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The different fate of satellite cells on conductive composite electrospun nanofibers with graphene and graphene oxide nanosheets
, Article Biomedical Materials (Bristol) ; Volume 11, Issue 2 , 2016 ; 17486041 (ISSN) ; Soleimani, M ; Hatamie, S ; Zamanlui, S ; Ranjbarvan, P ; Vossoughi, M ; Hosseinzadeh, S ; Sharif University of Technology
Institute of Physics Publishing
2016
Abstract
Electrospinning of composite polymer solutions provides fantastic potential to prepare novel nanofibers for use in a variety of applications. The addition of graphene (G) and graphene oxide (GO) nanosheets to bioactive polymers was found to enhance their conductivity and biocompatibility. Composite conductive nanofibers of polyaniline (PANI) and polyacrylonitrile (PAN) with G and GO nanosheets were prepared by an electrospinning process. The fabricated membranes were investigated by physical and chemical examinations including scanning electron microscopy (SEM), Raman spectroscopy, x-ray diffraction (XRD) and tensile assay. The muscle satellite cells enriched by a pre-plating technique were...
Study of epithelial differentiation and protein expression of keratinocyte-mesenchyme stem cell co-cultivation on electrospun nylon/B. vulgaris extract composite scaffold
, Article Materials Science and Engineering C ; Volume 75 , 2017 , Pages 653-662 ; 09284931 (ISSN) ; Soleimani, M ; Vossoughi, M ; Ranjbarvan, P ; Hamedi, S ; Zamanlui, S ; Mahmoudifard, M ; Sharif University of Technology
Abstract
Employing of the composite electrospun scaffold containing herbal extract in conjugation with co-culturing of cells can open up new window to the design of efficient biomaterials for skin tissue regeneration. Here, we introduce the synergistic effect of composite electrospun nanofibrous scaffold of nylon66 loaded with Beta vulgaris (B. vulgaris) (extract of beet roots, a plants whose widely used in Iranian folk medicine as wound healing medicine) and co-culture of mesenchymal stem-cells (MSCs)-human keratinocyte (H-keratino) differentiation towards epithelial lineage. In vitro biocompatibility was examined through MTT assay and epithelial differentiation checked by real-time PCR and...
Two schemes for production of biosurfactant from Pseudomonas aeruginosa MR01: Applying residues from soybean oil industry and silica sol–gel immobilized cells
, Article Colloids and Surfaces B: Biointerfaces ; Volume 152 , 2017 , Pages 159-168 ; 09277765 (ISSN) ; Ebadipour, N ; Roostaazad, R ; Partovi, M ; Bahmaei, M ; Sharif University of Technology
Elsevier B.V
2017
Abstract
Rhamnolipids are the most common biosurfactants and P. aeruginosa strains are the most frequently studied microorganisms for the production of rhamnolipids. Eco-friendly advantages and promising applications of rhamnolipids in various industries are the major reasons for pursuing the economic production of these biosurfactants. This study shows that cultivation of P. aeruginosa MR01 in medium contained inexpensive soybean oil refinery wastes which exhibited similar levels and homologues of rhamnolipids. Mass spectrometry indicated that the Rha-C10-C10 and Rha-Rha-C10-C10 constitute the main rhamnolipids in different cultures of MR01 including one of oil carbon source analogues. Moreover,...
Theoretical modeling of actin-retrograde-flow passing clusters of confined T cell receptors
, Article Mathematical Biosciences ; Volume 283 , 2017 , Pages 1-6 ; 00255564 (ISSN) ; Firoozabadi, B ; Saidi, M. S ; Sharif University of Technology
Elsevier Inc
2017
Abstract
Through the activation process of T cells, actin filaments move from the cell periphery toward the cell center. The moving filaments engage with T cell receptors and thus contribute to transportation of the signaling molecules. To study the connection between the moving actin filaments and T cell receptors, an experiment available in the literature has measured filaments flow velocity passing over a region of confined clusters of receptors. It shows that flow velocity decreases in the proximity of the receptors, and then regains its normal value after traversing the region, suggesting a dissipative friction-like connection. In this work, we develop a minimal theoretical model to re-examine...
