Sharif Digital Repository

Asparagainase is a therapeutic enzyme which has been a subject of research for decades. The enzyme catalyzes the hydrolysis of the amide group in asparagine and similar amides. Altering the substrate specificity and stabilization of this enzyme can increase its therapeutic properties. Moreover, asparaginases may be evolved to catalyze the hydrolysis of other similar compounds. These can be achieved through directed evolution and computational methods.In this study, the gene encoding L-asparaginase II enzyme from E. coli was amplified by polymerase chain reaction (PCR) and was cloned into an expression vector. The recombinant protein was expressed by an appropriate host secreting the... 

Thin films of materials, in order to have magnificent structure and properties, can use in complicated technology such as; electronic, optic and magnetic systems. Printed interconnects can be made by the deposition of suspensions of metal nanoparticles (e.g., silver and gold) followed by thermal sintering. The nanoparticle sintering process involves atomic diffusion resulting in shrinkage. As a result, the interconnects are prone to residual stresses generated during the thermal sintering process. The residual stress can cause cracks and delamination in the thin films and can cause permanent geometrical changes in the underlying substrates which may affect the functionality of the... 

The commercial breakthrough of micro-tubular solid oxide fuel cells (MT-SOFCs) is still facing so many challenges because of the complexity of their manufacturing process and the high cost associated with them. By deploying electrophoretic deposition as a simple and cost-effective method, the different components of the fuel cells (the anode, electrolyte, and cathode) can deposit on the graphite rod as a sacrificial substrate. The next step is eliminating the sacrificial substrate which will make hollow rods of cell components. The results of this study show that the use of a particular type of pencil lead will have a minimal negative impact on the overall fuel cell performance, leading to a... 

Mesoscopic hydrodynamic equations are solved employing a VOF based method to investigate the equilibrium shape of nanodroplets positioned over various topographic geometries of the supporting substrate for three-dimensional systems. By taking into account liquid-liquid and liquid-solid interactions a complex distribution for inter-molecular forces over the substrates (the disjoining pressure) is observed. In this research we show that motion of nanodroplets not only caused by contact angle difference in drplets two sides, but also depend on disjoining pressure parameters.Geometries with increasing complexities, from wedges to three dimensional edges and wedges, were explored with the main... 

L-asparaginase II is an amidohydrolase which hydrolyzes L-asparagine to furnish L-aspartic acid and ammonia. The enzyme has played an important role for 40 years in treating cancer in persons with juvenile leukemias or lymphomas. Furthermore, the enzyme has become important in food industries and biosensors technology.In this study, the expression of recombinant L-asparaginase II in E. Coli was optimized and the purification of this enzyme from growth culture was carried out with nickel-charged affinity resin. Afterwards, the activity, and kinetic parameters of purified recombinant L-asparaginase II including Km, kcat, Vmax, and enzyme efficiency were measured.In the next step, random... 

A microbial fuel cell (MFC) is a bioreactor that utilizes chemical energy in the chemical bonds of organic compounds to electrical energy production through catalytic reactions of microorganisms under anaerobic conditions. In this study, the performance of a dual chamber, mediator-less anode, graphite electrodes MFC employing mixed consortia as anodic inoculums and using a combination of formalin and glucose as substrate was investigated. Different MFCs with different concentration of formalin and fixed concentration of glucose was operated to elucidate the difference in power generation and removal of formalin as an industrial pollutant under similar operating conditions [ambient pressure;... 

A droplet placed on the boundary of two solids with different wettabilities will move to the more wettable part. This is a well-known phenomenon and has been extensively used in a variety of processes and applications ranging from biological systems and ink jet printing to the commercial lab-on-a-chip. Because of its importance, many studies are conducted around this phenomenon. The difference between the equilibrium contact angles of the droplet on the two parts gives rise to an interfacial driving force which moves the droplet. Such a motion is not continuous as the droplet slides to the more wettable area, the driving force diminishes and consequently the droplet stops. A non-stop motion... 

Hydroxyapatite (HA) is an attractive biomaterial due to its close chemical composition to bone and teeth. However, HA is brittle and has low strength under bearing loads, resulting in limition for orthopedic and dental applications. Two creative approaches have been developed to overcome this problem, using bioactive HA as coatings on metallic implants or as ceramic/metal composites. Carbon nanotubes (CNT) possess excellent mechanical properties to play the role as reinforcement for imparting strength and toughness to brittle hydroxyapatite (HA) bioceramic coating. Moreover, The addition of TiO2 to HA coatings has attracted considerable attention, since TiO2 is capable of increasing the... 

Hydrodynamic mesoscopic equations are solved using boundary integral method to investigate the coarsening dynamics of two dimensional nanodroplets. The dynamics is probed by locating two drops on homogeneous, chemical heterogeneous and physical heterogeneous substrates respectively. For homogeneous substrate, the effect of different parameters like droplets distance, slip boundary condition, surface contact angle and disjoining pressure type on the dynamics is studied. Results reveal that increasing the contact angles to values larger than a critical value may qualitatively change the coarsening process and the profile of the disjoining pressure can appreciably modify the coarsening rate.... 

In this thesis a substrate integrated waveguide (SIW) filter is designed and constructed using the contour integral method. A substrate integrated waveguide can overcome the drawbacks of regular waveguides, namely bulkiness, high costs and the difficulty to be integrated with other elements in high frequencies. In planar structures with arbitrary shapes where green’s functions aren’t available, we can use the contour integral method to construct the impedance matrix. Because the SIW structure supports TEn0¬ modes, we can consider SIW as a planar structure and thus use the contour integral method. Regular filter structures are usually analyzed using the FEM method (with HFSS) or the FIT... 

