Sharif Digital Repository

In recent years, applications of dielectrophoresis-based platforms have been recognized as effective and dependable approach to separate cells and bioparticles, suspended in different carrier fluids, based on particle size and electrical properties. In this study, a microfluidic device was fabricated by an unprecedented electrode pattern, and several experiments were performed to enrich samples including either of yeast, Escherichia coli, or latex particles. A chemical deposition-based method was employed for fabrication of microelectrodes, inducing nonuniform electric field required for dielectrophoresis-based separation. One major advantage of our employed method is low fabrication cost,... 

Although curcumin is highly cytotoxic against cancer cells, its hydrophobicity and fast degradation at physiological pH limit its effective practical application. To prevent such limitations, inexpensive curcumin-loaded starch particles are synthesized in this research. Particles are prepared by water-in-oil (W/O) miniemulsion technique and an adsorption method is used for curcumin loading. Also, encapsulation efficiency (%EE) is improved by using pluronic F-127 in the drug solution. Particles are characterized, swelling studies are performed, and MTT assays against human adipose mesenchymal stem cells (hAMSCs) and MG-63 cells are utilized for investigations. Results indicate that... 

Nowadays, putting forward an accurate cancer therapy method with minimal side effects is an important topic of research. Nanostructures, for their ability in controlled and targeted drug release on specific cells, are critical materials in this field. In this study, a pH-sensitive graphene oxide-l-arginine nanogel was synthesized to carry and release 5-fluorouracil. Optimized conditions using statistical analysis, based on the maximum relative viscosity of nanogel, were evaluated: 5.489 for the concentration of l-arginine and 2.404 for pH. The prepared nanogels were characterized using scanning electron microscope and transmission electron microscope images and Fourier-transform infrared... 

Sulfur compounds are essential for many industries and organisms; however, they cause serious respiratory problems in human beings. Therefore, determination of sulfur concentration is of paramount importance. The research approach in the field of detecting contaminants has led to smaller systems that provide faster and more effective ways for diagnosis purposes. In this study, a novel portable amperometric graphene oxide–protein biosensor platform is investigated. The main characteristic of this structure is the implementation of a microfluidic configuration. With albumin metalloprotein as the biorecognition element, graphene oxide was synthesized and characterized by transmission electron... 

In this study, anti-leukemic enzyme L-asparaginase (E.C.3.5.1.1) from Escherichia coli ATCC 11303 was modified by the microencapsulation technique onto calcium alginate beads. Using response surface methodology (RSM), a three-level full factorial design, the values of concentration of sodium alginate, concentration of calcium chloride, and enzyme loading were investigated to obtain the highest residual L-asparaginase (L-ASNase) activity % (immobilized enzyme activity/free enzyme activity). The effects of the studied factors on immobilization were evaluated The predicted values by the model were close to the experimental values, indicating suitability of the model. The results presented that... 

This research has investigated the effect of certain geometric parameters on cooling performance of three vortex tubes. The influencing parameters include three length/diameter ratios L/D = 10, 25, 40, three nozzle cases and each case with number n = 2, 4, 6 nozzles, three cold orifice/diameter ratios β = 0.389, 0.5, 0.611 and three inlet pressures Pi = 2, 2.5 and 3 bar. The experiments are conducted based on three factors, two-level and central composite face-centred design with full factorial. The results are analysed according to the principle of response surface methodology. The goodness of fit of the regression model is inspected using the analysis of variance and F-ratio test. The... 

In this study, two moving-bed biofilm reactors (MBBR1 and MBBR2) filled with different size of carrier media (Kaldnes K1 and Kaldnes K1 micro, respectively) were subjected to soluble (sugar and sodium acetate (Ac)) substrate and mixture of soluble and particulate (particulate potato starch (PS)) substrate in a very high organic loading rate (12 kgCOD/m3·d) at different temperatures (26 and 15°C, in MBBR1 and MBBR2, respectively). The effects of carrier type and substrate on biofilm structure and reactor performance have been studied. Starch was removed by adsorption at the biofilm surface and hydrolyzed which caused substrate gradient in MBBR1, however, hydrolyzed uniformly within biofilm in... 

This work has focused on in-situ crosslinking of gelatin (G) to produce electrospun scaffold with improved fiber morphology retention and mechanical properties. As per this approach, we prepared G nanofibers through mixing G, 1-ethyl-3-(3 dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) in the new solvent system. Response surface methodology (RSM) was employed to study the influence of solution parameters on fiber diameter. The morphological structure was examined, and the appropriate level of setting to obtain smooth fibers with a favorable diameter was reported. Results revealed using EDC/NHS for in-situ crosslinking improves the mechanical properties... 

In this study, modeling of an irreversible thermoelectric heat pump was conducted, and its performance was assessed in terms of exergy for 10, 20, 30 and 40 K difference in temperature (∆T) by changing the values of the design parameters. By employing this model, positive impact of increasing cross-section area, current and thermocouple’s length which in turn increases the exergy efficiency is realized. In addition, diminishing adverse impact of adding more thermocouples on the exergy efficiency of the system is illustrated. Afterward, exergoeconomic performance of the thermoelectric heat pump is evaluated. Then, exergoeconomic factor for each of the system’s components is diagnosed. The... 