Cell shape affects nanoparticle uptake and toxicity: An overlooked factor at the nanobio interfaces
, Article Journal of Colloid and Interface Science ; Volume 531 , 2018 , Pages 245-252 ; 00219797 (ISSN) ; Ghahremani, M. H ; Hashemi, F ; Hormozi Nezhad, M. R ; Raoufi, M ; Zanganeh, S ; Atyabi, F ; Dinarvand, R ; Mahmoudi, M ; Sharif University of Technology
Academic Press Inc
2018
Abstract
Hypothesis: It is now being increasingly accepted that cells in their native tissue show different morphologies than those grown on a culture plate. Culturing cells on the conventional two-dimensional (2D) culture plates does not closely resemble the in vivo three-dimensional (3D) structure of cells which in turn seems to affect cellular function. This is one of the reasons, among many others, that nanoparticles uptake and toxicology data from 2D culture plates and in vivo environments are not correlated with one another. In this study, we offer a novel platform technology for producing more in vivo-like models of in vitro cell culture. Experiments: The normal fibroblast cells (HU02) were...
Magnetic carbon nanotubes: preparation, physical properties, and applications in biomedicine
, Article Artificial Cells, Nanomedicine and Biotechnology ; Volume 46, Issue 7 , 2018 , Pages 1314-1330 ; 21691401 (ISSN) ; Farshbaf, M ; Annabi, N ; Kavetskyy, T ; Khalilov, R ; Saghfi, S ; Akbarzadeh, A ; Mousavi, S ; Sharif University of Technology
Abstract
Magnetic carbon nanotubes (MCNTs) have been widely studied for their potential applications in medicine, diagnosis, cell biology, analytical chemistry, and environmental technology. Introduction of MCNTs paved the way for the emergence of new approaches in nanobiotechnology and biomedicine as a result of their multifarious properties embedded within either the carbon nanotubes (CNTs) or magnetic parts. Numerous preparation techniques exists for functionalizing CNTs with magnetic nanoparticles, and these versatile strategies lay the ground for the generation of novel and versatile systems which are applicable to many industries and biological areas. Here, we review and discuss the recent...
Novel fluoridated silk fibroin/ TiO2 nanocomposite scaffolds for bone tissue engineering
, Article Materials Science and Engineering C ; Volume 82 , 2018 , Pages 265-276 ; 09284931 (ISSN) ; Madaah Hosseini, H. R ; Samadikuchaksaraei, A ; Sharif University of Technology
Elsevier Ltd
2018
Abstract
It is known that Fluoride ions strongly affect bone mineralization and formation. In the present study, the engineered bone tissue scaffolds are fabricated using silk fibroin (SF) and flouridated TiO2 nanoparticles. TiO2 nanoparticles are modified by fluoride ions, and different levels (0, 5, 10, 15 and 20 wt%) of the fluoridated TiO2 nanoparticles (TiO2-F) were subsequently added to the SF matrix through phase separation method to prepare silk fibroin/flouridated TiO2 nanocomposite scaffolds (SF/TiO2-F). Phase structure, functional groups, morphology and mechanical properties of the obtained scaffolds were evaluated by X-ray diffraction method (XRD), Fourier transform infrared spectroscopy...
A microfabricated platform for the study of chondrogenesis under different compressive loads
, Article Journal of the Mechanical Behavior of Biomedical Materials ; Volume 78 , 2018 , Pages 404-413 ; 17516161 (ISSN) ; Jahanbakhsh, A ; Saidi, M. S ; Bonakdar, S ; Sharif University of Technology
Elsevier Ltd
2018
Abstract
Microfluidic devices are beneficial in miniaturizing and multiplexing various cellular assays in a single platform. Chondrogenesis is known to pertain to chemical, topographical, and mechanical cues in the microenvironment. Mechanical cues themselves have numerous parameters such as strain magnitude, frequency, and stimulation time. Effects of different strain magnitudes on the chondrogenic differentiation of adult stem cells have not been explored thoroughly. Here, a new multilayer microdevice is presented for the unidirectional compressive stimulation of cells in a three-dimensional cell culture. Numerical simulations were performed to evaluate and optimize the design. Results showed a...
