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gelatin
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Core-sheath gelatin based electrospun nanofibers for dual delivery release of biomolecules and therapeutics
, Article Materials Science and Engineering C ; Volume 108 , 2020 ; Lotfi, R ; Tamjid, E ; Shokrgozar, M. A ; Simchi, A ; Sharif University of Technology
Elsevier Ltd
2020
Abstract
Coaxial electrospinning with the ability to use simultaneously two separate solvents provides a promising strategy for drug delivery. Nevertheless, controlled release of hydrophilic and sensitive therapeutics from slow biodegradable polymers is still challenging. To address this gap, we fabricated core-sheath fibers for dual delivery of lysozyme, as a model protein, and phenytoin sodium as a small therapeutic molecule. The sheath was processed by a gelatin solution while the core fibers were fabricated from an aqueous gelatin/PVA solution. Microstructural studies by transmission and scanning electron microscopy reveal the formation of homogeneous core-sheath nanofibers with an outer and...
Biopolymeric based Interpenetrating Polymer Network for Extending the Microextraction Techniques
, M.Sc. Thesis Sharif University of Technology ; Bagheri, Habib (Supervisor)
Abstract
In this study, gelatin/polyvinylpyrrolidone semi interpenetrating polymer network scaffold was synthesized in presence of glutaraldehyde as a cross linker and a porous structure was obtained by freeze drying method. The biocompatible network with properties like huge porosity, good thermal stability and establishing different interactions, was employed as an extractive phase in needle trap device and used for evaluation of benzene, toluene, ethylbenzene and xylene (BTEX) in conjugation with gas chromatography-flame ionization detector. For investigating semi interpenetrating polymer network efficiency, Different ratios of gelatin and polyvinylpyrrolidone (gelatin/polyvinylpyrrolidone: 1:0,...
Study and Fabrication of a Multilayer Scaffold Containing Biological Agents for Skin Wounds Regeneration
, Ph.D. Dissertation Sharif University of Technology ; Alemzadeh, Iran (Supervisor) ; Vossoughi, Manouchehr (Supervisor) ; Shamloo, Amir (Co-Supervisor)
Abstract
In recent years, it is expected that the fabrication of multilayer scaffolds and the use of different methodologies in one product can be a new progressing method in skin substitute production. Accordingly, this project aims to fabricate a bilayered composite scaffold with a combination of hydrogel and electrospinning method. We have tried to prepare a scaffold made of oxidized alginate (OAL), gelatin (G), and silk fibroin (SF) without using corrosive solvents and toxic crosslinking agents as a scaffold and drug delivery system. As different biological, chemical, physical, and mechanical factors play a vital role in the healing process, we have characterized the proposed scaffold via DSC,...
Investigation and Determination of Optimal Composition of Hydrogels and Nanoparticles for Proliferation of Mesenchymal Stem Cells in Alginate Beads
, M.Sc. Thesis Sharif University of Technology ; Yaghmaei, Soheila (Supervisor) ; Arpanaei, Ayyoob (Supervisor)
Abstract
The aim of this dissertation is to investigate and determine the optimal composition of hydrogels that can meet the needs of stem cells for the growth and proliferation of mesenchymal stem cells. In this research, firstly, unfunctionalized and functionalized mesoporous silica nanoparticles (with an average diameter of approximately 90 nm), were synthesized. Scanning electron microscopy and zeta potential measurement were utilized to study their morphology and surface charge. Then, graphene oxide nanosheets were synthesized. Field emission electron microscopy, zeta potential measurement and Fourier transform infrared spectrum analysis were used to examine their morphology, surface charge and...
