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extracellular-matrix
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Total 52 records
A cellular cardiac matrix-based porous microcarrier as a cell delivery system in myocardial tissue engineering application
, Article Iranian Polymer Journal (English Edition) ; Volume 31, Issue 9 , 2022 , Pages 1079-1091 ; 10261265 (ISSN) ; Mashayekhan, S ; Khanmohammadi, M ; Sharif University of Technology
Springer Science and Business Media Deutschland GmbH
2022
Abstract
Myocardial infarction (MI) causes a high mortality rate in the world every year. Myocardial tissue engineering using extracellular matrix-derived substrate and cytocompatible biopolymers is a promising approach for treating MI. Besides, injectable porous microspheres are developing engineer constructs to use as dual-purpose microcarriers for cell culture and injectable scaffolds in trivial invasiveness for tissue implantation. This study aimed to fabricate porous microcarriers composed of myocardial extracellular matrix and chitosan using an electrospraying technique. The effect of electrospraying parameters, including extracellular matrix/chitosan ratio and voltage, on MCs diameter was...
Controlling differentiation of stem cells for developing personalized organ-on-chip platforms
, Article Advanced Healthcare Materials ; Volume 7, Issue 2 , 2018 ; 21922640 (ISSN) ; Jafari, P ; Sheikh Hassani, M ; Heidary Araghi, B ; Mohammadi, M. H ; Ghafari, A. M ; Hassanpour Tamrin, S ; Pezeshgi Modarres, H ; Rezaei Kolahchi, A ; Ahadian, S ; Sanati Nezhad, A ; Sharif University of Technology
Wiley-VCH Verlag
2018
Abstract
Organ-on-chip (OOC) platforms have attracted attentions of pharmaceutical companies as powerful tools for screening of existing drugs and development of new drug candidates. OOCs have primarily used human cell lines or primary cells to develop biomimetic tissue models. However, the ability of human stem cells in unlimited self-renewal and differentiation into multiple lineages has made them attractive for OOCs. The microfluidic technology has enabled precise control of stem cell differentiation using soluble factors, biophysical cues, and electromagnetic signals. This study discusses different tissue- and organ-on-chip platforms (i.e., skin, brain, blood–brain barrier, bone marrow, heart,...
Cell shape affects nanoparticle uptake and toxicity: An overlooked factor at the nanobio interfaces
, Article Journal of Colloid and Interface Science ; Volume 531 , 2018 , Pages 245-252 ; 00219797 (ISSN) ; Ghahremani, M. H ; Hashemi, F ; Hormozi Nezhad, M. R ; Raoufi, M ; Zanganeh, S ; Atyabi, F ; Dinarvand, R ; Mahmoudi, M ; Sharif University of Technology
Academic Press Inc
2018
Abstract
Hypothesis: It is now being increasingly accepted that cells in their native tissue show different morphologies than those grown on a culture plate. Culturing cells on the conventional two-dimensional (2D) culture plates does not closely resemble the in vivo three-dimensional (3D) structure of cells which in turn seems to affect cellular function. This is one of the reasons, among many others, that nanoparticles uptake and toxicology data from 2D culture plates and in vivo environments are not correlated with one another. In this study, we offer a novel platform technology for producing more in vivo-like models of in vitro cell culture. Experiments: The normal fibroblast cells (HU02) were...
Micromechanics and constitutive modeling of connective soft tissues
, Article Journal of the Mechanical Behavior of Biomedical Materials ; Volume 60 , 2016 , Pages 157-176 ; 17516161 (ISSN) ; Ahmadian, M. T ; Firozbakhsh, K ; Aghdam, M. M ; Sharif University of Technology
Elsevier Ltd
Abstract
In this paper, a micromechanical model for connective soft tissues based on the available histological evidences is developed. The proposed model constituents i.e. collagen fibers and ground matrix are considered as hyperelastic materials. The matrix material is assumed to be isotropic Neo-Hookean while the collagen fibers are considered to be transversely isotropic hyperelastic. In order to take into account the effects of tissue structure in lower scales on the macroscopic behavior of tissue, a strain energy density function (SEDF) is developed for collagen fibers based on tissue hierarchical structure. Macroscopic response and properties of tissue are obtained using the numerical...
