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    Delivery of melittin-loaded niosomes for breast cancer treatment: an in vitro and in vivo evaluation of anti-cancer effect

    , Article Cancer Nanotechnology ; Volume 12, Issue 1 , 2021 ; 18686958 (ISSN) Dabbagh Moghaddam, F ; Akbarzadeh, I ; Marzbankia, E ; Farid, M ; khaledi, L ; Reihani, A. H ; Javidfar, M ; Mortazavi, P ; Sharif University of Technology
    BioMed Central Ltd  2021
    Background: Melittin, a peptide component of honey bee venom, is an appealing candidate for cancer therapy. In the current study, melittin, melittin-loaded niosome, and empty niosome had been optimized and the anticancer effect assessed in vitro on 4T1 and SKBR3 breast cell lines and in vivo on BALB/C inbred mice. "Thin-layer hydration method" was used for preparing the niosomes; different niosomal formulations of melittin were prepared and characterized in terms of morphology, size, polydispersity index, encapsulation efficiency, release kinetics, and stability. A niosome was formulated and loaded with melittin as a promising drug carrier system for chemotherapy of the breast cancer cells.... 

    Can the body slope of interference screw affect initial stability of reconstructed anterior cruciate ligament?: An in-vitro investigation

    , Article BMC Musculoskeletal Disorders ; Volume 22, Issue 1 , 2021 ; 14712474 (ISSN) Daneshvarhashjin, N ; Chizari, M ; Mortazavi, J ; Rouhi, G ; Sharif University of Technology
    BioMed Central Ltd  2021
    Background: Superior biomechanical performance of tapered interference screws, compared with non-tapered screws, with reference to the anterior cruciate ligament (ACL) reconstruction process, has been reported in the literature. However, the effect of tapered interference screw’s body slope on the initial stability of ACL is poorly understood. Thus, the main goal of this study was to investigate the effect of the interference screw’s body slope on the initial stability of the reconstructed ACL. Methods: Based on the best screw-bone tunnel diameter ratios in non-tapered screws, two different tapered interference screws were designed and fabricated. The diameters of both screws were equal to... 

    Homozygous mutations in C14orf39/SIX6OS1 cause non-obstructive azoospermia and premature ovarian insufficiency in humans

    , Article American Journal of Human Genetics ; Volume 108, Issue 2 , 2021 , Pages 324-336 ; 00029297 (ISSN) Fan, S ; Jiao, Y ; Khan, R ; Jiang, X ; Javed, A. R ; Ali, A ; Zhang, H ; Zhou, J ; Naeem, M ; Murtaza, G ; Li, Y ; Yang, G ; Zaman, Q ; Zubair, M ; Guan, H ; Zhang, X ; Ma, H ; Jiang, H ; Ali, H ; Dil, S ; Shah, W ; Ahmad, N ; Zhang, Y ; Shi, Q ; Sharif University of Technology
    Cell Press  2021
    Human infertility is a multifactorial disease that affects 8%–12% of reproductive-aged couples worldwide. However, the genetic causes of human infertility are still poorly understood. Synaptonemal complex (SC) is a conserved tripartite structure that holds homologous chromosomes together and plays an indispensable role in the meiotic progression. Here, we identified three homozygous mutations in the SC coding gene C14orf39/SIX6OS1 in infertile individuals from different ethnic populations by whole-exome sequencing (WES). These mutations include a frameshift mutation (c.204_205del [p.His68Glnfs∗2]) from a consanguineous Pakistani family with two males suffering from non-obstructive... 

    Biological removal of nutrients (N & P) from urban wastewater with a modified integrated fixed-film activated sludge-oxic settling anoxic system using an anoxic sludge holding tank

    , Article Water and Environment Journal ; Volume 35, Issue 2 , 2021 , Pages 830-846 ; 17476585 (ISSN) Fazelipour, M ; Takdastan, A ; Borghei, S. M ; Sharif University of Technology
    John Wiley and Sons Inc  2021
    In this research, the efficiency of the integrated fixed-film activated sludge-oxic settling anoxic (IFAS-OSA) system in biological nutrient removal was studied. The oxic-settling anoxic (OSA) process is known as a cost effective way to reduce the nutrients (nitrogen and Phosphorus). According to the results, the percentages of total nitrogen removal efficiency in the IFAS, IFAS-OSA2h and IFAS-OSA4h systems were 78.56 ± 2.46, 83.60 ± 0.92 and 85.03 ± 1.69, respectively, while the percentages of phosphorus removal efficiency in these systems were 32.69 ± 8.25, 36.35 ± 6.73 and 39.87 ± 3.61, respectively. The PCR-RFLP method showed that C. albicans had the greatest prevalence (n = 36, 90%).... 

