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lab-on-a-chip
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Urine Analysis by a Microfluidic Paper-Based Device for Renal Function Test
, M.Sc. Thesis Sharif University of Technology ; Saadatmand, Maryam (Supervisor)
Abstract
Chronic kidney disease is one of the silent diseases that progresses slowly, often without symptoms, and can eventually lead to kidney loss. Early detection of this disease can help diagnose it and prevent further problems. Urinary creatinine is an effective biomarker for early diagnosis of chronic kidney disease, but methods available in clinics are problematic for people living in disadvantaged areas because of the high cost and the need for 24-hour urine collection. Currently, paper-based microfluidic devices are being developed because they are easy to manufacture, inexpensive, the fluid is permeable by capillary force, and no additional equipment is needed. In this work, to fabricate a...
Temperature rise in electroosmotic flow of typical non-newtonian biofluids through rectangular microchannels
, Article Journal of Heat Transfer ; Volume 136, Issue 3 , March , 2014 ; ISSN: 00221481 ; Sadeghi, A ; Saidi, M. H ; Chakraborty, S ; Sharif University of Technology
Abstract
Electroosmosis is the main mechanism for flow generation in lab-on-a-chip (LOC) devices. The temperature rise due to the Joule heating phenomenon, associated with the electroosmosis, may be detrimental for samples being considered in LOCs. Hence, a complete understanding of the heat transfer physics associated with the electroosmotic flow is of high importance in design and active control of LOCs. The objective of the present study is to estimate the temperature rise and the thermal entry length in electroosmotic flow through rectangular microchannels, having potential applications in LOC devices. Along this line, the power-law rheological model is used to account for non-Newtonian behavior...
Reduction of production rate in y-shaped microreactors in the presence of viscoelasticity
, Article Analytica Chimica Acta ; Volume 990 , 2017 , Pages 121-134 ; 00032670 (ISSN) ; Saidi, M. H ; Sadeghi, A ; Sharif University of Technology
Abstract
The viscoelasticity effects on the reaction-diffusion rates in a Y-shaped microreactor are studied utilizing the PTT rheological model. The flow is assumed to be fully developed and considered to be created under a combined action of electroosmotic and pressure forces. In general, finite-volume-based numerical simulations are conducted to handle the problem; however, analytical solutions based on the depthwise averaging approach are also obtained for the case for which there is no reaction between the inlet components. The analytical solutions are found to predict accurate results when the width to height ratio is at least 10 and acceptable results for lower aspect ratios. An investigation...
Recent approaches to mRNA vaccine delivery by lipid-based vectors prepared by continuous-flow microfluidic devices
, Article Future medicinal chemistry ; Volume 14, Issue 21 , 2022 , Pages 1561-1581 ; 17568927 (ISSN) ; Tavakoli, A ; Mohammadghasemi, H ; Karimi, A ; Ai, J ; Rabiee, M ; Rabiee, N ; Sharif University of Technology
NLM (Medline)
2022
Abstract
Advancements in nanotechnology have resulted in the introduction of several nonviral delivery vectors for the nontoxic, efficient delivery of encapsulated mRNA-based vaccines. Lipid- and polymer-based nanoparticles (NP) have proven to be the most potent delivery systems, providing increased delivery efficiency and protection of mRNA molecules from degradation. Here, the authors provide an overview of the recent studies carried out using lipid NPs and their functionalized forms, polymeric and lipid-polymer hybrid nanocarriers utilized mainly for the encapsulation of mRNAs for gene and immune therapeutic applications. A microfluidic system as a prevalent methodology for the preparation of NPs...
Polyamide/titania hollow nanofibers prepared by core–shell electrospinning as a microextractive phase in a fabricated sandwiched format microfluidic device
, Article Journal of Chromatography A ; Volume 1528 , 2017 , Pages 1-9 ; 00219673 (ISSN) ; Hashemi Hedeshi, M ; Bagheri, H ; Sharif University of Technology
Abstract
In this study, a low–cost microfluidic device from polymethyl methacrylate was fabricated by laser engraving technique. The device is consisted of a central chip unit with an aligned microchannel. Both sides of the engraved microchannel were sandwiched by two synthesized sheets from polyamide/titania (PA/TiO2) hollow nanofibers as extractive phases. The inlet and outlet of the device were connected to the polyether ether ketone tubes, while a peristaltic pump was used to deliver both sample and desorbing solvent through the microchannel. The recorded scanning electron microscopy images from the surface of the synthesized PA/TiO2 nanofibers, exhibit a good degree of homogeneity and porosity...