This work has focused on in-situ crosslinking of gelatin (G) to produce electrospun scaffold with improved fiber morphology retention and mechanical properties. As per this approach, we prepared G nanofibers through mixing G, 1-ethyl-3-(3 dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) in the new solvent system. Response surface methodology (RSM) was employed to study the influence of solution parameters on fiber diameter. The morphological structure was examined, and the appropriate level of setting to obtain smooth fibers with a favorable diameter was reported. Results revealed using EDC/NHS for in-situ crosslinking improves the mechanical properties... 

Investigations have shown the traces of antibiotics in surface water, groundwater, wastewater treatment plants, and drinking water. However, conventional wastewater treatment is not entirely effective for vancomycin degradation. Advanced oxidation is one of the most widespread methods of antibiotic degradation in aqueous media. Vancomycin was quantified by high-performance liquid chromatography. The Response Surface Methodology (RSM) based on Central Composite Design (CCD) was used to explore and optimise the effect of the independent variables on vancomycin degradation. Independent variables were as follows: pH (3–11), vancomycin concentration (15–75 mg/L), TiO2–clinoptilolite (25–125 mg in... 

This research has investigated the effect of certain geometric parameters on cooling performance of three vortex tubes. The influencing parameters include three length/diameter ratios L/D = 10, 25, 40, three nozzle cases and each case with number n = 2, 4, 6 nozzles, three cold orifice/diameter ratios β = 0.389, 0.5, 0.611 and three inlet pressures Pi = 2, 2.5 and 3 bar. The experiments are conducted based on three factors, two-level and central composite face-centred design with full factorial. The results are analysed according to the principle of response surface methodology. The goodness of fit of the regression model is inspected using the analysis of variance and F-ratio test. The... 

Comparative studies between response surface methodology (RSM) and artificial neural network (ANN) methods to find the effects of electrospinning parameters on the porosity of nanofiber mats is described. The four important electrospinning parameters studied included solution concentration (wt.%), applied voltage (kV), spinning distance (cm) and volume flow rate (mL/h). It was found that the applied voltage and solution concentration are the two critical parameters affecting the porosity of the nanofiber mats. The two approaches were compared for their modeling and optimization capabilities with the modeling capability of RSM showing superiority over ANN, having comparatively lower values of... 

Availability of hydrocarbon gases at the field makes it attractive for gas-based enhanced oil recovery (EOR) methods such as water alternating gas (WAG) injection. Optimization of EOR methods requires too many simulation runs, which are time consuming and expensive. Therefore, developing a proxy model, which emulates simulator outputs, is considered as an appropriate alternative technique. In this work, effects of composition changes of injection gas, WAG ratio, and slug size on produced oil and water were investigated. In addition, the optimum value of the previous parameters, including some constraints, are presented using response surface methodology. Experimental design is also applied... 

As the ordinary least squares (OLS) method is very sensitive to outliers as well as to correlated responses, a robust coefficient estimation method is proposed in this paper for multi-response surfaces in multistage processes based on M-estimators. In this approach, experimental designs are used in which the intermediate response variables may act as covariates in the next stages. The performances of both the ordinary multivariate OLS and the proposed robust multi-response surface approach are analyzed and compared through extensive simulation experiments. Sum of the squared errors in estimating the regression coefficients reveals the efficiency of the proposed robust approach. © 2017 Taylor... 

In this study, a three-factor, three-level Box-Behnken design with response surface methodology were used to maximize the TOC removal and minimize the H2O2 residual in the effluent of the combined UV-C/H2O2-VUV system for the treatment of an actual slaughterhouse wastewater (SWW) collected from one of the meat processing plants in Ontario, Canada. The irradiation time and the initial concentrations of total organic carbon (TOCo) and hydrogen peroxide (H2O2o) were the three predictors, as independent variables, studied in the design of experiments. The multiple response approach was used to obtain desirability response surfaces at the optimum factor settings. Subsequently, the optimum... 

In this study, asphaltene deposition from crude oil has been studied experimentally using a test loop and prediction using theoretical study under turbulent flow (Reynolds numbers below 5000). The effects of many parameters such as oil velocity, surface temperature and concentration of flocculated asphaltene on the asphaltene deposition were investigated. The results showed that asphaltene deposition thickness increases with increasing both surface temperature and concentration of flocculated asphaltene and decreasing oil velocity. Thermal approach was used to describe the mechanisms involved in this process and the results of data fitting showed that there are good agreements between the... 

As the ordinary least squares (OLS) method is very sensitive to outliers as well as to correlated responses, a robust coefficient estimation method is proposed in this paper for multi-response surfaces in multistage processes based on M-estimators. In this approach, experimental designs are used in which the intermediate response variables may act as covariates in the next stages. The performances of both the ordinary multivariate OLS and the proposed robust multi-response surface approach are analyzed and compared through extensive simulation experiments. Sum of the squared errors in estimating the regression coefficients reveals the efficiency of the proposed robust approach. © 2018 Taylor... 

Background: Integrated sewage sludge handling and heavy metal management are important issues that scientists are working to solve today. Methods: Hydrothermal carbonization of dewatered digested sewage sludge (DDSS) under various conditions is carried out in this work, followed by alkali activation. The response surface methodology is used to investigate the operating process conditions and optimize them in order to produce hydrochar with the highest modified adsorption capacity (yield and Pb2+ adsorption). Significant Findings: The ideal conditions are 182.4°C, 4.9 hours, 5.025 (w/w) water/DDSS ratio, and 3.5 (w/w) ZnCl2/DDSS ratio. In addition, the Langmuir isotherm (qmax =109.3 mg/g) and...