Graphene is a single-atomic two-dimensional crystal that has considerable properties due to its unique structure and physics with applications in different fields. Due to its atomic thin thickness, substrates have a considerable effect on the electrical properties of Graphene. As these effects are determinative of the general behavior of systems, one should consider this effect in the design and fabrication of a device. Moreover, the conventional fabrication methods for Graphene-based electronic devices restrict the possibility of the device fabrication on a wide range of substrates, specially flexible and biocompatible substrates.In this work, the LPCVD Graphene growth process has been... 

In the recent years, nanophotocatalytic process for E. coli inactivation has become considerable choice for water treatment. Having suitable catalytic characterizations, nanophotocatalysis could be considered as an alternative to current E. coli disinfections methods.
In this study, antibacterial effect of Fe-Cd/TiO2 (TiO2 doped with Fe and Cd) under visible light was reported for the first time. The Results show that by comparing Fe-Cd/TiO2 with commercial TiO2 (P25 Degussa), bactericidal efficiency of this codoped photocatalysis under visible light is high and effective. Indeed, Nanoparticle of Fe-Cd/TiO2 disinfects E. coli (cell density: 107 CFU/ml) almost completely within 55 min... 

Millimeter-wave imaging is used in a vast applications like security, medical and non-destrcutive testings. Frequency scanning antenna is one of the optimum solution in mm-wave imaging systems, because of its accuracy, agility and low cost. In this method, changing the frequency plays the main role and leads the main beam to scan the space. In this thesis, by observing different frequency scanning structures, like microstrip antenna, slotted waveguide antenna array, substrate integrated waveguide leaky-wave antenna, different structures are designed and simulated. The antenna, which results in the best performance for an imaging system is selected for fabrication and measurement. The... 

Novel Dirac Leaky Wave Antennas (DLWAs) based on Substrate Integrated Waveguide (SIW) are presented in this thesis, for millimeter wave frequencies. These DLWAs provide interesting features such as radiating through broadside, beam scanning and ease in fabrication that are well suited for emerging fifth generation (5G) and IoT applications. In this thesis, it is shown that a Dirac photonic crystal can be realized in SIW technology composed of air columns inside a host SIW waveguide, exhibiting a closed bandgap and linear dispersion around broadside. Phase and attenuation constants are controlled to obtain directive beam and scanning in a wide range of angles (from -30˚ to 20˚). This DLWA... 

In order to prevent electrostatic attraction on non-conductive surfaces, the most practical solution is to put antistatic coatings to reduce surface resistance. Nano tin oxide coatings are designed to produce surfaces with antistatic properties in the resistance range of 108-1012 Ω/cm. In this project, using a simple method, low temperature, without heat treatment and without the use of additive (dopant), tin oxide films with antistatic property were placed on the glass. Further, in order to obtain a coating with a suitable morphology and structure to increase the surface conductivity, the synthesis of tin oxide using the sol-gel method at ambient temperature was used. And to place the... 

In this research, sliding contact problem of a rounded apex wedge with a FG coated substrate is investigated. The structure includes two components. The first is the FG coating and then, the homogeneous substrate which is connected to the FG coating. Usually, it is considered that the homogeneous substrate is a metal and properties at the connection are metallic. Then, properties varies gradually through the thickness of the FG coating, so, the material at the surface of the structure is a ceramic. It is assumed that, properties varies exponentially and continuously through the FG coating and the poisson’s ratio is constant. Beside, it is supposed that the conditions of coulomb friction... 

The Cu2ZnSnS4 (CZTS) quaternary compound, with suitable optical and electrical properties, is one of the most interesting materials for absorber layer of thin film solar cell. Since all constituents of CZTS, are abundant in earth’s crust and non-toxic, it is a proper replacement for the materials like CdTe and CuInSe2. The CZTS pellet was synthesized by the solid-state reaction and deposited on soda lime glass substrates by pulsed laser deposition (PLD) method. PLD technique has the advantages of offering stoichiometric preservation during the materials transformation from target to substrate and good crystallinity due to the highly energetic species. CZTS has was deposited in order to... 

In this thesis, the improving effects of deposited metal oxides onto or next to each other and the advantages of every metal oxide were considered. In the next step, the substrate improvement was studied and the porous metal oxides with ideal supercapacitive properties with porosity increase of substrate were obtained.CoFeO2 supercapacitor was prepared by chronoamperometric ethod.CoFeO2waselectrodeposited at room temperature and presents wide potential window and good capacitive properties in comparison with other metal oxides.Layers of cobalt and manganese oxides were co-deposited or deposited on top of each other or next to each other by chronometric method onto stainless steel substrate.... 

In this thesis, Silicon nanowires were synthesized using carbothermal method via Vapor-Liquid-Solid mechanism catalyzed by gold nanoparticles deposited on various substrates. Silicon nanowires just grew on (100) and (111)-oriented silicon substrates. For a particular set of process parameters, we observed a critical thickness of the nucleating gold film, that had depened on a kind of various substrates. We studied the dependence of the Au-Si alloy droplet size and size distribution on the starting gold film thickness and the annealing conditions. We used a modified heating sequence that deconvoluted the effect of various substrates consumption and gas-phase silicon supply on the Au-Si alloy... 

In the recent years, there has been great interest in exploring methods for assembly and manipulation at the micro/nanoscale to build miniaturized systems, devices, structures, and machines. This thesis aims at two-dimensional manipulation of nanoparticle using Atomic Force Microscope (AFM) probe. The nanoprobe is used to push the spherical micro/nanoparticle. Continuum based modeling and simulation of the manipulation task is presented. The proposed nanomanipulation model consists of all effective phenomena in nanoscale. Nanoscale interaction forces, elastic deformation in contact areas, and friction forces in tip/nanoparticle/substrate system are considered. The utilized friction models...