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MiR-361-5p as a promising qRT-PCR internal control for tumor and normal breast tissues
, Article PLoS ONE ; Volume 16, Issue 6 June , 2021 ; 19326203 (ISSN) ; Salimi, A ; Rahmani, S ; Nafissi, N ; Sharifi Zarchi, A ; Mowla, S. J ; Sharif University of Technology
Public Library of Science
2021
Abstract
Background: One of the most widely used evaluation methods in miRNA experiments is qRT-PCR. However, selecting suitable internal controls (IC) is crucial for qRT-PCR experiments. Currently, there is no consensus on the ICs for miRNA qRT-PCR experiments in breast cancer. To this end, we tried to identify the most stable (the least expression alteration) and promising miRNAs in normal and tumor breast tissues by employing TCGA miRNA-Seq data and then experimentally validated them on fresh clinical samples. Methods: A multi-component scoring system was used which takes into account multiple expression stability criteria as well as correlation with clinical characteristics. Furthermore, we...