Search for: animal-model
Total 37 records
Accelerated full-thickness wound healing via sustained bFGF delivery based on a PVA/chitosan/gelatin hydrogel incorporating PCL microspheres, Article International Journal of Pharmaceutics ; Volume 537, Issue 1-2 , 2018 , Pages 278-289 ; 03785173 (ISSN) ; Sarmadi, M ; Aghababaie, Z ; Vossoughi, M ; Sharif University of Technology
Elsevier B.V 2018
Herein, a hybrid hydrogel/microsphere system is introduced for accelerated wound healing by sustained release of basic fibroblast growth factor (bFGF). The hydrogel is composed of a mixture of PVA, gelatin and chitosan. The double-emulsion-solvent-evaporation method was utilized to obtain microspheres composed of PCL, as the organic phase, and PVA, as the aqueous phase. Subsequently, various in-vitro and in-vivo assays were performed to characterize the system. BSA was used to optimize the release mechanism, and encapsulation efficiency in microspheres, where a combination of 3% (w/v) PCL and 1% (w/v) PVA was found to be the optimum microsphere sample. Incorporation of microspheres within...
Nanomedicine and advanced technologies for burns: Preventing infection and facilitating wound healing, Article Advanced Drug Delivery Reviews ; Volume 123 , 2018 , Pages 33-64 ; 0169409X (ISSN) ; Sahandi Zangabad, P ; Moosavi Basri, S. M ; Sahandi Zangabad, K ; Ghamarypour, A ; Aref, A. R ; Karimi, M ; Hamblin, M. R ; Sharif University of Technology
Elsevier B.V 2018
According to the latest report from the World Health Organization, an estimated 265,000 deaths still occur every year as a direct result of burn injuries. A widespread range of these deaths induced by burn wound happens in low- and middle-income countries, where survivors face a lifetime of morbidity. Most of the deaths occur due to infections when a high percentage of the external regions of the body area is affected. Microbial nutrient availability, skin barrier disruption, and vascular supply destruction in burn injuries as well as systemic immunosuppression are important parameters that cause burns to be susceptible to infections. Topical antimicrobials and dressings are generally...
Article International Journal of Medical Robotics and Computer Assisted Surgery ; Volume 14, Issue 1 , February , 2018 ; 14785951 (ISSN) ; Farahmand, F ; Vossoughi, G ; Ghavidel, A. A ; Rezayat, M ; Sharif University of Technology
John Wiley and Sons Ltd 2018
Background: Accurate tracking of the heart surface motion is a major requirement for robot assisted beating heart surgery. Method: The feasibility of a stereo infrared tracking system for measuring the free beating heart motion was investigated by experiments on a heart motion simulator, as well as model surgery on a dog. Results: Simulator experiments revealed a high tracking accuracy (81 μm root mean square error) when the capturing times were synchronized and the tracker pointed at the target from a 100 cm distance. The animal experiment revealed the applicability of the infrared tracker with passive markers in practical heart surgery conditions. Conclusion: With the current technology,...
Temporary skin grafts based on hybrid graphene oxide-natural biopolymer nanofibers as effective wound healing substitutes: pre-clinical and pathological studies in animal models, Article Journal of Materials Science: Materials in Medicine ; Volume 28, Issue 5 , 2017 , 73 ; 09574530 (ISSN) ; Eslahi, N ; Mehdipour, A ; Mohammadi, M ; Akbari, M ; Samadikuchaksaraei, A ; Simchi, A ; Sharif University of Technology
Springer New York LLC 2017
Abstract: In recent years, temporary skin grafts (TSG) based on natural biopolymers modified with carbon nanostructures have received considerable attention for wound healing. Developments are required to improve physico-mechanical properties of these materials to match to natural skins. Additionally, in-deep pre-clinical examinations are necessary to ensure biological performance and toxicity effect in vivo. In the present work, we show superior acute-wound healing effect of graphene oxide nanosheets embedded in ultrafine biopolymer fibers (60 nm) on adult male rats. Nano-fibrous chitosan-based skin grafts crosslinked by Genepin with physico-mechanical properties close to natural skins were...