Fully-coupled mathematical modeling of actomyosin-cytosolic two-phase flow in a highly deformable moving Keratocyte cell
, Article Journal of Biomechanics ; Volume 67 , January , 2018 , Pages 37-45 ; 00219290 (ISSN) ; Firoozabadi, B ; Saidi, M. S ; Sharif University of Technology
Elsevier Ltd
2018
Abstract
Interaction between intracellular dynamics and extracellular matrix (ECM) generally occurred into very thin fragment of moving cell, namely lamellipodia, enables all movable cells to crawl on ECM. In fast-moving cells such as fish Keratocytes, Lamellipodia including most cell area finds a fan-like shape during migration, with a variety of aspect ratio function of fish type. In this work, our purpose is to present a novel and more complete two-dimensional continuum mathematical model of actomyosin-cytosolic two-phase flow of a self-deforming Keratocyte with circular spreaded to steady fan-like shape. In the new approach, in addition to the two-phase flow of the F-actin and cytosol, the...
Natural compounds for skin tissue engineering by electrospinning of nylon-Beta vulgaris
, Article ASAIO Journal ; Volume 64, Issue 2 , 2018 , Pages 261-269 ; 10582916 (ISSN) ; Mahmoudifard, M ; Kehtari, M ; Babaie, A ; Hamedi, S ; Mirzaei, S ; Soleimani, M ; Hosseinzadeh, S ; Sharif University of Technology
Lippincott Williams and Wilkins
2018
Abstract
Natural compounds containing polysaccharide ingredients have been employed as candidates for treatment of skin tissue. Herein, for the first time, electrospinning setup was proposed to fabricate an efficient composite nanofibrous structure of Beta vulgaris (obtained from Beet [Chenopodiaceae or Amaranthaceae]) belonged to polysaccharides and an elastic polymer named nylon 66 for skin tissue engineering. Both prepared scaffolds including noncomposite and composite types were studied by Scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy, mechanical assay, and contact angle. Scanning electron microscope examinations have approved the uniform and homogeneous...
Antibacterial properties of nanoporous graphene oxide/cobalt metal organic framework
, Article Materials Science and Engineering C ; Volume 104 , 2019 ; 09284931 (ISSN) ; Ahadian, M. M ; Soufi Zomorod, M ; Torabi, S ; Babaie, A ; Hosseinzadeh, S ; Soleimani, M ; Hatami, N ; Wei, Z. H ; Sharif University of Technology
Elsevier Ltd
2019
Abstract
Metal-organic framework (MOF) based graphene oxide (GO) recently merits of attention because of the relative correspondence of GO with metal ions and organic binding linkers. Furthermore, introducing the GO to the Co-MOF to make a new nanoporous hybrid have are improved the selectivity and stability of the Co-MOF. Here the graphene oxide/cobalt metal organic framework (GO/Co-MOF) was synthesized by a solvothermal process using cobalt salt and terephthalic acid and used for biocidal activity, against the growth of the Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria. X-ray diffraction, Fourier transform infrared spectroscopy and Raman spectroscopy were confirmed...
Phenomenological tissue fracture modeling for an Endoscopic Sinus and Skull Base Surgery training system based on experimental data
, Article Medical Engineering and Physics ; Volume 68 , 2019 , Pages 85-93 ; 13504533 (ISSN) ; Farahmand, F ; Vossoughi, G ; Moradi, H ; Mousa Sadr Hosseini, S ; Sharif University of Technology
Elsevier Ltd
2019
Abstract
The ideal simulator for Endoscopic Sinus and Skull Base Surgery (ESSS)training must be supported by a physical model and provide repetitive behavior in a controlled environment. Development of realistic tissue models is a key part of ESSS virtual reality (VR)-based surgical simulation. Considerable research has been conducted to address haptic or force feedback and propose a phenomenological tissue fracture model for sino-nasal tissue during surgical tool indentation. Mechanical properties of specific sino-nasal regions of the sheep head have been studied in various indentation and relaxation experiments. Tool insertion at different indentation rates into coronal orbital floor (COF)tissue is...
