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Mixed oxide nanotubes in nanomedicine: A dead-end or a bridge to the future?
, Article Ceramics International ; 2020 ; Nasiri Tabrizi, B ; Yeong, C. H ; Madaah Hosseini, H. R ; Saber Samandari, S ; Basirun, W. J ; Tsuzuki, T ; Sharif University of Technology
Elsevier Ltd
2020
Abstract
Nanomedicine has seen a significant rise in the development of new research tools and clinically functional devices. In this regard, significant advances and new commercial applications are expected in the pharmaceutical and orthopedic industries. For advanced orthopedic implant technologies, appropriate nanoscale surface modifications are highly effective strategies and are widely studied in the literature for improving implant performance. It is well-established that implants with nanotubular surfaces show a drastic improvement in new bone creation and gene expression compared to implants without nanotopography. Nevertheless, the scientific and clinical understanding of mixed oxide...
Temporal activation of LRH-1 and RAR-γ in human pluripotent stem cells induces a functional naïve-like state
, Article EMBO Reports ; Volume 21, Issue 10 , 2020 ; Kiani, T ; Taghizadeh, Z ; Moradi, S ; Samadian, A ; Mollamohammadi, S ; Sharifi Zarchi, A ; Guenther, S ; Akhlaghpour, A ; Asgari Abibeiglou, B ; Najar Asl, M ; Karamzadeh, R ; Khalooghi, K ; Braun, T ; Hassani, S. N ; Baharvand, H ; Sharif University of Technology
Wiley-VCH Verlag
2020
Abstract
Naïve pluripotency can be established in human pluripotent stem cells (hPSCs) by manipulation of transcription factors, signaling pathways, or a combination thereof. However, differences exist in the molecular and functional properties of naïve hPSCs generated by different protocols, which include varying similarities with pre-implantation human embryos, differentiation potential, and maintenance of genomic integrity. We show here that short treatment with two chemical agonists (2a) of nuclear receptors, liver receptor homologue-1 (LRH-1) and retinoic acid receptor gamma (RAR-γ), along with 2i/LIF (2a2iL) induces naïve-like pluripotency in human cells during reprogramming of fibroblasts,...
Core-sheath gelatin based electrospun nanofibers for dual delivery release of biomolecules and therapeutics
, Article Materials Science and Engineering C ; Volume 108 , 2020 ; Lotfi, R ; Tamjid, E ; Shokrgozar, M. A ; Simchi, A ; Sharif University of Technology
Elsevier Ltd
2020
Abstract
Coaxial electrospinning with the ability to use simultaneously two separate solvents provides a promising strategy for drug delivery. Nevertheless, controlled release of hydrophilic and sensitive therapeutics from slow biodegradable polymers is still challenging. To address this gap, we fabricated core-sheath fibers for dual delivery of lysozyme, as a model protein, and phenytoin sodium as a small therapeutic molecule. The sheath was processed by a gelatin solution while the core fibers were fabricated from an aqueous gelatin/PVA solution. Microstructural studies by transmission and scanning electron microscopy reveal the formation of homogeneous core-sheath nanofibers with an outer and...
The controlled release of dexamethasone sodium phosphate from bioactive electrospun PCL/gelatin nanofiber scaffold
, Article Iranian Journal of Pharmaceutical Research ; Volume 18, Issue 1 , 2019 , Pages 111-124 ; 17350328 (ISSN) ; Mashayekhan, S ; Abbaszadeh, H. A ; Sharif University of Technology
Iranian Journal of Pharmaceutical Research
2019
Abstract
In this study, a system of dexamethasone sodium phosphate (DEXP)-loaded chitosan nanoparticles embedded in poly-ε-caprolacton (PCL) and gelatin electrospun nanofiber scaffold was introduced with potential therapeutic application for treatment of the nervous system. Besides anti-inflammatory properties, DEXP act through its glucocorticoid receptors, which are involved in the inhibition of astrocyte proliferation and microglial activation. Bovine serum albumin (BSA) was used to improve the encapsulation efficiency of DEXP within chitosan nanoparticles and to overcome its initial burst release. BSA incorporation within the chitosan nanoparticles increased the encapsulation efficiency of DEXP...
