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Producing functional recombinant human keratinocyte growth factor in Pichia pastoris and investigating its protective role against irradiation
, Article Enzyme and Microbial Technology ; Volume 111 , April , 2018 , Pages 12-20 ; 01410229 (ISSN) ; Kalhor, H. R ; Mowla, S. J ; Sharif University of Technology
Elsevier Inc
2018
Abstract
Keratinocyte Growth Factor (KGF) is a paracrine-acting, epithelial mitogen that plays a prominent role in the regeneration of damaged epithelial tissues. In spite of different attempts to produce recombinant human KGF in many organisms, including bacteria, mammalian cells, plant cells and insect cells; production of recombinant form suffers from lower yields and recovery relative to other recombinant proteins of similar size and properties. Due to many advantages of Pichia pastoris expression systems for producing industrial enzymes and pharmaceutical proteins, in this study P. pastoris was chosen as a host for KGF expression. For preparing human KGF coding sequence, MCF-7 cell line was...
Modification of bacterial cellulose/keratin nanofibrous mats by a tragacanth gum-conjugated hydrogel for wound healing
, Article International Journal of Biological Macromolecules ; Volume 134 , 2019 , Pages 280-289 ; 01418130 (ISSN) ; Tamjid, E ; Eslahi, N ; Simchi, A ; Sharif University of Technology
Elsevier B.V
2019
Abstract
To enhance physicomechanical properties and bioactivity of fibrous membranes for wound dressing and tissue engineering applications, novel composite scaffolds consisting of fibrous mats and thermosensitive hydrogel particles were prepared by concurrent electrospinning and electrospraying technique. The composite scaffolds were composed of keratin/bacterial cellulose fibers (150 ± 43 nm) which are hybridized with hydrogel particles (500 nm to 2 μm) based on nonionic triblock copolymers conjugated with Tragacanth gum (TG). FTIR and H-NMR studies indicated ester reactions between carboxylated copolymers and TG through carbodiimide crosslinker chemistry. The hydrogel particles were uniformly...
A comparative study of wound dressings loaded with silver sulfadiazine and silver nanoparticles: In vitro and in vivo evaluation
, Article International Journal of Pharmaceutics ; Volume 564 , 2019 , Pages 350-358 ; 03785173 (ISSN) ; Shamloo, A ; Aghababaie, Z ; Afjoul, H ; Abdi, S ; Moravvej, H ; Vossoughi, M ; Sharif University of Technology
Elsevier B.V
2019
Abstract
In the current study, two series of antimicrobial dressings conjugated with silver sulfadiazine (SSD) and silver nanoparticles (AgNPs) were developed and evaluated for chronic wound healing. Highly porous polycaprolactone (PCL)/polyvinyl alcohol (PVA) nanofibers were loaded with different concentrations of SSD or AgNPs and compared comprehensively in vitro and in vivo. SSD and AgNPs indicated a strong and equal antimicrobial activity against S. aureus. However, SSD had more toxicity against fibroblast cells over one week in vitro culture. An in vivo model of wound healing on male Wistar rats was developed with a full thickness wound. All the wound dressings indicated enough flexibility and...
Fabrication, modeling and optimization of lyophilized advanced platelet rich fibrin in combination with collagen-chitosan as a guided bone regeneration membrane
, Article International Journal of Biological Macromolecules ; Volume 125 , 2019 , Pages 383-391 ; 01418130 (ISSN) ; Mashayekhan, S ; Saadatmand, M ; Sharif University of Technology
Elsevier B.V
2019
Abstract
In this study, lyophilized advanced platelet rich fibrin (A-PRF) was used in combination with collagen-chitosan membrane for the first time to combine advantages of both collagen and A-PRF membranes. Response surface methodology (RSM) was used to design the experimental condition and to correlate the effects of parameters, including chitosan/collagen (chit/col) weight ratio and A-PRF concentration on Young's modulus, mesenchymal stem cell (MSCs) viability and degradation rate of the membranes. Results showed that Young's modulus of the membranes was intensified by increasing chit/col weight ratio and decreasing A-PRF concentration from 3 to 8 MPa. Cell viability of MSCs was improved by both...