Construction of scaffolds composed of acellular cardiac extracellular matrix for myocardial tissue engineering
, Article Biologicals ; Volume 53 , 2018 , Pages 10-18 ; 10451056 (ISSN) ; Mashayekhan, S ; Ghanbari Asl, S ; Hajebrahimi, Z ; Sharif University of Technology
Academic Press
2018
Abstract
High rates of mortality and morbidity stemming from cardiovascular diseases unveil extreme limitations in current therapies despite enormous advances in medical and pharmaceutical sciences. Following myocardial infarction (MI), parts of myocardium undergo irreversible remodeling and is substituted by a scar tissue which eventually leads to heart failure (HF). To address this issue, cardiac patches have been utilized to initiate myocardial regeneration. In this study, a porous cardiac patch is fabricated using a mixture of decellularized myocardium extracellular matrix (ECM) and chitosan (CS). Results of rheological measurements, SEM, biodegradation test, and MTT assay showed that the...
Bioinspired nanofiber scaffold for differentiating bone marrow-derived neural stem cells to oligodendrocyte-like cells: Design, fabrication, and characterization
, Article International Journal of Nanomedicine ; Volume 15 , 2020 , Pages 3903-3920 ; Mashayekhan, S ; Abbaszadeh, H. A ; Ansarizadeh, M ; Khoramgah, M. S ; Rahimi Movaghar, V ; Sharif University of Technology
Dove Medical Press Ltd
2020
Abstract
Background: Researchers are trying to study the mechanism of neural stem cells (NSCs) differentiation to oligodendrocyte-like cells (OLCs) as well as to enhance the selective differentiation of NSCs to oligodendrocytes. However, the limitation in nerve tissue acces-sibility to isolate the NSCs as well as their differentiation toward oligodendrocytes is still challenging. Purpose: In the present study, a hybrid polycaprolactone (PCL)-gelatin nanofiber scaffold mimicking the native extracellular matrix and axon morphology to direct the differentiation of bone marrow-derived NSCs to OLCs was introduced. Materials and Methods: In order to achieve a sustained release of T3, this factor was...
Mathematical modeling of dermal wound healing: A numerical solution
, Article 2010 The 2nd International Conference on Computer and Automation Engineering, ICCAE 2010, 26 February 2010 through 28 February 2010, Singapore ; Volume 2 , 2010 , Pages 153-156 ; 9781424455850 (ISBN) ; Ghaemi Osgouie, K ; Sharif University of Technology
2010
Abstract
Though wound healing process is well-researched, this area is poorly known. One reason is that all interactions have not been discovered, the main reason, though, is that the involved processes interact in a very complicated manner with nonlinear feedback. Such complex feedback mechanisms can be easily addressed by mathematical modeling. This paper contains a review of the mathematical modeling of cell interaction with extracellular matrix components during the process of dermal wound healing with focusing on remodeling phase. The models are of partial differential equation type and solved by numerical method
Dermal wound healing-remodeling phase: A biological review
, Article 2010 The 2nd International Conference on Computer and Automation Engineering, ICCAE 2010, 26 February 2010 through 28 February 2010, Singapore ; Volume 2 , 2010 , Pages 88-90 ; 9781424455850 (ISBN) ; Ghaemi Osgouie, K ; Sharif University of Technology
2010
Abstract
Though wound healing process is well-researched, this area is poorly known. One reason is that all interactions have not been discovered, the main reason, though, is that the involved processes interact in a very complicated manner with nonlinear feedback. Such complex feedback mechanisms can be easily addressed by mathematical modeling. This paper contains a review of the mathematical modeling of cell interaction with extracellular matrix components during the process of dermal wound healing with focusing on remodeling phase. The models are of partial differential equation type and solved by finite element method
Mathematical modeling of dermal wound healing's remodeling phase: a finite element solution
, Article 2009 International Association of Computer Science and Information Technology - Spring Conference, IACSIT-SC 2009, Singapore, 17 April 2009 through 20 April 2009 ; 2009 , Pages 529-532 ; 9780769536538 (ISBN) ; Seifipour, N ; Sharif University of Technology