Fabrication, modeling and optimization of lyophilized advanced platelet rich fibrin in combination with collagen-chitosan as a guided bone regeneration membrane
, Article International Journal of Biological Macromolecules ; Volume 125 , 2019 , Pages 383-391 ; 01418130 (ISSN) ; Mashayekhan, S ; Saadatmand, M ; Sharif University of Technology
Elsevier B.V
2019
Abstract
In this study, lyophilized advanced platelet rich fibrin (A-PRF) was used in combination with collagen-chitosan membrane for the first time to combine advantages of both collagen and A-PRF membranes. Response surface methodology (RSM) was used to design the experimental condition and to correlate the effects of parameters, including chitosan/collagen (chit/col) weight ratio and A-PRF concentration on Young's modulus, mesenchymal stem cell (MSCs) viability and degradation rate of the membranes. Results showed that Young's modulus of the membranes was intensified by increasing chit/col weight ratio and decreasing A-PRF concentration from 3 to 8 MPa. Cell viability of MSCs was improved by both...
Bilayered heparinized vascular graft fabricated by combining electrospinning and freeze drying methods
, Article Materials Science and Engineering C ; Volume 94 , 2019 , Pages 1067-1076 ; 09284931 (ISSN) ; Shamloo, A ; Sharif University of Technology
Elsevier Ltd
2019
Abstract
Small diameter vascular grafts (<6 mm) are highly demanded for patients suffering from severe occluded arteries to be used as a bypass or substituted conduit. Fabricating a graft with appropriate structural, mechanical and cell growth properties which has simultaneously anti-thrombogenic trait is a challenge nowadays. Here, we proposed a bilayer heparinized vascular graft that can mimic the structural and mechanical characteristics close to those of the native coronary artery by combining electrospinning and freeze drying methods. In this study, the inner layer was made by co-electrospinning of synthetic polymer, poly-caprolactone (PCL) and the natural polymer, gelatin (Gel). Also, heparin...
Bilayered heparinized vascular graft fabricated by combining electrospinning and freeze drying methods
, Article Materials Science and Engineering C ; Volume 94 , 2019 , Pages 1067-1076 ; 09284931 (ISSN) ; Shamloo, A ; Sharif University of Technology
Elsevier Ltd
2019
Abstract
Small diameter vascular grafts (<6 mm) are highly demanded for patients suffering from severe occluded arteries to be used as a bypass or substituted conduit. Fabricating a graft with appropriate structural, mechanical and cell growth properties which has simultaneously anti-thrombogenic trait is a challenge nowadays. Here, we proposed a bilayer heparinized vascular graft that can mimic the structural and mechanical characteristics close to those of the native coronary artery by combining electrospinning and freeze drying methods. In this study, the inner layer was made by co-electrospinning of synthetic polymer, poly-caprolactone (PCL) and the natural polymer, gelatin (Gel). Also, heparin...
Graphene oxide negatively regulates cell cycle in embryonic fibroblast cells
, Article International Journal of Nanomedicine ; Volume 15 , 2020 , Pages 6201-6209 ; Akhavan, O ; Shamsara, M ; Ansari Majd, S ; Sanati, M. H ; Daliri Joupari, M ; Farmany, A ; Sharif University of Technology
Dove Medical Press Ltd
2020
Abstract
Background: Unique properties of graphene and its derivatives make them attractive in the field of nanomedicine. However, the mass application of graphene might lead to side effects, which has not been properly addressed in previous studies, especially with regard to its effect on the cell cycle. Methods: The effect of two concentrations (100 and 200 μg/mL) of nano-and microsized graphene oxide (nGO and mGO) on apoptosis, cell cycle, and ROS generation was studied. The effect of both sizes on viability and genotoxicity of the embryonic fibroblast cell cycle was evaluated. MTT and flow cytometry were applied to evaluate the effects of graphene oxide (GO) nanosheets on viability of cells....