Design, fabrication and characterization of oxidized alginate-gelatin hydrogels for muscle tissue engineering applications
, Article Journal of Biomaterials Applications ; Volume 31, Issue 1 , 2016 , Pages 152-161 ; 08853282 (ISSN) ; Mashayekhan, S ; Fadaoddini, S ; Haghirsharifzamini, Y ; Sharif University of Technology
SAGE Publications Ltd
2016
Abstract
In this study, we reported the preparation of self cross-linked oxidized alginate-gelatin hydrogels for muscle tissue engineering. The effect of oxidation degree (OD) and oxidized alginate/gelatin (OA/GEL) weight ratio were examined and the results showed that in the constant OA/GEL weight ratio, both cross-linking density and Young's modulus enhanced by increasing OD due to increment of aldehyde groups. Furthermore, the degradation rate was increased with increasing OD probably due to decrement in alginate molecular weight during oxidation reaction facilitated degradation of alginate chains. MTT cytotoxicity assays performed on Wharton's Jelly-derived umbilical cord mesenchymal stem cells...
Shape-controlled silver NPs for shape-dependent biological activities
, Article Micro and Nano Letters ; Volume 12, Issue 9 , 2017 , Pages 647-651 ; 17500443 (ISSN) ; Yazdanpanah, A ; Abrishamkar, A ; Moztarzadeh, F ; Ramedani, A ; Pouraghaie, S ; Shirinzadeh, H ; Samadikuchaksaraei, A ; Chauhan, N. P. S ; Hopkinson, L ; Sefat, F ; Mozafari, M ; Sharif University of Technology
Abstract
The most important issue during synthesis of nanoparticles (NPs) is to avoid particle agglomeration and adhesion. There have been several attempts to use special substances such as organic surfactants, polymers and stable ligands for this purpose. In this study, silver NPs were synthesised with and without gelatin macromolecules, as a green natural biopolymer, which resulted in NPs with varying shapes and sizes. The effect of morphological characteristics on the antibacterial and antifungal properties of the synthesised NPs were studied, by comparing Gram-negative (Escherichia coli) versus Gram-positive (Staphylococcus aureus) bacteria as well as fungi (Candida albicans) by calculation of...
Porous gelatin/poly(ethylene glycol) scaffolds for skin cells
, Article Soft Materials ; Volume 15, Issue 1 , 2017 , Pages 95-102 ; 1539445X (ISSN) ; Frounchi, M ; Dadbin, S ; Sharif University of Technology
Abstract
Biocompatible porous polymeric scaffolds provide a suitable environment for proliferation of stem cells in human body. In this research work, porous gelatin–poly(ethylene glycol), PEG, based scaffolds were prepared using combination of freeze-gelation and freeze-extraction methods. Effects of various parameters such as freezing temperature, cross-linking agent, concentrations of gelatin and PEG and their blending ratio on physical and mechanical properties, swelling ratio, porosity, pore size, and degradation rate of scaffolds were investigated. Also, proliferation of fibroblast skin cells on the scaffolds was examined by MTS assay to assess the suitability of the scaffolds in wound healing...
Co-microencapsulation of lactobacillus plantarum and DHA fatty acid in alginate-pectin-gelatin biocomposites
, Article Carbohydrate Polymers ; Volume 199 , 2018 , Pages 266-275 ; 01448617 (ISSN) ; Alemzadeh, I ; Vossoughi, M ; Chackoshian Khorasani, A ; Sharif University of Technology
Abstract
The aim of this study was to investigate the co-microencapsulation of Lactobacillus plantarum and DHA-rich oil in a novel gastrointestinal-resistant biocomposite composed of alginate, pectin and gelatin. The optimal biocomposite consisted of 1.06% alginate, 0.55% pectin and 0.39% gelatin showed 88.66% survivability of the microencapsulated cells compared to the free cells (50.36%). In addition, co-microencapsule containing probiotic and DHA fatty acid was synthesized and physicochemically analyzed using SEM, FTIR, TGA, XRD. The results from SEM clearly confirmed that cells were completely entrapped in the matrix and DHA increased smoothness and compactness of the surface of the particles....