On the biological performance of graphene oxide-modified chitosan/polyvinyl pyrrolidone nanocomposite membranes: In vitro and in vivo effects of graphene oxide, Article Materials Science and Engineering C ; Volume 70 , 2017 , Pages 121-131 ; 09284931 (ISSN) ; Simchi, A ; Sharif University of Technology
Elsevier Ltd 2017
Nanofibrous structures that mimic the native extracellular matrix and promote cell adhesion have attracted considerable interest for biomedical applications. In this study, GO-modified nanofibrous biopolymers (GO) were prepared by electrospinning blended solutions of chitosan (80 vol%), polyvinyl pyrrolidone (15 vol%), polyethylene oxide (5 vol%) containing GO nanosheets (0–2 wt%). It is shown that GO nanosheets significantly change the conductivity and viscosity of highly concentrated chitosan solutions, so that ultrafine and uniform fibers with an average diameter of 60 nm are spinnable. The GO-reinforced nanofibers with controlled pore structure exhibit enhanced elastic modulus and...
Article Biotechnology Letters ; Volume 35, Issue 8 , August , 2013 , Pages 1191-1197 ; 01415492 (ISSN) ; Keyhanvar, N ; Kalhor, H. R ; Rezaei, A ; Sharif University of Technology
Hepcidin is a peptide hormone that plays an important role in iron metabolism. We have produced a recombinant mouse hepcidin-1 by using baculovirus expression system. Its expression yield was 25 μg/ml when cell culture media were supplemented with a protease inhibitor cocktail. The recombinant mouse hepcidin-1 and synthetic human hepcidin-25 had similar effects on reducing ferroportin expression in J774A cell line and in peritoneal macrophages. However, synthetic human hepcidin-25 was more efficient than recombinant mouse hepcidin-1 in reducing iron concentration in blood circulation (p < 0.01)
Article Epilepsy and Behavior ; Volume 27, Issue 2 , 2013 , Pages 355-364 ; 15255050 (ISSN) ; Mousavi, S. R ; Motaghi, S ; Dehghani, A ; Vosoughi Vahdat, B ; Shamsollahi, M. B ; Sayyah, M ; Noorbakhsh, S. M ; Sharif University of Technology
Objective: Epileptic seizure detection is a key step for epilepsy assessment. In this work, using the pentylenetetrazole (PTZ) model, seizures were induced in rats, and ECoG signals in interictal, preictal, ictal, and postictal periods were recorded. The recorded ECoG signals were then analyzed to detect epileptic seizures in the epileptic rats. Methods: Two different approaches were considered in this work: thresholding and classification. In the thresholding approach, a feature is calculated in consecutive windows, and the resulted index is tracked over time and compared with a threshold. The moment the index crosses the threshold is considered as the moment of seizure onset. In the...
Application of a dissimilarity index of EEG and its sub-bands on prediction of induced epileptic seizures from rat's EEG signals, Article IRBM ; Volume 33, Issue 5-6 , December , 2012 , Pages 298-307 ; 19590318 (ISSN) ; Mousavi, S. R ; Shamsollahi, M. B ; Vosoughi Vahdat, B ; Sayyah, M ; Motaghi, S ; Dehghani, A ; Noorbakhsh, S. M ; Sharif University of Technology
Objective: Epileptic seizures are defined as manifest of excessive and hyper-synchronous activity of neurons in the cerebral cortex that cause frequent malfunction of the human central nervous system. Therefore, finding precursors and predictors of epileptic seizure is of utmost clinical relevance to reduce the epileptic seizure induced nervous system malfunction consequences. Researchers for this purpose may even guide us to a deep understanding of the seizure generating mechanisms. The goal of this paper is to predict epileptic seizures in epileptic rats. Methods: Seizures were induced in rats using pentylenetetrazole (PTZ) model. EEG signals in interictal, preictal, ictal and postictal...
Evaluation of radiogallium-labeled, folate-embedded superparamagnetic nanoparticles in fibrosarcoma-bearing mice, Article Journal of Cancer Research and Therapeutics ; Volume 8, Issue 2 , 2012 , Pages 204-208 ; 09731482 (ISSN) ; Jalilian, A.R ; Yousefnia, H ; Shafaii, K ; Pouladian, M ; Mahmoudi, M ; Sharif University of Technology
Context: Elevated expression of the folate receptor (FR) occurs in many human malignancies. Thus, folate targeting is widely utilized in drug delivery purposes specially using nano-radioactive agents. Aims: In this work, we report production and biological evaluation of gallium-67 labeled superparamagnetic iron oxide nanoparticles, embedded by folic acid (67 Ga-SPION-folate) complex especially in tumor-bearing mice for tumor imaging studies. Settings and Design: The structure of SPION-folate was confirmed by X-ray diffraction (XRD), transmission electron microscopy (TEM) and foureir transform infrared spectroscopy (FT-IR) analyses. The radiolabeled SPION-folate formation was confirmed by...