    Common coding of expected value and value uncertainty memories in the prefrontal cortex and basal ganglia output

    , Article Science Advances ; Volume 7, Issue 20 , 2021 ; 23752548 (ISSN) Ghazizadeh, A ; Hikosaka, O ; Sharif University of Technology
    American Association for the Advancement of Science  2021
    Recent evidence implicates both basal ganglia and ventrolateral prefrontal cortex (vlPFC) in encoding value memories. However, comparative roles of cortical and basal nodes in value memory are not well understood. Here, single-unit recordings in vlPFC and substantia nigra reticulata (SNr), within macaque monkeys, revealed a larger value signal in SNr that was nevertheless correlated with and had a comparable onset to the vlPFC value signal. The value signal was maintained for many objects (>90) many weeks after reward learning and was resistant to extinction in both regions and to repetition suppression in vlPFC. Both regions showed comparable granularity in encoding expected value and value... 

    Rhythmic air-puff into nasal cavity modulates activity across multiple brain areas: A non-invasive brain stimulation method to reduce ventilator-induced memory impairment

    , Article Respiratory Physiology and Neurobiology ; Volume 287 , 2021 ; 15699048 (ISSN) Ghazvineh, S ; Salimi, M ; Nazari, M ; Garousi, M ; Tabasi, F ; Dehdar, K ; Salimi, A ; Jamaati, H ; Mirnajafi Zadeh, J ; Arabzadeh, E ; Raoufy, M. R ; Sharif University of Technology
    Elsevier B.V  2021
    Mechanical ventilation (MV) can result in long-term brain impairments that are resistant to treatment. The mechanisms underlying MV-induced brain function impairment remain unclear. Since nasal airflow modulates brain activity, here we evaluated whether reinstating airflow during MV could influence the memory performance of rats after recovery. Rats were allocated into two study groups: one group received rhythmic air-puff into the nasal cavity during MV and a control group that underwent ventilation without air-puff. During MV, air-puffs induced time-locked event potentials in OB, mPFC and vHPC and significantly increased the oscillatory activity at the air-puff frequency. Furthermore, in... 

    Healthy and diseasedin vitromodels of vascular systems

    , Article Lab on a Chip ; Volume 21, Issue 4 , 2021 , Pages 641-659 ; 14730197 (ISSN) Hosseini, V ; Mallone, A ; Nasrollahi, F ; Ostrovidov, S ; Nasiri, R ; Mahmoodi, M ; Haghniaz, R ; Baidya, A ; Salek, M. M ; Darabi, M. A ; Orive, G ; Shamloo, A ; Dokmeci, M. R ; Ahadian, S ; Khademhosseini, A ; Sharif University of Technology
    Royal Society of Chemistry  2021
    Irregular hemodynamics affects the progression of various vascular diseases, such atherosclerosis or aneurysms. Despite the extensive hemodynamics studies on animal models, the inter-species differences between humans and animals hamper the translation of such findings. Recent advances in vascular tissue engineering and the suitability ofin vitromodels for interim analysis have increased the use ofin vitrohuman vascular tissue models. Although the effect of flow on endothelial cell (EC) pathophysiology and EC-flow interactions have been vastly studied in two-dimensional systems, they cannot be used to understand the effect of other micro- and macro-environmental parameters associated with... 

    Preparation and evaluation of various banana-based biochars together with ultra-high performance liquid chromatography-tandem mass spectrometry for determination of diverse pesticides in fruiting vegetables

    , Article Food Chemistry ; Volume 360 , 2021 ; 03088146 (ISSN) Keikavousi Behbahan, A ; Mahdavi, V ; Roustaei, Z ; Bagheri, H ; Sharif University of Technology
    Elsevier Ltd  2021
    Biomass, originates from plant- or animal-based materials with a huge potential to be reused. Here we report a simple, rapid and inexpensive method for preparation of modified biochars derived from the banana peel followed by their applications in pipette-tip micro solid-phase extraction (PT-µSPE). Due to the contribution of various effective parameters on modification of banana peel biochars (BPBs), Taguchi design was used to optimize activation temperature, activation repetition, treatment material and impregnation ratio. Efficiency of the prepared BPBs were studied by extraction of twelve various pesticides, as model analytes with an extended range of log P values (1.4–5.7), followed by... 

