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    Purification and characterization of a novel biosurfactant produced by Bacillus licheniformis MS3

    , Article World Journal of Microbiology and Biotechnology ; Volume 26, Issue 5 , 2010 , Pages 871-878 ; 09593993 (ISSN) Biria, D ; Maghsoudi, E ; Roostaazad, R ; Dadafarin, H ; Sahebghadam Lotfi, A ; Amoozegar, M. A ; Sharif University of Technology
    2010
    Abstract
    The physical properties and chemical structure of a new biosurfactant (licheniformin) produced by Bacillus licheniformis MS3 were investigated. The purified biosurfactant was identified as a lipopeptide with amino acid sequence of Gly, Ala, Val, Asp, Ser, Gly, Tyr and a lactone linkage between the carboxyl group of Aspargine and hydroxyl group of Tyrosine residue. The fatty acid moiety was attached to N-terminal amino acid residue through an amide bond. The purified licheniformin could lower the surface tension of water from 72 to 38 mN/m at concentrations higher than 15 μg/mL and its relative emulsion volume (EV%) was equal to 36%. It also showed stable surface activity over a wide range of...