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Quantitative in vivo microsampling for pharmacokinetic studies based on an integrated solid-phase microextraction system
, Article Analytical Chemistry ; Volume 79, Issue 12 , 2007 , Pages 4507-4513 ; 00032700 (ISSN) ; Eshaghi, A ; Musteata, F. M ; Ouyang, G ; Pawliszyn, J ; Sharif University of Technology
2007
Abstract
An integrated microsampling approach based on solid-phase microextraction (SPME) was developed to provide a complete solution to highly efficient and accurate pharmacokinetic studies. The microsampling system included SPME probes that are made of poly(ethylene glycol) (PEG) and C18-bonded silica, a fast and efficient sampling strategy with accurate kinetic calibration, and a high-throughput desorption device based on a modified 96-well plate. The sampling system greatly improved the quantitative capability of SPME in two ways. First, the use of the C18-bonded silica/PEG fibers minimized the competition effect from analogues of the target analytes in a complicated sample matrix such as blood...