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    Fluidic barriers in droplet-based centrifugal microfluidics: Generation of multiple emulsions and microspheres

    , Article Sensors and Actuators, B: Chemical ; Volume 311 , May , 2020 Madadelahi, M ; Madou, M. J ; Dorri Nokoorani, Y ; Shamloo, A ; Martinez Chapa, S. O ; Sharif University of Technology
    Elsevier B. V  2020
    Droplet generation is very important in biochemical processes such as cell encapsulation, digital PCR (Polymerase Chain Reaction), and drug delivery. In the present paper, a density-based method called “fluidic barrier” is introduced to produce multiple emulsions on a centrifugal microfluidic platform or Lab-on-a-CD (LOCD). We show that the density and the viscosity of the fluids involved are important parameters for predicting the characteristics of the droplets generated with this method. Moreover, we broadened this concept by using the fluidic barriers to separate reactive chemicals. As a proof of concept, alginate and CaCl2 solutions are separated by an oil barrier to control the... 

    Enzymatic outside-in cross-linking enables single-step microcapsule production for high-throughput three-dimensional cell microaggregate formation

    , Article Materials Today Bio ; Volume 6 , 2020 Van Loo, B ; Salehi, S. S ; Henke, S ; Shamloo, A ; Kamperman, T ; Karperien, M ; Leijten, J ; Sharif University of Technology
    Elsevier B.V  2020
    Cell-laden hydrogel microcapsules enable the high-throughput production of cell aggregates, which are relevant for three-dimensional tissue engineering and drug screening applications. However, current microcapsule production strategies are limited by their throughput, multistep protocols, and limited amount of compatible biomaterials. We here present a single-step process for the controlled microfluidic production of single-core microcapsules using enzymatic outside-in cross-linking of tyramine-conjugated polymers. It was hypothesized that a physically, instead of the conventionally explored biochemically, controlled enzymatic cross-linking process would improve the reproducibility,... 

    Volume and concentration dosing in picolitres using a two-channel microfluidic AFM cantilever

    , Article Nanoscale ; Volume 12, Issue 18 , 2020 , Pages 10292-10305 Verlinden, E. J ; Madadelahi, M ; Sarajlic, E ; Shamloo, A ; Engel, A. H ; Staufer, U ; Ghatkesar, M. K ; Sharif University of Technology
    NLM (Medline)  2020
    We introduce a two-channel microfluidic atomic force microscopy (AFM) cantilever that combines the nanomechanical sensing functionality of an AFM cantilever with the ability to manipulate fluids of picolitres or smaller volumes through nanoscale apertures near the cantilever tip. Each channel is connected to a separate fluid reservoir, which can be independently controlled by pressure. Various systematic experiments with fluorescent liquids were done by either injecting the liquids from the on-chip reservoir or aspirating directly through the nanoscale apertures at the tip. A flow rate analysis of volume dosing, aspiration and concentration dosing inside the liquid medium was performed. To... 

    Three-dimensional bioprinting of functional skeletal muscle tissue using gelatin methacryloyl-alginate bioinks

    , Article Micromachines ; Volume 10, Issue 10 , 2019 ; 2072666X (ISSN) Seyedmahmoud, R ; Çelebi Saltik, B ; Barros, N ; Nasiri, R ; Banton, E ; Shamloo, A ; Ashammakhi, N ; Dokmeci, M. R ; Ahadian, S ; Sharif University of Technology
    MDPI AG  2019
    Skeletal muscle tissue engineering aims to fabricate tissue constructs to replace or restore diseased or injured skeletal muscle tissues in the body. Several biomaterials and microscale technologies have been used in muscle tissue engineering. However, it is still challenging to mimic the function and structure of the native muscle tissues. Three-dimensional (3D) bioprinting is a powerful tool to mimic the hierarchical structure of native tissues. Here, 3D bioprinting was used to fabricate tissue constructs using gelatin methacryloyl (GelMA)-alginate bioinks. Mechanical and rheological properties of GelMA-alginate hydrogels were characterized. C2C12 myoblasts at the density 8 × 106 cells/mL... 

    Gut-on-a-chip: Current progress and future opportunities

    , Article Biomaterials ; Volume 255 , 2020 Ashammakhi, N ; Nasiri, R ; Barros, N. R. D ; Tebon, P ; Thakor, J ; Goudie, M ; Shamloo, A ; Martin, M. G ; Khademhosseni, A ; Sharif University of Technology
    Elsevier Ltd  2020
    Organ-on-a-chip technology tries to mimic the complexity of native tissues in vitro. Important progress has recently been made in using this technology to study the gut with and without microbiota. These in vitro models can serve as an alternative to animal models for studying physiology, pathology, and pharmacology. While these models have greater physiological relevance than two-dimensional (2D) cell systems in vitro, endocrine and immunological functions in gut-on-a-chip models are still poorly represented. Furthermore, the construction of complex models, in which different cell types and structures interact, remains a challenge. Generally, gut-on-a-chip models have the potential to... 