Design and fabrication of a novel microfluidic system for enrichment of circulating tumor cells with the assistance of computer simulations
, Article Avicenna Journal of Medical Biotechnology ; Volume 11, Issue 4 , 2019 , Pages 277-284 ; 20082835 (ISSN) ; Vossoughi, M ; Alemzadeh, I ; Sharif University of Technology
Avicenna Research Institute
2019
Abstract
Background: Cancer is the first cause of death in developed countries. The heterogeneous nature of cancer requires patient-specified treatment plans. One reliable approach is collecting Circulating Tumour Cells (CTCs) and using them for prognosis and drug response assessment purposes. CTCs are rare and their separation from normal cell requires high-accuracy methods. Methods: A microfluidic cell capture device to separate CTCs from peripheral blood is presented in this study. The CTC separation device applies hydrodynamic forces to categorize cells according to their sizes. The proposed device is designed and evaluated by numerical simulations and validated experimentally. The simulation...
Modification of bacterial cellulose/keratin nanofibrous mats by a tragacanth gum-conjugated hydrogel for wound healing
, Article International Journal of Biological Macromolecules ; Volume 134 , 2019 , Pages 280-289 ; 01418130 (ISSN) ; Tamjid, E ; Eslahi, N ; Simchi, A ; Sharif University of Technology
Elsevier B.V
2019
Abstract
To enhance physicomechanical properties and bioactivity of fibrous membranes for wound dressing and tissue engineering applications, novel composite scaffolds consisting of fibrous mats and thermosensitive hydrogel particles were prepared by concurrent electrospinning and electrospraying technique. The composite scaffolds were composed of keratin/bacterial cellulose fibers (150 ± 43 nm) which are hybridized with hydrogel particles (500 nm to 2 μm) based on nonionic triblock copolymers conjugated with Tragacanth gum (TG). FTIR and H-NMR studies indicated ester reactions between carboxylated copolymers and TG through carbodiimide crosslinker chemistry. The hydrogel particles were uniformly...
A comparative study of wound dressings loaded with silver sulfadiazine and silver nanoparticles: In vitro and in vivo evaluation
, Article International Journal of Pharmaceutics ; Volume 564 , 2019 , Pages 350-358 ; 03785173 (ISSN) ; Shamloo, A ; Aghababaie, Z ; Afjoul, H ; Abdi, S ; Moravvej, H ; Vossoughi, M ; Sharif University of Technology
Elsevier B.V
2019
Abstract
In the current study, two series of antimicrobial dressings conjugated with silver sulfadiazine (SSD) and silver nanoparticles (AgNPs) were developed and evaluated for chronic wound healing. Highly porous polycaprolactone (PCL)/polyvinyl alcohol (PVA) nanofibers were loaded with different concentrations of SSD or AgNPs and compared comprehensively in vitro and in vivo. SSD and AgNPs indicated a strong and equal antimicrobial activity against S. aureus. However, SSD had more toxicity against fibroblast cells over one week in vitro culture. An in vivo model of wound healing on male Wistar rats was developed with a full thickness wound. All the wound dressings indicated enough flexibility and...
Fabrication, modeling and optimization of lyophilized advanced platelet rich fibrin in combination with collagen-chitosan as a guided bone regeneration membrane
, Article International Journal of Biological Macromolecules ; Volume 125 , 2019 , Pages 383-391 ; 01418130 (ISSN) ; Mashayekhan, S ; Saadatmand, M ; Sharif University of Technology
Elsevier B.V
2019
Abstract
In this study, lyophilized advanced platelet rich fibrin (A-PRF) was used in combination with collagen-chitosan membrane for the first time to combine advantages of both collagen and A-PRF membranes. Response surface methodology (RSM) was used to design the experimental condition and to correlate the effects of parameters, including chitosan/collagen (chit/col) weight ratio and A-PRF concentration on Young's modulus, mesenchymal stem cell (MSCs) viability and degradation rate of the membranes. Results showed that Young's modulus of the membranes was intensified by increasing chit/col weight ratio and decreasing A-PRF concentration from 3 to 8 MPa. Cell viability of MSCs was improved by both...