Preparation and characterization of self-electrical stimuli conductive gellan based nano scaffold for nerve regeneration containing chopped short spun nanofibers of PVDF/MCM41 and polyaniline/graphene nanoparticles: Physical, mechanical and morphological studies
, Article International Journal of Biological Macromolecules ; Volume 167 , 2021 , Pages 881-893 ; 01418130 (ISSN) ; S. A., A. R ; H Shirazi, F ; Nemati, N. H ; Sharif University of Technology
Elsevier B.V
2021
Abstract
Conductive self -electrical stimuli bioactive scaffolds could be used the potential for peripheral nerve regeneration with the maximum efficiency. To produce such conductive self-electrical stimuli bioactive scaffolds, chopped spun piezoelectric nanofibers of polyvinylidene fluoride/mesoporous silica nanoparticle (PVDF/MCM41) are prepared and incorporated in gellan/polyaniline/graphene (gellan/PAG) nanocomposites which have been previously prepared by incorporation of polyaniline/graphene (PAG) nanoparticles in gellan gel at 80 °C. Highly conductive binary doped polyaniline/graphene nanoparticles are prepared by chemical oxidative polymerization of aniline monomer using in-suite...
Fabrication and evaluation of chitosan/gelatin/PVA hydrogel incorporating honey for wound healing applications: An in vitro, in vivo study
, Article International Journal of Pharmaceutics ; Volume 592 , 2021 ; 03785173 (ISSN) ; Aghababaie, Z ; Afjoul, H ; Jami, M ; Bidgoli, M. R ; Vossoughi, M ; Ramazani, A ; Kamyabhesari, K ; Sharif University of Technology
Elsevier B.V
2021
Abstract
In this study, physically cross-linked hydrogels were developed by freezing-thawing method while different concentrations of honey were included into the hydrogels for accelerated wound healing. The hydrogel was composed of chitosan, polyvinyl alcohol (PVA), and gelatin with the ratio of 2:1:1 (v/v), respectively. Further, the effect of honey concentrations on antibacterial properties, and cell behavior was investigated. In vivo studies, including wound healing mechanism using rat model and histological analysis of section tissue samples were performed. The results illustrated that the incorporation of honey in hydrogels increased the ultimate strain of hydrogels approximately two times,...
Alginate-based multifunctional films incorporated with sulfur quantum dots for active packaging applications
, Article Colloids and Surfaces B: Biointerfaces ; Volume 215 , 2022 ; 09277765 (ISSN) ; Priyadarshi, R ; Rhim, J. W ; Lotfali, E ; Bagheri, R ; Pircheraghi, G ; Sharif University of Technology
Elsevier B.V
2022
Abstract
Sulfur quantum dots (SQDs) were fabricated using a facile hydrothermal method and used for the preparation of functional food packaging film and compared the properties with other sulfur-based fillers like elemental sulfur (ES) and sulfur nanoparticles (SNP). The SQDs have an average size of 5.3 nm and were very stable in aqueous suspension. Unlike other sulfur-based fillers, the SQD showed high antioxidant, antibacterial and antifungal activity, but no cytotoxicity was found for L929 mouse fibroblasts even after long-term exposure of 48 h. When sulfur-based fillers were added to the alginate film, SQD was more evenly dispersed in the polymer matrix than SNP and ES. The addition of SQD to...
Graphene/cobalt nanocarrier for hyperthermia therapy and MRI diagnosis
, Article Colloids and Surfaces B: Biointerfaces ; Volume 146 , 2016 , Pages 271-279 ; 09277765 (ISSN) ; Ahadian, M. M ; Ghiass, M. A ; Iraji zad, A ; Saber, R ; Parseh, B ; Oghabian, M. A ; Shanehsazzadeh, S ; Sharif University of Technology
Elsevier
Abstract
Graphene/cobalt nanocomposites are promising materials for theranostic nanomedicine applications, which are defined as the ability to diagnose, provide targeted therapy and monitor the response to the therapy. In this study, the composites were synthesized via chemical method, using graphene oxide as the source material and assembling cobalt nanoparticles of 15 nm over the surface of graphene sheets. Various characterization techniques were then employed to reveal the morphology, size and structure of the nanocomposites, such as X-ray diffraction analysis, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, high resolution transmission electron microscopy and...
