of glucose level in diabetic patients. The device function is based on the process that the piezoelectric micropump pumps the dialysis fluid into the mass-transfer compartment microchannels, where the interstitial fluid (ISF) glucose diffusion into this dialysis fluid gives it a glucose content, then detected and measured in the sensor section. This diffusion takes place through the semipermeable membranes located in the microchannels at the base of the hollow...