    Improved green biosynthesis of chitosan decorated Ag- and Co3O4-nanoparticles: A relationship between surface morphology, photocatalytic and biomedical applications

    , Article Nanomedicine: Nanotechnology, Biology, and Medicine ; Volume 32 , 2021 ; 15499634 (ISSN) Kiani, M ; Rabiee, N ; Bagherzadeh, M ; Ghadiri, A.M ; Fatahi, Y ; Dinarvand, R ; Webster, T. J ; Sharif University of Technology
    Elsevier Inc  2021
    AgNPs@Chitosan and Co3O4-NPs@Chitosan were fabricated with Salvia hispanica. Results showed MZI values of 5 and 30 mm for Co3O4-NPs- and AgNPs@Chitosan against S. aureus, and 15 and 21 mm for Co3O4-NPs- and AgNPs@Chitosan against E. coli (24 h, 20 μg/mL), respectively. MTT assays showed up to 80% and 90%, 71% and 75%, and 91% and 94% mammalian cell viability for the green synthesized, chemically synthesized AgNPs and green synthesized AgNPs@Chitosan for HEK-293 and PC12 cells, respectively, and 70% and 71%, 59% and 62%, and 88% and 73% for the related Co3O4-NPs (24 h, 20 μg/mL). The photocatalytic activities showed dye degradation after 135 and 105 min for AgNPs@Chitosan and... 

    An innovative, highly stable Ag/ZIF-67@GO nanocomposite with exceptional peroxymonosulfate (PMS) activation efficacy, for the destruction of chemical and microbiological contaminants under visible light

    , Article Journal of Hazardous Materials ; Volume 413 , 2021 ; 03043894 (ISSN) Kohantorabi, M ; Giannakis, S ; Moussavi, G ; Bensimon, M ; Gholami, M. R ; Pulgarin, C ; Sharif University of Technology
    Elsevier B.V  2021
    In this work, Ag nanoparticles were loaded on ZIF-67 covered by graphene oxide (Ag/ZIF-67@GO), and its catalytic performance was studied for the heterogeneous activation of peroxymonosulfate (PMS) under visible-light. The catalyst surface morphology and structure were analyzed by FT-IR, XRD, XPS, DRS, FE-SEM, EDX, TEM, BET, ICP-AES and TGA analysis. The efficacy of PMS activation by the Ag/ZIF-67@GO under visible light was assessed by phenol degradation and E. coli inactivation. Phenol was completely degraded within 30 min by HO•, SO4•− and O2•− generated through the photocatalytic PMS activation. In addition, total E. coli inactivation was attained in 15 min that confirmed the highly... 

    Simultaneous detection and identification of thiometon, phosalone, and prothioconazole pesticides using a nanoplasmonic sensor array

    , Article Food and Chemical Toxicology ; Volume 151 , 2021 ; 02786915 (ISSN) Koushkestani, M ; Abbasi Moayed, S ; Ghasemi, F ; Mahdavi, V ; Hormozi Nezhad, M. R ; Sharif University of Technology
    Elsevier Ltd  2021
    In this work, a colorimetric sensor array has been designed for the identification and discrimination of thiometon (TM) and phosalone (PS) as organophosphate pesticides and prothioconazole (PC) as a triazole pesticide. For this purpose, two different plasmonic nanoparticles including unmodified gold nanoparticles (AuNPs) and unmodified silver nanoparticles (AgNPs) were used as sensing elements. The principle of the proposed strategy relied on the aggregation AuNPs and AgNPs through the cross-reactive interaction between the target pesticides and plasmonic nanoparticles. Therefore, these aggregation-induced UV–Vis spectra changes were utilized to discriminate the target pesticides with the... 

    Targeted nanomedicines for the treatment of bone disease and regeneration

    , Article Medicinal Research Reviews ; Volume 41, Issue 3 , 2021 , Pages 1221-1254 ; 01986325 (ISSN) Ordikhani, F ; Zandi, N ; Mazaheri, M ; Luther, G. A ; Ghovvati, M ; Akbarzadeh, A ; Annabi, N ; Sharif University of Technology
    John Wiley and Sons Inc  2021
    Targeted delivery by either passive or active targeting of therapeutics to the bone is an attractive treatment for various bone related diseases such as osteoporosis, osteosarcoma, multiple myeloma, and metastatic bone tumors. Engineering novel drug delivery carriers can increase therapeutic efficacy and minimize the risk of side effects. Developmnet of nanocarrier delivery systems is an interesting field of ongoing studies with opportunities to provide more effective therapies. In addition, preclinical nanomedicine research can open new opportunities for preclinical bone-targeted drug delivery; nevertheless, further research is needed to progress these therapies towards clinical... 