Newtonian and generalized Newtonian reacting flows in serpentine microchannels: pressure driven and centrifugal microfluidics
, Article Journal of Non-Newtonian Fluid Mechanics ; Volume 251 , January , 2018 , Pages 88-96 ; 03770257 (ISSN) ; Shamloo, A ; Sharif University of Technology
Elsevier B.V
2018
Abstract
This paper presents a comprehensive 3D numerical simulation of reacting flows in micro scale dimension through centrifugal, or Lab-On-a-CD (LOCD), and pressure-driven, or Lab-On-a-Chip (LOC) devices. Three different serpentine channel configurations (rectangular, triangular and sinusoidal) are investigated. In these configurations, two chemical species enter from two inlets and according to an irreversible chemical reaction, start yielding other species. Both Newtonian and generalized Newtonian fluids are considered in the simulations and the results are compared for both LOC and LOCD devices. Besides, the effects of different parameters such as the aspect ratio of channels’ cross section,...
Nanotechnology-assisted microfluidic systems: From bench to bedside
, Article Nanomedicine ; Volume 16, Issue 3 , 2021 , Pages 237-258 ; 17435889 (ISSN) ; Ahmadi, S ; Fatahi, Y ; Rabiee, M ; Bagherzadeh, M ; Dinarvand, R ; Bagheri, B ; Zarrintaj, P ; Saeb, M. R ; Webster, T. J ; Sharif University of Technology
Future Medicine Ltd
2021
Abstract
With significant advancements in research technologies, and an increasing global population, microfluidic and nanofluidic systems (such as point-of-care, lab-on-a-chip, organ-on-a-chip, etc) have started to revolutionize medicine. Devices that combine micron and nanotechnologies have increased sensitivity, precision and versatility for numerous medical applications. However, while there has been extensive research on microfluidic and nanofluidic systems, very few have experienced wide-spread commercialization which is puzzling and deserves our collective attention. For the above reasons, in this article, we review research advances that combine micro and nanotechnologies to create the next...
Modeling, simulation, and employing dilution–dialysis microfluidic chip (DDMC) for heightening proteins refolding efficiency
, Article Bioprocess and Biosystems Engineering ; Volume 41, Issue 5 , 2018 , Pages 707-714 ; 16157591 (ISSN) ; Masoudi, M. M ; Shamloo, A ; Habibi Rezaei, M ; Moosavi Movahedi, A. A ; Sharif University of Technology
Springer Verlag
2018
Abstract
Miniaturized systems based on the principles of microfluidics are widely used in various fields, such as biochemical and biomedical applications. Systematic design processes are demanded the proper use of these microfluidic devices based on mathematical simulations. Aggregated proteins (e.g., inclusion bodies) in solution with chaotropic agents (such as urea) at high concentration in combination with reducing agents are denatured. Refolding methods to achieve the native proteins from inclusion bodies of recombinant protein relying on denaturant dilution or dialysis approaches for suppressing protein aggregation is very important in the industrial field. In this paper, a modeling approach is...
Microfluidic devices with gold thin film channels for chemical and biomedical applications: a review
, Article Biomedical Microdevices ; Volume 21, Issue 4 , 2019 ; 13872176 (ISSN) ; Rabiee, N ; Rabiee, M ; Bagherzadeh, M ; Tahriri, M ; Tayebi, L ; Hamblin, M. R ; Sharif University of Technology
Springer New York LLC
2019
Abstract
Microfluidic systems (MFS) provide a range of advantages in biomedical applications, including improved controllability of material characteristics and lower consumption of reagents, energy, time and money. Fabrication of MFS employs various materials, such as glass, silicon, ceramics, paper, and metals such as gold, copper, aluminum, chromium and titanium. In this review, gold thin film microfluidic channels (GTFMFC) are discussed with reference to fabrication methods and their diverse use in chemical and biomedical applications. The advantages of gold thin films (GTF) include flexibility, ease of manufacture, adhesion to polymer surfaces, chemical stability, good electrical conductivity,...
Microfluidic-based approaches in targeted cell/particle separation based on physical properties: fundamentals and applications
, Article Small ; Volume 16, Issue 29 , 2020 ; Shamloo, A ; Ahadian, S ; Amirifar, L ; Akbari, J ; Goudie, M. J ; Lee, K ; Ashammakhi, N ; Dokmeci, M. R ; Di Carlo, D ; Khademhosseini, A ; Sharif University of Technology
Wiley-VCH Verlag
2020
Abstract
Cell separation is a key step in many biomedical research areas including biotechnology, cancer research, regenerative medicine, and drug discovery. While conventional cell sorting approaches have led to high-efficiency sorting by exploiting the cell's specific properties, microfluidics has shown great promise in cell separation by exploiting different physical principles and using different properties of the cells. In particular, label-free cell separation techniques are highly recommended to minimize cell damage and avoid costly and labor-intensive steps of labeling molecular signatures of cells. In general, microfluidic-based cell sorting approaches can separate cells using “intrinsic”...