Hyperthermia of breast cancer tumor using graphene oxide-cobalt ferrite magnetic nanoparticles in mice
, Article Journal of Drug Delivery Science and Technology ; Volume 65 , 2021 ; 17732247 (ISSN) ; Balasi, Z. M ; Ahadian, M. M ; Mortezazadeh, T ; Shams, F ; Hosseinzadeh, S ; Sharif University of Technology
Editions de Sante
2021
Abstract
Herein, the graphene oxide (GO)/cobalt ferrite nanoparticles were used to apply the heat treatment on the breast cancer cell line of MCF7. The synthesized nanoparticles were evaluated before in vitro and in vivo studies, using transmission electron microscopy (TEM), X-ray diffraction (XRD), vibrating sample magnetometer (VSM), X-ray photoelectron spectroscopy (XPS), thermal property and relaxivity measurement. The nanoparticles showed a diameter of 5 nm with the ferrimagnetic property. Also, the nanoparticles were well distributed on the GO nanosheets. The related peaks of cobalt ferrite nanoparticles were approved by using XRD and XPS assays. During the in vitro investigations, IC50 with...
A smart tri-layered nanofibrous hydrogel thin film with controlled release of dual drugs for chemo-thermal therapy of breast cancer
, Article Journal of Drug Delivery Science and Technology ; Volume 76 , 2022 ; 17732247 (ISSN) ; Mohammadi Ziarani, G ; Badiei, A ; Pourjavadi, A ; Kiani, M ; Sharif University of Technology
Editions de Sante
2022
Abstract
A tri-layered nanofibrous hydrogel thin film with temperature sensitivity is introduced as a new controlled drug release system to treat breast cancer. A simultaneous heat generation with the tunable release of dual drugs is observed in response to visible light radiation. A tri-layered nanofibrous sheet was fabricated through sequential electrospinning the blends of Au@Chit@DOX-loaded poly(N-isopropylacrylamide-co-N-methylol acrylamide) (poly (NIPAAm-co-NMA)) and Curcumin-loaded poly (vinyl alcohol) (Cu-loaded PVA), where the Cu-loaded PVA nanofibers (NFs) are sandwiched between two layers of Au@Chit@DOX-loaded poly (NIPAAm-co-NMA) NFs. After thermal crosslinking of the tri-layered...
Bioinspired nanofiber scaffold for differentiating bone marrow-derived neural stem cells to oligodendrocyte-like cells: Design, fabrication, and characterization
, Article International Journal of Nanomedicine ; Volume 15 , 2020 , Pages 3903-3920 ; Mashayekhan, S ; Abbaszadeh, H. A ; Ansarizadeh, M ; Khoramgah, M. S ; Rahimi Movaghar, V ; Sharif University of Technology
Dove Medical Press Ltd
2020
Abstract
Background: Researchers are trying to study the mechanism of neural stem cells (NSCs) differentiation to oligodendrocyte-like cells (OLCs) as well as to enhance the selective differentiation of NSCs to oligodendrocytes. However, the limitation in nerve tissue acces-sibility to isolate the NSCs as well as their differentiation toward oligodendrocytes is still challenging. Purpose: In the present study, a hybrid polycaprolactone (PCL)-gelatin nanofiber scaffold mimicking the native extracellular matrix and axon morphology to direct the differentiation of bone marrow-derived NSCs to OLCs was introduced. Materials and Methods: In order to achieve a sustained release of T3, this factor was...
Graphene oxide negatively regulates cell cycle in embryonic fibroblast cells
, Article International Journal of Nanomedicine ; Volume 15 , 2020 , Pages 6201-6209 ; Akhavan, O ; Shamsara, M ; Ansari Majd, S ; Sanati, M. H ; Daliri Joupari, M ; Farmany, A ; Sharif University of Technology
Dove Medical Press Ltd
2020
Abstract
Background: Unique properties of graphene and its derivatives make them attractive in the field of nanomedicine. However, the mass application of graphene might lead to side effects, which has not been properly addressed in previous studies, especially with regard to its effect on the cell cycle. Methods: The effect of two concentrations (100 and 200 μg/mL) of nano-and microsized graphene oxide (nGO and mGO) on apoptosis, cell cycle, and ROS generation was studied. The effect of both sizes on viability and genotoxicity of the embryonic fibroblast cell cycle was evaluated. MTT and flow cytometry were applied to evaluate the effects of graphene oxide (GO) nanosheets on viability of cells....