    PLGA/TiO2 nanocomposite scaffolds for biomedical applications: Fabrication, photocatalytic, and antibacterial properties

    , Article BioImpacts ; Volume 11, Issue 1 , 2021 , Pages 45-52 ; 22285652 (ISSN) Pelaseyed, S. S ; Madaah Hosseini, H. R ; Nokhbedehghan, Z ; Samadikuchaksaraei, A ; Sharif University of Technology
    Tabriz University of Medical Sciences  2021
    Introduction: Porous 3D scaffolds synthesized using biocompatible and biodegradable materials could provide suitable microenvironment and mechanical support for optimal cell growth and function. The effect of the scaffold porosity on the mechanical properties, as well as the TiO2 nanoparticles addition on the bioactivity, antimicrobial, photocatalytic, and cytotoxicity properties of scaffolds were investigated. Methods: In the present study, porous scaffolds consisting poly (lactide-co-glycolide) (PLGA) containing TiO2 nanoparticles were fabricated via air-liquid foaming technique, which is a novel method and has more advantages due to not using additives for nucleation compared to former... 

    Diatoms with invaluable applications in nanotechnology, biotechnology, and biomedicine: Recent advances

    , Article ACS Biomaterials Science and Engineering ; Volume 7, Issue 7 , 2021 , Pages 3053-3068 ; 23739878 (ISSN) Rabiee, N ; Khatami, M ; Jamalipour Soufi, G ; Fatahi, Y ; Iravani, S ; Varma, R. S ; Sharif University of Technology
    American Chemical Society  2021
    Diatoms are unicellular microalga found in soil and almost every aquatic environment (marine and fresh water). Biogenic silica and diatoms are attractive for biotechnological and industrial applications, especially in the field of biomedicine, industrial/synthetic manufacturing processes, and biomedical/pharmaceutical sciences. Deposition of silica by diatoms allows them to create micro- or nanoscale structures which may be utilized in nanomedicine and especially in drug/gene delivery. Diatoms with their unique architectures, good thermal stability, suitable surface area, simple chemical functionalization/modification procedures, ease of genetic manipulations, optical/photonic... 

    Polymeric nanoparticles for nasal drug delivery to the brain: relevance to alzheimer's disease

    , Article Advanced Therapeutics ; Volume 4, Issue 3 , 2021 ; 23663987 (ISSN) Rabiee, N ; Ahmadi, S ; Afshari, R ; Khalaji, S ; Rabiee, M ; Bagherzadeh, M ; Fatahi, Y ; Dinarvand, R ; Tahriri, M ; Tayebi, L ; Hamblin, M. R ; Webster, T. J ; Sharif University of Technology
    Blackwell Publishing Ltd  2021
    Currently, Alzheimer's disease (AD) accounts for more than half of all dementia cases. Although genetics, age, and environmental factors affect the disease, the cause of AD is not yet fully known. Various drugs have been proposed for the prevention and treatment of AD, but the delivery of these therapeutic agents to the brain is difficult. The blood–brain barrier prevents systemic drugs from accessing the central nervous system and designing a suitable system to overcome this barrier has attracted much attention. The intranasal pathway, given its proximity to the brain, provides a great opportunity for drug delivery. Understanding the physiological characteristics of the nose can be useful... 

    Nanotechnology-assisted microfluidic systems: From bench to bedside

    , Article Nanomedicine ; Volume 16, Issue 3 , 2021 , Pages 237-258 ; 17435889 (ISSN) Rabiee, N ; Ahmadi, S ; Fatahi, Y ; Rabiee, M ; Bagherzadeh, M ; Dinarvand, R ; Bagheri, B ; Zarrintaj, P ; Saeb, M. R ; Webster, T. J ; Sharif University of Technology
    Future Medicine Ltd  2021
    With significant advancements in research technologies, and an increasing global population, microfluidic and nanofluidic systems (such as point-of-care, lab-on-a-chip, organ-on-a-chip, etc) have started to revolutionize medicine. Devices that combine micron and nanotechnologies have increased sensitivity, precision and versatility for numerous medical applications. However, while there has been extensive research on microfluidic and nanofluidic systems, very few have experienced wide-spread commercialization which is puzzling and deserves our collective attention. For the above reasons, in this article, we review research advances that combine micro and nanotechnologies to create the next... 