Conductive nanofiber scaffold for bone tissue engineering
, Article 24th Iranian Conference on Biomedical Engineering and 2017 2nd International Iranian Conference on Biomedical Engineering, ICBME 2017, 30 November 2017 through 1 December 2017 ; 2018 ; 9781538636091 (ISBN) ; Mashayekhan, S ; Abbaszadeh, H. A ; Ansarizadeh, M ; Sharif University of Technology
Institute of Electrical and Electronics Engineers Inc
2018
Abstract
In order to fabricate nanofiber scaffold for bone tissue engineering, electrospinning technique was employed. This technique produces nanofiberous scaffold supporting cell adhesion, migration, and proliferation. Here, we developed a novel conductive scaffold from poly-caprolactone, gelatin, and poly aniline/graphene nanoparticles. In this study, co-electrospinning was utilized to fabricate composite electrospun scaffold. The effect of polyaniline/graphene (PAG) nanoparticles on the mechanical properties and electrical conductivity of this hybrid scaffold was investigated. The result showed that PAG nanoparticles enbance both mechanical properties and electrical conductivity of the scaffolds....
Drug delivery systems and materials for wound healing applications
, Article Advanced Drug Delivery Reviews ; Volume 127 , 2018 , Pages 138-166 ; 0169409X (ISSN) ; Rinoldi, C ; Schot, M ; Saheb Kashaf, S ; Sharifi, F ; Jalilian, E ; Nuutila, K ; Giatsidis, G ; Mostafalu, P ; Derakhshandeh, H ; Yue, K ; Swieszkowski, W ; Memic, A ; Tamayol, A ; Khademhosseini, A ; Sharif University of Technology
Elsevier B.V
2018
Abstract
Chronic, non-healing wounds place a significant burden on patients and healthcare systems, resulting in impaired mobility, limb amputation, or even death. Chronic wounds result from a disruption in the highly orchestrated cascade of events involved in wound closure. Significant advances in our understanding of the pathophysiology of chronic wounds have resulted in the development of drugs designed to target different aspects of the impaired processes. However, the hostility of the wound environment rich in degradative enzymes and its elevated pH, combined with differences in the time scales of different physiological processes involved in tissue regeneration require the use of effective drug...
Microstructure and characteristic properties of gelatin/chitosan scaffold prepared by the freeze-gelation method
, Article Materials Research Express ; Volume 6, Issue 11 , 2019 ; 20531591 (ISSN) ; Kamali, A ; Bahrani Fard, M. R ; Sharif University of Technology
Institute of Physics Publishing
2019
Abstract
Three-dimensional porous scaffolds are essential in tissue engineering applications. One of the most conventional methods to form porosity in scaffolds is freeze-drying, which is not energy efficient and cost effective. Therefore in this work, it was experimentally investigated whether gelatin, with its unique mechanical properties and cell binding applications, could be used as a comprising polymer of scaffolds with porous structure made by the freeze-gelation method. Chitosan, gelatin and chitosan/gelatin scaffolds were fabricated by the freeze-gelation method and their behaviors, determined by analysis of scanning electron microscopy images, Fourier transform infrared spectroscopy,...
A hydrogen-bonded extracellular matrix-mimicking bactericidal hydrogel with radical scavenging and hemostatic function for ph-responsive wound healing acceleration
, Article Advanced Healthcare Materials ; 2020 ; Correia, A ; Hasany, M ; Figueiredo, P ; Dobakhti, F ; Eskandari, M. R ; Hosseini, H ; Abiri, R ; Khorshid, S ; Hirvonen, J ; Santos, H. A ; Shahbazi, M. A ; Sharif University of Technology
Wiley-VCH Verlag
2020
Abstract
Generation of reactive oxygen species, delayed blood clotting, prolonged inflammation, bacterial infection, and slow cell proliferation are the main challenges of effective wound repair. Herein, a multifunctional extracellular matrix-mimicking hydrogel is fabricated through abundant hydrogen bonding among the functional groups of gelatin and tannic acid (TA) as a green chemistry approach. The hydrogel shows adjustable physicochemical properties by altering the concentration of TA and it represents high safety features both in vitro and in vivo on fibroblasts, red blood cells, and mice organs. In addition to the merit of facile encapsulation of cell proliferation-inducing hydrophilic drugs,...