2009
Abstract
Though wound healing process is well-researched, this area is poorly known. One reason is that all interactions have not been discovered, the main reason, though, is that the involved processes interact in a very complicated manner with nonlinear feedback. Such complex feedback mechanisms can be easily addressed by mathematical modeling. This paper contains a review of the mathematical modeling of cell interaction with extracellular matrix components during the process of dermal wound healing with focusing on remodeling phase. The models are of partial differential equation type and solved by finite element method. © 2009 IEEE
Brain-on-a-chip: Recent advances in design and techniques for microfluidic models of the brain in health and disease
, Article Biomaterials ; Volume 285 , 2022 ; 01429612 (ISSN) ; Shamloo, A ; Nasiri, R ; de Barros, N. R ; Wang, Z. Z ; Unluturk, B. D ; Libanori, A ; Ievglevskyi, O ; Diltemiz, S. E ; Sances, S ; Balasingham, I ; Seidlits, S. K ; Ashammakhi, N ; Sharif University of Technology
Elsevier Ltd
2022
Abstract
Recent advances in biomaterials, microfabrication, microfluidics, and cell biology have led to the development of organ-on-a-chip devices that can reproduce key functions of various organs. Such platforms promise to provide novel insights into various physiological events, including mechanisms of disease, and evaluate the effects of external interventions, such as drug administration. The neuroscience field is expected to benefit greatly from these innovative tools. Conventional ex vivo studies of the nervous system have been limited by the inability of cell culture to adequately mimic in vivo physiology. While animal models can be used, their relevance to human physiology is uncertain and...
A hydrogen-bonded extracellular matrix-mimicking bactericidal hydrogel with radical scavenging and hemostatic function for ph-responsive wound healing acceleration
, Article Advanced Healthcare Materials ; Volume 10, Issue 3 , 2021 ; 21922640 (ISSN) ; Correia, A ; Hasany, M ; Figueiredo, P ; Dobakhti, F ; Eskandari, M. R ; Hosseini, S.H ; Abiri, R ; Khorshid, S ; Hirvonen, J ; Santos, H. A ; Shahbazi, M. A ; Sharif University of Technology
Wiley-VCH Verlag
2021
Abstract
Generation of reactive oxygen species, delayed blood clotting, prolonged inflammation, bacterial infection, and slow cell proliferation are the main challenges of effective wound repair. Herein, a multifunctional extracellular matrix-mimicking hydrogel is fabricated through abundant hydrogen bonding among the functional groups of gelatin and tannic acid (TA) as a green chemistry approach. The hydrogel shows adjustable physicochemical properties by altering the concentration of TA and it represents high safety features both in vitro and in vivo on fibroblasts, red blood cells, and mice organs. In addition to the merit of facile encapsulation of cell proliferation-inducing hydrophilic drugs,...
A hydrogen-bonded extracellular matrix-mimicking bactericidal hydrogel with radical scavenging and hemostatic function for ph-responsive wound healing acceleration
, Article Advanced Healthcare Materials ; Volume 10, Issue 3 , 2021 ; 21922640 (ISSN) ; Correia, A ; Hasany, M ; Figueiredo, P ; Dobakhti, F ; Eskandari, M. R ; Hosseini, S. H ; Abiri, R ; Khorshid, S ; Hirvonen, J ; Santos, H. A ; Shahbazi, M. A ; Sharif University of Technology
Wiley-VCH Verlag
2021
Abstract
Generation of reactive oxygen species, delayed blood clotting, prolonged inflammation, bacterial infection, and slow cell proliferation are the main challenges of effective wound repair. Herein, a multifunctional extracellular matrix-mimicking hydrogel is fabricated through abundant hydrogen bonding among the functional groups of gelatin and tannic acid (TA) as a green chemistry approach. The hydrogel shows adjustable physicochemical properties by altering the concentration of TA and it represents high safety features both in vitro and in vivo on fibroblasts, red blood cells, and mice organs. In addition to the merit of facile encapsulation of cell proliferation-inducing hydrophilic drugs,...