Type V collagen in scar tissue regulates the size of scar after heart injury
, Article Cell ; Volume 182, Issue 3 , 2020 , Pages 545-562.e23 ; McCourt, J ; Ma, F ; Ren, S ; Li, S ; Kim, T. H ; Kurmangaliyev, Y. Z ; Nasiri, R ; Ahadian, S ; Nguyen, T ; Tan, X. H. M ; Zhou, Y ; Wu, R ; Rodriguez, A ; Cohn, W ; Wang, Y ; Whitelegge, J ; Ryazantsev, S ; Khademhosseini, A ; Teitell, M. A ; Chiou, P. Y ; Birk, D. E ; Rowat, A. C ; Crosbie, R. H ; Pellegrini, M ; Seldin, M ; Lusis, A. J ; Deb, A ; Sharif University of Technology
Cell Press
2020
Abstract
Scar tissue size following myocardial infarction is an independent predictor of cardiovascular outcomes, yet little is known about factors regulating scar size. We demonstrate that collagen V, a minor constituent of heart scars, regulates the size of heart scars after ischemic injury. Depletion of collagen V led to a paradoxical increase in post-infarction scar size with worsening of heart function. A systems genetics approach across 100 in-bred strains of mice demonstrated that collagen V is a critical driver of postinjury heart function. We show that collagen V deficiency alters the mechanical properties of scar tissue, and altered reciprocal feedback between matrix and cells induces...
Oncolytic newcastle disease virus delivered by mesenchymal stem cells-engineered system enhances the therapeutic effects altering tumor microenvironment
, Article Virology Journal ; Volume 17, Issue 1 , 2020 ; Ebrahimzadeh, M. S ; Miri, S. M ; Dianat Moghadam, H ; Ghorbanhosseini, S. S ; Mohebbi, S. R ; Keyvani, H ; Ghaemi, A ; Sharif University of Technology
BioMed Central Ltd
2020
Abstract
Background: Human papillomavirus (HPV)-associated malignancy remain a main cause of cancer in men and women. Cancer immunotherapy has represented great potential as a new promising cancer therapeutic approach. Here, we report Mesenchymal stem cells (MSCs) as a carrier for the delivery of oncolytic Newcastle disease virus (NDV) for the treatment of HPV-associated tumor. Methods: For this purpose, MSCs obtained from the bone marrow of C57BL mice, then cultured and characterized subsequently by the flow cytometry analysis for the presence of cell surface markers. In this study, we sought out to determine the impacts of MSCs loaded with oncolytic NDV on splenic T cell and cytokine immune...
Synergy between hemagglutinin 2 (HA2) subunit of influenza fusogenic membrane glycoprotein and oncolytic Newcastle disease virus suppressed tumor growth and further enhanced by Immune checkpoint PD-1 blockade
, Article Cancer Cell International ; Volume 20, Issue 1 , August , 2020 ; Ebrahimzadeh, M. S ; Abdolalipour, E ; Yazdi, M ; Hosseini Ravandi, H ; Ghaemi, A ; Sharif University of Technology
BioMed Central Ltd
2020
Abstract
Background: Newcastle disease virus (NDV) has shown noticeable oncolytic properties, especially against cervical cancer. However, in order to improve the spread rate and oncotoxicity of the virus, employment of other therapeutic reagents would be helpful. It has been shown that some viral fusogenic membrane glycoproteins (FMGs) could facilitate viral propagation and increase the infection rate of tumor cells by oncolytic viruses. Additionally, immune checkpoint blockade has widely been investigated for its anti-tumor effects against several types of cancers. Here, we investigated for the first time whether the incorporation of influenza hemagglutinin-2 (HA2) FMG could improve the oncolytic...
Design and fabrication of a novel microfluidic system for enrichment of circulating tumor cells with the assistance of computer simulations
, Article Avicenna Journal of Medical Biotechnology ; Volume 11, Issue 4 , 2019 , Pages 277-284 ; 20082835 (ISSN) ; Vossoughi, M ; Alemzadeh, I ; Sharif University of Technology
Avicenna Research Institute
2019
Abstract
Background: Cancer is the first cause of death in developed countries. The heterogeneous nature of cancer requires patient-specified treatment plans. One reliable approach is collecting Circulating Tumour Cells (CTCs) and using them for prognosis and drug response assessment purposes. CTCs are rare and their separation from normal cell requires high-accuracy methods. Methods: A microfluidic cell capture device to separate CTCs from peripheral blood is presented in this study. The CTC separation device applies hydrodynamic forces to categorize cells according to their sizes. The proposed device is designed and evaluated by numerical simulations and validated experimentally. The simulation...