Seasonal variations in the oxidative stress and inflammatory potential of PM2.5 in Tehran using an alveolar macrophage model; The role of chemical composition and sources
, Article Environment International ; Volume 123 , 2019 , Pages 417-427 ; 01604120 (ISSN) ; Antkiewicz, D. S ; Hemming, J. D. C ; Shafer, M. M ; Lai, A. M ; Arhami, M ; Hosseini, V ; Schauer, J. J ; Sharif University of Technology
Elsevier Ltd
2019
Abstract
The current study was designed to assess the association between temporal variations in urban PM2.5 chemical composition, sources, and the oxidative stress and inflammatory response in an alveolar macrophage (AM) model. A year-long sampling campaign collected PM2.5 samples at the Sharif University in Tehran, Iran. PM-induced reactive oxygen species (ROS) production was measured both with an acellular dithiothreitol consumption assay (DTT-ROS; ranged from 2.1 to 9.3 nmoles min−1 m−3) and an in vitro macrophage-mediated ROS production assay (AM-ROS; ranged from 125 to 1213 μg Zymosan equivalents m−3). The production of tumor necrosis factor alpha (TNF-α; ranged from ~60 to 518 pg TNF-α m−3)...
Engineering folate-targeting diselenide-containing triblock copolymer as a redox-responsive shell-sheddable micelle for antitumor therapy in vivo
, Article Acta Biomaterialia ; Volume 76 , 2018 , Pages 239-256 ; 17427061 (ISSN) ; Abdkhodaie, M. J ; Sadeghi Abandansari, H ; Satarian, L ; Molazem, M ; Al Jamal, K. T ; Baharvand, H ; Sharif University of Technology
Acta Materialia Inc
2018
Abstract
The oxidation-reduction (redox)–responsive micelle system is based on a diselenide-containing triblock copolymer, poly(ε-caprolactone)-bis(diselenide-methoxy poly(ethylene glycol)/poly(ethylene glycol)-folate) [PCL-(SeSe-mPEG/PEG-FA)2]. This has helped in the development of tumor-targeted delivery for hydrophobic anticancer drugs. The diselenide bond, as a redox-sensitive linkage, was designed in such a manner that it is located at the hydrophilic–hydrophobic hinge to allow complete collapse of the micelle and thus efficient drug release in redox environments. The amphiphilic block copolymers self-assembled into micelles at concentrations higher than the critical micelle concentration (CMC)...
A microfabricated platform for the study of chondrogenesis under different compressive loads
, Article Journal of the Mechanical Behavior of Biomedical Materials ; Volume 78 , 2018 , Pages 404-413 ; 17516161 (ISSN) ; Jahanbakhsh, A ; Saidi, M. S ; Bonakdar, S ; Sharif University of Technology
Elsevier Ltd
2018
Abstract
Microfluidic devices are beneficial in miniaturizing and multiplexing various cellular assays in a single platform. Chondrogenesis is known to pertain to chemical, topographical, and mechanical cues in the microenvironment. Mechanical cues themselves have numerous parameters such as strain magnitude, frequency, and stimulation time. Effects of different strain magnitudes on the chondrogenic differentiation of adult stem cells have not been explored thoroughly. Here, a new multilayer microdevice is presented for the unidirectional compressive stimulation of cells in a three-dimensional cell culture. Numerical simulations were performed to evaluate and optimize the design. Results showed a...
Producing functional recombinant human keratinocyte growth factor in Pichia pastoris and investigating its protective role against irradiation
, Article Enzyme and Microbial Technology ; Volume 111 , April , 2018 , Pages 12-20 ; 01410229 (ISSN) ; Kalhor, H. R ; Mowla, S. J ; Sharif University of Technology
Elsevier Inc
2018
Abstract
Keratinocyte Growth Factor (KGF) is a paracrine-acting, epithelial mitogen that plays a prominent role in the regeneration of damaged epithelial tissues. In spite of different attempts to produce recombinant human KGF in many organisms, including bacteria, mammalian cells, plant cells and insect cells; production of recombinant form suffers from lower yields and recovery relative to other recombinant proteins of similar size and properties. Due to many advantages of Pichia pastoris expression systems for producing industrial enzymes and pharmaceutical proteins, in this study P. pastoris was chosen as a host for KGF expression. For preparing human KGF coding sequence, MCF-7 cell line was...