    3D Bioprinting of oxygenated cell-laden gelatin methacryloyl constructs

    , Article Advanced Healthcare Materials ; Volume 9, Issue 15 , 2020 Erdem, A ; Darabi, M. A ; Nasiri, R ; Sangabathuni, S ; Ertas, Y. N ; Alem, H ; Hosseini, V ; Shamloo, A ; Nasr, A. S ; Ahadian, S ; Dokmeci, M. R ; Khademhosseini, A ; Ashammakhi, N ; Sharif University of Technology
    Wiley-VCH Verlag  2020
    Cell survival during the early stages of transplantation and before new blood vessels formation is a major challenge in translational applications of 3D bioprinted tissues. Supplementing oxygen (O2) to transplanted cells via an O2 generating source such as calcium peroxide (CPO) is an attractive approach to ensure cell viability. Calcium peroxide also produces calcium hydroxide that reduces the viscosity of bioinks, which is a limiting factor for bioprinting. Therefore, adapting this solution into 3D bioprinting is of significant importance. In this study, a gelatin methacryloyl (GelMA) bioink that is optimized in terms of pH and viscosity is developed. The improved rheological properties... 

    Microfluidic-based approaches in targeted cell/particle separation based on physical properties: fundamentals and applications

    , Article Small ; Volume 16, Issue 29 , 2020 Nasiri, R ; Shamloo, A ; Ahadian, S ; Amirifar, L ; Akbari, J ; Goudie, M. J ; Lee, K ; Ashammakhi, N ; Dokmeci, M. R ; Di Carlo, D ; Khademhosseini, A ; Sharif University of Technology
    Wiley-VCH Verlag  2020
    Cell separation is a key step in many biomedical research areas including biotechnology, cancer research, regenerative medicine, and drug discovery. While conventional cell sorting approaches have led to high-efficiency sorting by exploiting the cell's specific properties, microfluidics has shown great promise in cell separation by exploiting different physical principles and using different properties of the cells. In particular, label-free cell separation techniques are highly recommended to minimize cell damage and avoid costly and labor-intensive steps of labeling molecular signatures of cells. In general, microfluidic-based cell sorting approaches can separate cells using “intrinsic”... 

    Bacterial receiver prototype for molecular communication using rhamnose operon in a microfluidic environment

    , Article IEEE Transactions on Nanobioscience ; Volume 20, Issue 4 , 2021 , Pages 426-435 ; 15361241 (ISSN) Amerizadeh, A ; Mashhadian, A ; Farahnak Ghazani, M ; Arjmandi, H. R ; Alsadat Rad, M ; Shamloo, A ; Vosoughi, M ; Nasiri Kenari, M ; Sharif University of Technology
    Institute of Electrical and Electronics Engineers Inc  2021
    Bacterial populations are promising candidates for the development of the receiver and transmitter nanomachines for molecular communication (MC). A bacterial receiver is required to uptake the information molecules and produce the detectable molecules following a regulation mechanism. We have constructed a novel bacterial MC receiver using an inducible bacterial L-rhamnose-regulating operon. The proposed bacterial receiver produces green fluorescent protein (GFP) in response to the L-rhamnose information molecules following a quite fast regulation mechanism. To fabricate the receiver, the bacterial population has been transformed using a plasmid harboring L-rhamnose operon genes and gene... 

    Healthy and diseasedin vitromodels of vascular systems

    , Article Lab on a Chip ; Volume 21, Issue 4 , 2021 , Pages 641-659 ; 14730197 (ISSN) Hosseini, V ; Mallone, A ; Nasrollahi, F ; Ostrovidov, S ; Nasiri, R ; Mahmoodi, M ; Haghniaz, R ; Baidya, A ; Salek, M. M ; Darabi, M. A ; Orive, G ; Shamloo, A ; Dokmeci, M. R ; Ahadian, S ; Khademhosseini, A ; Sharif University of Technology
    Royal Society of Chemistry  2021
    Irregular hemodynamics affects the progression of various vascular diseases, such atherosclerosis or aneurysms. Despite the extensive hemodynamics studies on animal models, the inter-species differences between humans and animals hamper the translation of such findings. Recent advances in vascular tissue engineering and the suitability ofin vitromodels for interim analysis have increased the use ofin vitrohuman vascular tissue models. Although the effect of flow on endothelial cell (EC) pathophysiology and EC-flow interactions have been vastly studied in two-dimensional systems, they cannot be used to understand the effect of other micro- and macro-environmental parameters associated with... 

    Combined effects of electric stimulation and microgrooves in cardiac tissue-on-a-chip for drug screening

    , Article Small Methods ; Volume 4, Issue 10 , 2020 Ren, L ; Zhou, X ; Nasiri, R ; Fang, J ; Jiang, X ; Wang, C ; Qu, M ; Ling, H ; Chen, Y ; Xue, Y ; Hartel, M.C ; Tebon, P ; Zhang, S ; Kim, H.-J ; Yuan, X ; Shamloo, A ; Dokmeci, M. R ; Li, S ; Khademhosseini, A ; Ahadian, S ; Sun, W ; Sharif University of Technology
    John Wiley and Sons Inc  2020
    Animal models and traditional cell cultures are essential tools for drug development. However, these platforms can show striking discrepancies in efficacy and side effects when compared to human trials. These differences can lengthen the drug development process and even lead to drug withdrawal from the market. The establishment of preclinical drug screening platforms that have higher relevancy to physiological conditions is desirable to facilitate drug development. Here, a heart-on-a-chip platform, incorporating microgrooves and electrical pulse stimulations to recapitulate the well-aligned structure and synchronous beating of cardiomyocytes (CMs) for drug screening, is reported. Each chip...