Engineering folate-targeting diselenide-containing triblock copolymer as a redox-responsive shell-sheddable micelle for antitumor therapy in vivo
, Article Acta Biomaterialia ; Volume 76 , 2018 , Pages 239-256 ; 17427061 (ISSN) ; Abdkhodaie, M. J ; Sadeghi Abandansari, H ; Satarian, L ; Molazem, M ; Al Jamal, K. T ; Baharvand, H ; Sharif University of Technology
Acta Materialia Inc
2018
Abstract
The oxidation-reduction (redox)–responsive micelle system is based on a diselenide-containing triblock copolymer, poly(ε-caprolactone)-bis(diselenide-methoxy poly(ethylene glycol)/poly(ethylene glycol)-folate) [PCL-(SeSe-mPEG/PEG-FA)2]. This has helped in the development of tumor-targeted delivery for hydrophobic anticancer drugs. The diselenide bond, as a redox-sensitive linkage, was designed in such a manner that it is located at the hydrophilic–hydrophobic hinge to allow complete collapse of the micelle and thus efficient drug release in redox environments. The amphiphilic block copolymers self-assembled into micelles at concentrations higher than the critical micelle concentration (CMC)...
Antitumor effect of therapeutic HPV DNA vaccines with chitosan-based nanodelivery systems
, Article Journal of Biomedical Science ; Vol. 21, issue. 1 , July , 2014 ; ISSN: 10217770 ; Ghaemi, A ; Gorji, A ; Kalhor, H. R ; Sajadian, A ; Tabarraei, A ; Moradi, A ; Atyabi, F ; Kelishadi, M ; Sharif University of Technology
Abstract
Cervical cancer is the second-most-common cause of malignancies in women worldwide, and the oncogenic activity of the human papilloma virus types (HPV) E7 protein has a crucial role in anogenital tumors. In this study, we have designed a therapeutic vaccine based on chitosan nanodelivery systems to deliver HPV-16 E7 DNA vaccine, considered as a tumor specific antigen for immunotherapy of HPV-associated cervical cancer. We have developed a Nano-chitosan (NCS) as a carrier system for intramuscular administration using a recombinant DNA vaccine expressing HPV-16 E7 (NCS-DNA E7 vaccine). NCS were characterized in vitro for their gene transfection ability. Results: The transfection of CS-pEGFP...
Chemometrics-assisted effect-directed analysis of crude and refined oil using comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry
, Article Environmental Science and Technology ; Vol. 48, issue. 5 , 2014 , pp. 3074-3083 ; ISSN: 0013936X ; Thomas, K. V ; Parastar, H ; Diez, S ; Tauler, R ; Bayona, J. M ; Sharif University of Technology
Abstract
An effect-directed analysis (EDA) of fresh and artificially weathered (evaporated, photooxidized) samples of North Sea crude oil and residual heavy fuel oil is presented. Aliphatic, aromatic, and polar oil fractions were tested for the presence of aryl hydrocarbon receptor (AhR) agonist and androgen receptor (AR) antagonist, demonstrating for the first time the AR antagonist effects in the aromatic and, to a lesser extent, polar fractions. An extension of the typical EDA strategy to include an N-way partial least-squares (N-PLS) model capable of relating the comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC × GC-TOFMS) data set to the bioassay data...
Tissue growth into three-dimensional composite scaffolds with controlled micro-features and nanotopographical surfaces
, Article Journal of Biomedical Materials Research - Part A ; Volume 101, Issue 10 , 2013 , Pages 2796-2807 ; 15493296 (ISSN) ; Simchi, A ; Dunlop, J. W. C ; Fratzl, P ; Bagheri, R ; Vossoughi, M ; Sharif University of Technology
2013
Abstract
Controlling topographic features at all length scales is of great importance for the interaction of cells with tissue regenerative materials. We utilized an indirect three-dimensional printing method to fabricate polymeric scaffolds with pre-defined and controlled external and internal architecture that had an interconnected structure with macro- (400-500 μm) and micro- (∼25 μm) porosity. Polycaprolactone (PCL) was used as model system to study the kinetics of tissue growth within porous scaffolds. The surface of the scaffolds was decorated with TiO2 and bioactive glass (BG) nanoparticles to the better match to nanoarchitecture of extracellular matrix (ECM). Micrometric BG particles were...