The different fate of satellite cells on conductive composite electrospun nanofibers with graphene and graphene oxide nanosheets
, Article Biomedical Materials (Bristol) ; Volume 11, Issue 2 , 2016 ; 17486041 (ISSN) ; Soleimani, M ; Hatamie, S ; Zamanlui, S ; Ranjbarvan, P ; Vossoughi, M ; Hosseinzadeh, S ; Sharif University of Technology
Institute of Physics Publishing
2016
Abstract
Electrospinning of composite polymer solutions provides fantastic potential to prepare novel nanofibers for use in a variety of applications. The addition of graphene (G) and graphene oxide (GO) nanosheets to bioactive polymers was found to enhance their conductivity and biocompatibility. Composite conductive nanofibers of polyaniline (PANI) and polyacrylonitrile (PAN) with G and GO nanosheets were prepared by an electrospinning process. The fabricated membranes were investigated by physical and chemical examinations including scanning electron microscopy (SEM), Raman spectroscopy, x-ray diffraction (XRD) and tensile assay. The muscle satellite cells enriched by a pre-plating technique were...
Directional migration and differentiation of neural stem cells within three-dimensional microenvironments
, Article Integrative Biology (United Kingdom) ; Volume 7, Issue 3 , Jan , 2015 , Pages 335-344 ; 17579694 (ISSN) ; Heibatollahi, M ; Mofrad, M. R. K ; Sharif University of Technology
Royal Society of Chemistry
2015
Abstract
Harnessing neural stem cells to repair neuronal damage is a promising potential treatment for neuronal diseases. To enable future therapeutic efficacy, the survival, proliferation, migration and differentiation of neural stem/progenitor cells (NPCs) should be accurately studied and optimized in in vitro platforms before transplanting these cells into the body for treatment purposes. Such studies can determine the appropriate quantities of the biochemical and biomechanical factors needed to control and optimize NPC behavior in vivo. In this study, NPCs were cultured within a microfluidic device while being encapsulated within the collagen matrix. The migration and differentiation of NPCs were...
Tissue growth into three-dimensional composite scaffolds with controlled micro-features and nanotopographical surfaces
, Article Journal of Biomedical Materials Research - Part A ; Volume 101, Issue 10 , 2013 , Pages 2796-2807 ; 15493296 (ISSN) ; Simchi, A ; Dunlop, J. W. C ; Fratzl, P ; Bagheri, R ; Vossoughi, M ; Sharif University of Technology
2013
Abstract
Controlling topographic features at all length scales is of great importance for the interaction of cells with tissue regenerative materials. We utilized an indirect three-dimensional printing method to fabricate polymeric scaffolds with pre-defined and controlled external and internal architecture that had an interconnected structure with macro- (400-500 μm) and micro- (∼25 μm) porosity. Polycaprolactone (PCL) was used as model system to study the kinetics of tissue growth within porous scaffolds. The surface of the scaffolds was decorated with TiO2 and bioactive glass (BG) nanoparticles to the better match to nanoarchitecture of extracellular matrix (ECM). Micrometric BG particles were...
Optimal robust control of drug delivery in cancer chemotherapy: A comparison between three control approaches
, Article Computer Methods and Programs in Biomedicine ; Volume 112, Issue 1 , 2013 , Pages 69-83 ; 01692607 (ISSN) ; Vossoughi, G ; Salarieh, H ; Sharif University of Technology
2013
Abstract
During the drug delivery process in chemotherapy, both of the cancer cells and normal healthy cells may be killed. In this paper, three mathematical cell-kill models including log-kill hypothesis, Norton-Simon hypothesis and Emax hypothesis are considered. Three control approaches including optimal linear regulation, nonlinear optimal control based on variation of extremals and H∞-robust control based on μ-synthesis are developed. An appropriate cost function is defined such that the amount of required drug is minimized while the tumor volume is reduced. For the first time, performance of the system is investigated and compared for three control strategies; applied on three nonlinear models...