    Dna-Rna hybrid (R-loop): From a unified picture of the mammalian telomere to the genome-wide profile

    , Article Cells ; Volume 10, Issue 6 , 2021 ; 20734409 (ISSN) Rassoulzadegan, M ; Sharifi Zarchi, A ; Kianmehr, L ; Sharif University of Technology
    MDPI  2021
    Local three-stranded DNA/RNA hybrid regions of genomes (R-loops) have been detected either by binding of a monoclonal antibody (DRIP assay) or by enzymatic recognition by RNaseH. Such a structure has been postulated for mouse and human telomeres, clearly suggested by the identification of the complementary RNA Telomeric repeat-containing RNA “TERRA”. However, the tremendous disparity in the information obtained with antibody-based technology drove us to investigate a new strategy. Based on the observation that DNA/RNA hybrids in a triplex complex genome co-purify with the double-stranded chromosomal DNA fraction, we developed a direct preparative approach from total protein-free cellular... 

    Estimation of nitrogen content in cucumber plant (Cucumis sativus L.) leaves using hyperspectral imaging data with neural network and partial least squares regressions

    , Article Chemometrics and Intelligent Laboratory Systems ; Volume 217 , 2021 ; 01697439 (ISSN) Sabzi, S ; Pourdarbani, R ; Rohban, M. H ; García Mateos, G ; Arribas, J. I ; Sharif University of Technology
    Elsevier B.V  2021
    In recent years, farmers have often mistakenly resorted to overuse of chemical fertilizers to increase crop yield. However, excessive consumption of fertilizers might lead to severe food poisoning. If nutritional deficiencies are detected early, it can help farmers to design better fertigation practices before the problem becomes unsolvable. The aim of this study is to predict the amount of nitrogen (N) content (mg l−1) in cucumber (Cucumis sativus L., var. Super Arshiya-F1) plant leaves using hyperspectral imaging (HSI) techniques and three different regression methods: a hybrid artificial neural networks-particle swarm optimization (ANN-PSO); partial least squares regression (PLSR); and... 

    The olfactory bulb modulates entorhinal cortex oscillations during spatial working memory

    , Article Journal of Physiological Sciences ; Volume 71, Issue 1 , 2021 ; 18806546 (ISSN) Salimi, M ; Tabasi, F ; Nazari, M ; Ghazvineh, S ; Salimi, A ; Jamaati, H ; Raoufy, M. R ; Sharif University of Technology
    BioMed Central Ltd  2021
    Cognitive functions such as working memory require integrated activity among different brain regions. Notably, entorhinal cortex (EC) activity is associated with the successful working memory task. Olfactory bulb (OB) oscillations are known as rhythms that modulate rhythmic activity in widespread brain regions during cognitive tasks. Since the OB is structurally connected to the EC, we hypothesized that OB could modulate EC activity during working memory performance. Herein, we explored OB–EC functional connectivity during spatial working memory performance by simultaneous recording local field potentials when rats performed a Y-maze task. Our results showed that the coherence of delta,... 

    Allergic rhinitis impairs working memory in association with drop of hippocampal – Prefrontal coupling

    , Article Brain Research ; Volume 1758 , 2021 ; 00068993 (ISSN) Salimi, M ; Ghazvineh, S ; Nazari, M ; Dehdar, K ; Garousi, M ; Zare, M ; Tabasi, F ; Jamaati, H ; Salimi, A ; Barkley, V ; Mirnajafi Zadeh, J ; Raoufy, M. R ; Sharif University of Technology
    Elsevier B.V  2021
    Allergic rhinitis (AR) is a chronic inflammatory disease frequently associated with a deficit in learning and memory. Working memory is an important system for decision making and guidance, which depends on interactions between the ventral hippocampus (vHipp) and the prelimbic prefrontal cortex (plPFC). It is still unclear whether AR influences the activity and coupling of these brain areas, which consequently may impair working memory. The current study aimed to examine alterations of the vHipp-plPFC circuit in a rat model of AR. Our results show decreased working memory performance in AR animals, accompanied by a reduction of theta and gamma oscillations in plPFC. Also, AR reduces...