Alterations of the electroencephalogram sub-bands amplitude during focal seizures in the pilocarpine model of epilepsy, Article Physiology and Pharmacology ; Volume 16, Issue 1 , 2012 , Pages 11-20 ; 17350581 (ISSN) ; Niknazar, M ; Sayyah, M ; babapour, V ; Vahdat, B. V ; Shamsollahi, M. B ; Sharif University of Technology
Introduction: Temporal lobe epilepsy (TLE) is the most common and drug resistant epilepsy in adults. Due to behavioral, morphologic and electrographic similarities, pilocarpine model of epilepsy best resembles TLE. This study was aimed at determination of the changes in electroencephalogram (EEG) sub-bands amplitude during focal seizures in the pilocarpine model of epilepsy. Analysis of these changes might help detection of a pre-seizure state before an oncoming seizure. Methods: Rats were treated by scopolamine (1mg/kg, s.c) to prevent cholinergic effects. After 30 min, pilocarpine (380 mg/kg, i.p) was administered to induce status epilepticus (SE) and 2 hours after SE, diazepam (20 mg/kg,...
Early detection of immunization: A study based on an animal model using 1H nuclear magnetic resonance spectroscopy, Article Pakistan Journal of Biological Sciences ; Volume 14, Issue 3 , 2011 , Pages 195-203 ; 10288880 (ISSN) ; Arjmand, M ; Tafazzoli, M ; Ghohzadeh, A ; Pourfallah, F ; Sadeghi, S ; Mirzazadeh, R ; Mirkham, F ; Tahen, S ; Iravam, A ; Bayat, P ; Vahabi, F ; Sharif University of Technology
Vaccines require a period of at least three months for clinical trials, hence a method that can identify elicitation of immune response a few days after the first dose is a necessity. Evolutionary variable selections are modeling approaches for proper manipulation of available data which were used to set up an animal model for classification of time dependent 'HNMR metabolomic profiles and pattern recognition of fluctuations of metabolites in two groups of male rabbits. One group of rabbits was immunized with human red blood cells and the other used as control. Blood was obtained every 48 h from each rabbit for a period of six weeks and the serum monitored for antibodies and metabolites by...
Comparison of transplantation of bone marrow stromal cells (BMSC) and stem cell mobilization by granulocyte colony stimulating factor after traumatic brain injury in rat, Article Iranian Biomedical Journal ; Volume 14, Issue 4 , Oct , 2010 , Pages 142-149 ; 1028852X (ISSN) ; Marzban, M ; Mehdizadeh, M ; Joghataei, M. T ; Khoei, S ; Pirhajati Mahabadi, V ; Laribi, B ; Tondar, M ; Moshkforoush, A ; Sharif University of Technology
Background: Recent clinical studies of treating traumatic brain injury (TBI) with autologous adult stem cells led us to compare effect of intravenous injection of bone marrow mesenchymal stem cells (BMSC) and bone marrow hematopoietic stem cell mobilization, induced by granulocyte colony stimulating factor (G-CSF), in rats with a cortical compact device. Methods: Forty adult male Wistar rats were injured with controlled cortical impact device and divided randomly into four groups. The treatment groups were injected with 2 × 106 intravenous bone marrow stromal stem cell (n = 10) and also with subcutaneous G-CSF (n = 10) and sham-operation group (n = 10) received PBS and "bromodeoxyuridine...
The effect of temperature on eccentric contraction-induced isometric force loss in isolated perfused rat medial gastrocnemius muscle, Article Tehran University Medical Journal ; Volume 66, Issue 6 , 2008 , Pages 388-395 ; 16831764 (ISSN) ; Keshavarz, M ; Gharibzadeh, S ; Marvi, H ; Mosayebnejad, J ; Ebrahimi Takamjani, E ; Sharif University of Technology
Tehran University of Medical Sciences 2008
Background: The typical features of eccentric exercise-induced muscle damage are delayed-onset muscle soreness (DOMS) and prolonged loss of muscle strength. It has been shown that passive warmth is effective in reducing muscle injury. Due to the interaction of different systems in vivo, we used isolated perfused medial gastrocnemius skeletal muscle to study the direct effect of temperature on the eccentric contraction-induced force loss. Methods: After femoral artery cannulation of a rat, the left medial gastrocnemius muscle was separated and then the entire lower limb was transferred into a prewarmed (35oC) chamber. With the chamber temperature at 31, 35 and 39oC before and during eccentric...