MicroRNA profiling reveals important functions of miR-125b and let-7a during human retinal pigment epithelial cell differentiation
, Article Experimental Eye Research ; Volume 190 , 2020 ; Satarian, L ; Moradi, S ; Sharifi Zarchi, A ; Günther, S ; Kamal, A ; Totonchi, M ; Mowla, S. J ; Braun, T ; Baharvand, H ; Sharif University of Technology
Academic Press
2020
Abstract
Retinal pigment epithelial (RPE) cells are indispensable for eye organogenesis and vision. To realize the therapeutic potential of in vitro-generated RPE cells for cell-replacement therapy of RPE-related retinopathies, molecular mechanisms of RPE specification and maturation need to be investigated. So far, many attempts have been made to decipher the regulatory networks involved in the differentiation of human pluripotent stem cells into RPE cells. Here, we exploited a highly-efficient RPE differentiation protocol to determine global expression patterns of microRNAs (miRNAs) during human embryonic stem cell (hESC) differentiation into RPE using small RNA sequencing. Our results revealed a...
Biohybrid oxidized alginate/myocardial extracellular matrix injectable hydrogels with improved electromechanical properties for cardiac tissue engineering
, Article International Journal of Biological Macromolecules ; Volume 180 , 2021 , Pages 692-708 ; 01418130 (ISSN) ; Mashayekhan, S ; Baheiraei, N ; Pourjavadi, A ; Sharif University of Technology
Elsevier B.V
2021
Abstract
Injectable hydrogels which mimic the physicochemical and electromechanical properties of cardiac tissue is advantageous for cardiac tissue engineering. Here, a newly-developed in situ forming double-network hydrogel derived from biological macromolecules (oxidized alginate (OA) and myocardial extracellular matrix (ECM)) with improved mechanical properties and electrical conductivity was optimized. 3-(2-aminoethyl amino) propyltrimethoxysilane (APTMS)-functionalized reduced graphene oxide (Amine-rGO) was added to this system with varied concentrations to promote electromechanical properties of the hydrogel. Alginate was partially oxidized with an oxidation degree of 5% and the resulting OA...
Tissue growth into three-dimensional composite scaffolds with controlled micro-features and nanotopographical surfaces
, Article Journal of Biomedical Materials Research - Part A ; Volume 101, Issue 10 , 2013 , Pages 2796-2807 ; 15493296 (ISSN) ; Simchi, A ; Dunlop, J. W. C ; Fratzl, P ; Bagheri, R ; Vossoughi, M ; Sharif University of Technology
2013
Abstract
Controlling topographic features at all length scales is of great importance for the interaction of cells with tissue regenerative materials. We utilized an indirect three-dimensional printing method to fabricate polymeric scaffolds with pre-defined and controlled external and internal architecture that had an interconnected structure with macro- (400-500 μm) and micro- (∼25 μm) porosity. Polycaprolactone (PCL) was used as model system to study the kinetics of tissue growth within porous scaffolds. The surface of the scaffolds was decorated with TiO2 and bioactive glass (BG) nanoparticles to the better match to nanoarchitecture of extracellular matrix (ECM). Micrometric BG particles were...
Manipulating failure mechanism of rapid prototyped scaffolds by changing nodal connectivity and geometry of the pores
, Article Journal of Biomechanics ; Volume 45, Issue 16 , 2012 , Pages 2866-2875 ; 00219290 (ISSN) ; Bagheri, R ; Zehtab Yazdi, A ; Sharif University of Technology
2012
Abstract
The performance of cellular solids in biomedical applications relies strongly on a detailed understanding of the effects of pore topology on mechanical properties. This study aims at characterizing the failure mechanism of scaffolds based on nodal connectivity (number of struts that meet in joints) and geometry of the pores. Plastic models of scaffolds having the same relative density but different cubic and trigonal unit cells were designed and then fabricated via three dimensional (3-D) printing. Unit cells were repeated in different arrangements in 3-D space. An in-situ imaging technique was utilized to study the progressive deformation of the scaffold models. Different nodal...