Preparation and evaluation of bioactive and compatible starch based superabsorbent for oral drug delivery systems
, Article Journal of Drug Delivery Science and Technology ; Volume 23, Issue 5 , 2013 , Pages 511-517 ; 17732247 (ISSN) ; Ebrahimi, A. A ; Barzegar, S ; Sharif University of Technology
2013
Abstract
Novel types of highly swelling hydrogels (superabsorbent) were prepared by grafting crosslinked poly acrylic acid-co-2-hydroxyethylmetacrylate (PAA-co-HEMA) chains onto starch through a free radical polymerization method. The effect of grafting variables (i.e., concentration of methylenebisacrylamide (MBA), acrylic acid/2-hydroxy methymetacrylate (AA/HEMA) weight ratio, ammonium persulfate (APS), starch, neutralization percent, were systematically optimized to achieve a hydrogel with a maximum swelling capacity. The superabsorbent (SAP) formation was confirmed by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). The controlled-release behavior of...
Long-term investigation on the phase stability, magnetic behavior, toxicity, and MRI characteristics of superparamagnetic Fe/Fe-oxide core/shell nanoparticles
, Article International Journal of Pharmaceutics ; Volume 439, Issue 1-2 , 2012 , Pages 28-40 ; 03785173 (ISSN) ; Madaah Hosseini, H. R ; Seyed Reyhani, S. M ; Shokrgozar, M. A ; Oghabian, M. A ; Ahmadi, R ; Sharif University of Technology
2012
Abstract
To efficiently enhance the contrast obtaining from magnetic resonance imaging (MRI), pharmaceutical grade colloidal dispersions of PEG coated iron-based nanoparticles were prepared and compared to conventional pure iron oxide contrast agent. In this study, we synthesized ∼14 nm iron nanoparticles via NaBH4 reduction of iron(III) chloride in an aqueous medium. The resulting nanoparticles were further oxidized by two different methods via (CH3)3NO oxygen transferring agent and exposure to oxygen flow. XRD and electron microscopy analyses confirmed the formation of a second layer on the surface of α-Fe core. As magnetic measurements and Mössbauer spectra of 4-months post prepared nanoparticles...
Supramolecular polycaprolactone nanocomposite based on functionalized hydroxyapatite
, Article Journal of Bioactive and Compatible Polymers ; Volume 27, Issue 5 , January , 2012 , Pages 467-480 ; 08839115 (ISSN) ; Shokrollahi, P ; Atai, M ; Omidian, H ; Bagheri, R ; Sharif University of Technology
SAGE
2012
Abstract
Arms bearing ureido-pyrimidinone functional groups with self-association capability (through quadruple hydrogen bonds) were successfully grafted onto hydroxyapatite nanoparticles. The supramolecularly modified nanoparticles (nHApUPy) exhibited enhanced colloidal stability compared to the original hydroxyapatite nanoparticles and were uniformly dispersed in supramolecular polycaprolactone in PCL(UPy)2/HApUPy nanocomposites at different filler loadings. The combined atomic force microscopy, mechanical, and rheological analyses confirmed a high degree of compatibility of HApUPy nanoparticles with the polymer matrix. The temperature dependence of the supramolecular structure in PCL(UPy)2/HApUPy...
The effect of poly(ethylene glycol) coating on colloidal stability of superparamagnetic iron oxide nanoparticles as potential MRI contrast agent
, Article International Journal of Pharmaceutics ; Volume 433, Issue 1-2 , 2012 , Pages 129-141 ; 03785173 (ISSN) ; Madaah Hosseini, H. R ; Shokrgozar, M. A ; Ahmadi, R ; Oghabian, M. A ; Sharif University of Technology
2012
Abstract
Superparamganetic iron oxide-based contrast agents in magnetic resonance imaging (MRI) have offered new possibility for early detection of lymph nodes and their metastases. According to important role of nanoparticle size in biodistribution, magnetite nanoparticles coated with different polyethylene glycol (PEG) concentrations up to 10/1 PEG/iron oxide weight ratio in an ex situ manner. To predict the PEG-coated nanoparticle behavior in biological media, such as blood stream or tissue, colloidal stability evaluation was performed to estimate the coating endurance in different conditions. Accordingly, optical absorbance measurements were conducted in solutions with different values of pH and...