An improved synthesis and preliminary biodistribution study of a technetium-99m-labeled2-amino-2-deoxy(thioacetyl)-D-glucose complex ([ 99mTc]-TA-DG) as a tumor imaging agent, Article Iranian Journal of Nuclear Medicine ; Volume 15, Issue 28 , 2007 , Pages 43-48 ; 16812824 (ISSN) ; Sadeghzadeh, M ; Charkhlooie, G ; Haghir Ebrahimabadi, K ; Saeedi, M. R ; Sharif University of Technology
Introduction: This report describes the synthesis of 2-Amino-2-deoxy(S- benzoylthioacetyl)-D-glucose (S-Bz-TA-DG), radiolabeled with [ 99mTc(CO)3(OH2)3]+ complex with a procedure including deprotection of the benzoyl group, characterization by HPLC using a C18 reverse phase column and preliminary biodistribution study in normal mice. Methods: [99mTc(CO) 3(H2O)3]+ complex was used to label TA-DG with 99mTc. This complex was prepared using up to 46 mCi of Na99mTcO4 in 1mL saline. The radiochemical purity (>95%) was determined by TLC in normal saline solution as the mobile phase. Radio-HPLC analysis of [99mTc]-(TA-DG) at pH=9.5-10, revealed that labeling with 99mTc resulted in the formation of...
Article Journal of Biomedical Science ; Vol. 21, issue. 1 , July , 2014 ; ISSN: 10217770 ; Ghaemi, A ; Gorji, A ; Kalhor, H. R ; Sajadian, A ; Tabarraei, A ; Moradi, A ; Atyabi, F ; Kelishadi, M ; Sharif University of Technology
Cervical cancer is the second-most-common cause of malignancies in women worldwide, and the oncogenic activity of the human papilloma virus types (HPV) E7 protein has a crucial role in anogenital tumors. In this study, we have designed a therapeutic vaccine based on chitosan nanodelivery systems to deliver HPV-16 E7 DNA vaccine, considered as a tumor specific antigen for immunotherapy of HPV-associated cervical cancer. We have developed a Nano-chitosan (NCS) as a carrier system for intramuscular administration using a recombinant DNA vaccine expressing HPV-16 E7 (NCS-DNA E7 vaccine). NCS were characterized in vitro for their gene transfection ability. Results: The transfection of CS-pEGFP...
Disc size markedly influences concentration profiles of intravenously administered solutes in the intervertebral disc: A computational study on glucosamine as a model solute, Article European Spine Journal ; Vol. 23, issue. 4 , April , 2014 , p. 715-723 ; Shirazi-Adl, A ; Parnianpour, M ; Urban, J. P. G ; Sharif University of Technology
Purpose: Tests on animals of different species with large differences in intervertebral disc size are commonly used to investigate the therapeutic efficacy of intravenously injected solutes in the disc. We hypothesize that disc size markedly affects outcome. Methods: Here, using a small non-metabolized molecule, glucosamine (GL) as a model solute, we calculate the influence of disc size on transport of GL into rat, rabbit, dog and human discs for 10 h post intravenous-injection. We used transient finite element models and considered an identical GL supply for all animals. Results: Huge effects of disc size on GL concentration profiles were found. Post-injection GL concentration in the rat...
کلیدواژه های تکراریCurcumin loading potentiates the neuroprotective efficacy of Fe3O4 magnetic nanoparticles in cerebellum cells of schizophrenic rats, Article Biomedicine and Pharmacotherapy ; Volume 108 , 2018 , Pages 1244-1252 ; 07533322 (ISSN) ; Ashrafi Hafez, A ; Abdorahim, M ; Abdollahifar, M. A ; Shabani, R ; Peirovi, H ; Simchi, A ; Ashtari, K ; Sharif University of Technology
Background: The aim of this study was to investigate the neurotoxic effects of Fe3O4 magnetic- CurNPs on isolated schizophrenia mitochondria of rats as an in vivo model. Methods: We designed CMN loaded superparamagnetic iron oxide nanoparticles (SPIONs) (Fe3O4 magnetic- CurNPs) to achieve an enhanced therapeutic effect. The physicochemical properties of Fe3O4 magnetic- CurNPs were characterized using X-ray diffraction (XRD), and dynamic laser light scattering (DLS) and zeta potential. Further, to prove Fe3O4 magnetic- CurNPs results in superior therapeutic effects, and also, the mitochondrial membrane potential collapse, mitochondrial complex II activity, reactive oxygen species generation,...