A porous hydrogel-electrospun composite scaffold made of oxidized alginate/gelatin/silk fibroin for tissue engineering application
, Article Carbohydrate Polymers ; Volume 245 , 2020 ; Alemzadeh, I ; Vossoughi, M ; Sharif University of Technology
Elsevier Ltd
2020
Abstract
In the article, a bilayer nanocomposite scaffold made of oxidized alginate (OAL), gelatin (G), and silk fibroin (SF) has been prepared via combining electrospinning, in situ gas foaming, in situ crosslinking and freeze drying methods. The physicochemical and mechanical properties, as well as thermal stability of the proposed composite, have been investigated by SEM, FTIR, XRD, tensile, and TGA analysis. The data indicate that structure and degree of crosslinking play a vital role in adjusting the physical and mechanical properties of composite scaffolds. Further, the authors find a favorable adipose-derived mesenchymal stem cell's (AMSC) attachment and distribution within this novel...
In-situ crosslinking of electrospun gelatin-carbodiimide nanofibers: fabrication, characterization, and modeling of solution parameters
, Article Chemical Engineering Communications ; 2020 ; Alemzadeh, I ; Vossoughi, M ; Shamloo, A ; Sharif University of Technology
Taylor and Francis Ltd
2020
Abstract
This work has focused on in-situ crosslinking of gelatin (G) to produce electrospun scaffold with improved fiber morphology retention and mechanical properties. As per this approach, we prepared G nanofibers through mixing G, 1-ethyl-3-(3 dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) in the new solvent system. Response surface methodology (RSM) was employed to study the influence of solution parameters on fiber diameter. The morphological structure was examined, and the appropriate level of setting to obtain smooth fibers with a favorable diameter was reported. Results revealed using EDC/NHS for in-situ crosslinking improves the mechanical properties...
Biodegradable nanopolymers in cardiac tissue engineering: from concept towards nanomedicine
, Article International Journal of Nanomedicine ; Volume 15 , 2020 , Pages 4205-4224 ; Rabiee, N ; Hajebi, S ; Ahmadi, S ; Fatahi, Y ; Hosseini, M ; Bagherzadeh, M ; Ghadiri, A. M ; Rabiee, M ; Jajarmi, V ; Webster, T. J ; Sharif University of Technology
Dove Medical Press Ltd
2020
Abstract
Cardiovascular diseases are the number one cause of heart failure and death in the world, and the transplantation of the heart is an effective and viable choice for treatment despite presenting many disadvantages (most notably, transplant heart availability). To overcome this problem, cardiac tissue engineering is considered a promising approach by using implantable artificial blood vessels, injectable gels, and cardiac patches (to name a few) made from biodegradable polymers. Biodegradable polymers are classified into two main categories: natural and synthetic polymers. Natural biodegradable polymers have some distinct advantages such as biodegradability, abundant availability, and...
Injectable hydrogels based on oxidized alginate-gelatin reinforced by carbon nitride quantum dots for tissue engineering
, Article International Journal of Pharmaceutics ; Volume 602 , 2021 ; 03785173 (ISSN) ; Salavati Niasari, M ; Mohandes, F ; Sharif University of Technology
Elsevier B.V
2021
Abstract
Stem cell treatment is promising in the various disorders treatment, but its effect is confined by the adverse conditions in the damaged tissues. The utilization of hydrogels has been suggested as a procedure to defeat this issue by developing the engraftment and survival of injected stem cells. Specifically, injectable hydrogels have drawn much attention due to their shape adaptability, ease of use, and the capability to reach body parts that are hard to access. In this study, the thermosensitive injectable hydrogels based on oxidized alginate, gelatin, and carbon nitride quantum dots (CNQDs) have been fabricated for tissue engineering. The mechanical characteristics of the nanocomposite...