Label-free electrochemical microfluidic biosensors: futuristic point-of-care analytical devices for monitoring diseases
, Article Microchimica Acta ; Volume 189, Issue 7 , 2022 ; 00263672 (ISSN) ; Samadi Pakchin, P ; Shamloo, A ; Mota, A ; de la Guardia, M ; Omidian, H ; Omidi, Y ; Sharif University of Technology
Springer
2022
Abstract
The integration of microfluidics with electrochemical analysis has resulted in the development of single miniaturized detection systems, which allows the precise control of sample volume with multianalyte detection capability in a cost- and time-effective manner. Microfluidic electrochemical sensing devices (MESDs) can potentially serve as precise sensing and monitoring systems for the detection of molecular markers in various detrimental diseases. MESDs offer several advantages, including (i) automated sample preparation and detection, (ii) low sample and reagent requirement, (iii) detection of multianalyte in a single run, (iv) multiplex analysis in a single integrated device, and (v)...
Engineered Biomimetic Membranes for Organ-on-a-Chip
, Article ACS Biomaterials Science and Engineering ; Volume 8, Issue 12 , 2022 , Pages 5038-5059 ; 23739878 (ISSN) ; Rasouli, F ; Jahangiri, S ; Ahmadi, S ; Rabiee, N ; Ramezani Farani, M ; Akhavan, O ; Asadnia, M ; Fatahi, Y ; Hong, S ; Lee, J ; Lee, J ; Hahn, S. K ; Sharif University of Technology
American Chemical Society
2022
Abstract
Organ-on-a-chip (OOC) systems are engineered nanobiosystems to mimic the physiochemical environment of a specific organ in the body. Among various components of OOC systems, biomimetic membranes have been regarded as one of the most important key components to develop controllable biomimetic bioanalysis systems. Here, we review the preparation and characterization of biomimetic membranes in comparison with the features of the extracellular matrix. After that, we review and discuss the latest applications of engineered biomimetic membranes to fabricate various organs on a chip, such as liver, kidney, intestine, lung, skin, heart, vasculature and blood vessels, brain, and multiorgans with...
Emerging phospholipid nanobiomaterials for biomedical applications to lab-on-a-chip, drug delivery, and cellular engineering
, Article ACS Applied Bio Materials ; 2021 ; 25766422 (ISSN) ; Rabiee, N ; Ahmadi, S ; Jahangiri, S ; Sajadi, S. M ; Akhavan, O ; Saeb, M. R ; Kwon, W ; Kim, M ; Hahn, S. K ; Sharif University of Technology
American Chemical Society
2021
Abstract
The design of advanced nanobiomaterials to improve analytical accuracy and therapeutic efficacy has become an important prerequisite for the development of innovative nanomedicines. Recently, phospholipid nanobiomaterials including 2-methacryloyloxyethyl phosphorylcholine (MPC) have attracted great attention with remarkable characteristics such as resistance to nonspecific protein adsorption and cell adhesion for various biomedical applications. Despite many recent reports, there is a lack of comprehensive review on the phospholipid nanobiomaterials from synthesis to diagnostic and therapeutic applications. Here, we review the synthesis and characterization of phospholipid nanobiomaterials...
Design and simulation of a microfluidic device for acoustic cell separation
, Article Ultrasonics ; Volume 84 , March , 2018 , Pages 234-243 ; 0041624X (ISSN) ; Boodaghi, M ; Sharif University of Technology
Elsevier B.V
2018
Abstract
Experimental acoustic cell separation methods have been widely used to perform separation for different types of blood cells. However, numerical simulation of acoustic cell separation has not gained enough attention and needs further investigation since by using numerical methods, it is possible to optimize different parameters involved in the design of an acoustic device and calculate particle trajectories in a simple and low cost manner before spending time and effort for fabricating these devices. In this study, we present a comprehensive finite element-based simulation of acoustic separation of platelets, red blood cells and white blood cells, using standing surface acoustic waves...