Fabrication and evaluation of chitosan/gelatin/PVA hydrogel incorporating honey for wound healing applications: An in vitro, in vivo study
, Article International Journal of Pharmaceutics ; Volume 592 , 2021 ; 03785173 (ISSN) ; Aghababaie, Z ; Afjoul, H ; Jami, M ; Bidgoli, M. R ; Vossoughi, M ; Ramazani, A ; Kamyabhesari, K ; Sharif University of Technology
Elsevier B.V
2021
Abstract
In this study, physically cross-linked hydrogels were developed by freezing-thawing method while different concentrations of honey were included into the hydrogels for accelerated wound healing. The hydrogel was composed of chitosan, polyvinyl alcohol (PVA), and gelatin with the ratio of 2:1:1 (v/v), respectively. Further, the effect of honey concentrations on antibacterial properties, and cell behavior was investigated. In vivo studies, including wound healing mechanism using rat model and histological analysis of section tissue samples were performed. The results illustrated that the incorporation of honey in hydrogels increased the ultimate strain of hydrogels approximately two times,...
Fabrication of a novel 3D scaffold for cartilage tissue repair: In-vitro and in-vivo study
, Article Materials Science and Engineering C ; Volume 128 , 2021 ; 09284931 (ISSN) ; Shamloo, A ; Sharif University of Technology
Elsevier Ltd
2021
Abstract
Self-repairing is not an advanced ability of articular cartilage. Tissue engineering has provided a novel way for reconstructing cartilage using natural polymers because of their biocompatibility and bio-functionality. The purpose of cartilage tissue engineering is to design a scaffold with proper pore structure and similar biological and mechanical properties to the native tissue. In this study, porous scaffolds prepared from gelatin, chitosan and silk fibroin were blended with varying ratios. Between the blends of chitosan (C), gelatin (G) and silk fibroin (S), the scaffold with the weight per volume ratio of 2:2:3 (w/v) showed the most favorable and higher certain properties than the...
Immunomodulating hydrogels as stealth platform for drug delivery applications
, Article Pharmaceutics ; Volume 14, Issue 10 , 2022 ; 19994923 (ISSN) ; Yilmaz Aykut, D ; Tourk, F. M ; Bassous, N ; Barroso Zuppa, M ; Shawl, A. I ; Ashraf, S. S ; Avci, H ; Hassan, S ; Sharif University of Technology
MDPI
2022
Abstract
Non-targeted persistent immune activation or suppression by different drug delivery platforms can cause adverse and chronic physiological effects including cancer and arthritis. Therefore, non-toxic materials that do not trigger an immunogenic response during delivery are crucial for safe and effective in vivo treatment. Hydrogels are excellent candidates that can be engineered to control immune responses by modulating biomolecule release/adsorption, improving regeneration of lymphoid tissues, and enhancing function during antigen presentation. This review discusses the aspects of hydrogel-based systems used as drug delivery platforms for various diseases. A detailed investigation on...
Cell-Seeded biomaterial scaffolds: the urgent need for unanswered accelerated angiogenesis
, Article International Journal of Nanomedicine ; Volume 17 , 2022 , Pages 1035-1068 ; 11769114 (ISSN) ; Shokrani, A ; Sajadi, S. M ; Seidi, F ; Mashhadzadeh, A. H ; Rabiee, N ; Saeb, M. R ; Aminabhavi, T ; Webster, T. J ; Sharif University of Technology
Dove Medical Press Ltd
2022
Abstract
One of the most arduous challenges in tissue engineering is neovascularization, without which there is a lack of nutrients delivered to a target tissue. Angiogenesis should be completed at an optimal density and within an appropriate period of time to prevent cell necrosis. Failure to meet this challenge brings about poor functionality for the tissue in comparison with the native tissue, extensively reducing cell viability. Prior studies devoted to angiogenesis have provided researchers with some biomaterial scaffolds and cell choices for angiogenesis. For example, while most current angiogenesis approaches require a variety of stimulatory factors ranging from biomechanical to biomolecular...