Design and fabrication of a centrifugal microfluidic disc including septum valve for measuring hemoglobin A1c in human whole blood using immunoturbidimetry method
, Article Talanta ; Volume 190 , 2018 , Pages 134-139 ; 00399140 (ISSN) ; Saadatmand, M ; Bakhtiari, M. R ; Eghbal, M ; Sharif University of Technology
Elsevier B.V
2018
Abstract
Diabetes mellitus is a global endemic with a rapidly increasing prevalence in both developing and developed countries. Recently, hemoglobin A1c has been recommended by the American Diabetes Associations as a possible substitute for fasting blood glucose for the diagnosis of diabetes, because it is an indicator of long-term glycemic control. Also, centrifugal microfluidic systems have good potential for use in the point of care testing systems. In this study, a centrifugal microfluidic disc was designed and manufactured to measure hemoglobin A1c in whole blood using an immunoturbidimetry based method. Also, a new passive valve, named septum valve, was presented to precisely control the entry...
Deriving an analytical model for hydro-magnetic micro flow controller
, Article 6th International Conference on Nanochannels, Microchannels, and Minichannels, ICNMM2008, Darmstadt, 23 June 2008 through 25 June 2008 ; Issue PART B , June , 2008 , Pages 1139-1146 ; 0791848345 (ISBN); 9780791848340 (ISBN) ; Shafii, M. B ; ASME ; Sharif University of Technology
2008
Abstract
Fluid control, namely pumping and valving, is a critical factor in the performance of micro-fluidic systems. In recent years a variety of micro-fluidic systems are developed for the purpose of miniaturizing fluid handling, and chemical analysis to develop Lab On a Chip (LOC) technology. The mentioned facts resulted in design and fabrication of a novel hydromagnetic flow controller. The idea behind this device is that magnetic particles, mixed and dispersed in a carrier liquid, can be accumulated in the form of a piston. Depending upon dragging speed of these pistons, which itself is a function of switching time, this device can be used to either increase (pumping) or decrease (valving) the...
Challenge in particle delivery to cells in a microfluidic device
, Article Drug Delivery and Translational Research ; Volume 8, Issue 3 , 2018 , Pages 830-842 ; 2190393X (ISSN) ; Saidi, M. S ; Kashaninejad, N ; Nguyen, N. T ; Sharif University of Technology
Springer Verlag
2018
Abstract
Micro and nanotechnology can potentially revolutionize drug delivery systems. Novel microfluidic systems have been employed for the cell culture applications and drug delivery by micro and nanocarriers. Cells in the microchannels are under static and dynamic flow perfusion of culture media that provides nutrition and removes waste from the cells. This exerts hydrostatic and hydrodynamic forces on the cells. These forces can considerably affect the functions of the living cells. In this paper, we simulated the flow of air, culture medium, and the particle transport and deposition in the microchannels under different angles of connection inlet. It was found that the shear stress induced by the...
Carbon nanotubes in microfluidic lab-on-a-chip technology: current trends and future perspectives
, Article Microfluidics and Nanofluidics ; Volume 21, Issue 9 , 2017 ; 16134982 (ISSN) ; Amiri, H ; Zare, H ; Masroor, M ; Hasanzadeh, A ; Beyzavi, A ; Aref, A. R ; Karimi, M ; Hamblin, M. R ; Sharif University of Technology
Abstract
Advanced nanomaterials such as carbon nanotubes (CNTs) display unprecedented properties such as strength, electrical conductance, thermal stability, and intriguing optical properties. These properties of CNT allow construction of small microfluidic devices leading to miniaturization of analyses previously conducted on a laboratory bench. With dimensions of only millimeters to a few square centimeters, these devices are called lab-on-a-chip (LOC). A LOC device requires a multidisciplinary contribution from different fields and offers automation, portability, and high-throughput screening along with a significant reduction in reagent consumption. Today, CNT can play a vital role in many parts...
Cancer cell enrichment on a centrifugal microfluidic platform using hydrodynamic and magnetophoretic techniques
, Article Scientific Reports ; Volume 11, Issue 1 , 2021 ; 20452322 (ISSN) ; Naghdloo, A ; Besanjideh, M ; Sharif University of Technology
Nature Research
2021
Abstract
Isolation of rare cancer cells is one of the important and valuable stages of cancer research. Regarding the rarity of cancer cells in blood samples, it is important to invent an efficient separation device for cell enrichment. In this study, two centrifugal microfluidic devices were designed and fabricated for the isolation of rare cancer cells. The first design (passive plan) employs a contraction–expansion array (CEA) microchannel which is connected to a bifurcation region. This device is able to isolate the target cells through inertial effects and bifurcation law. The second design (hybrid plan) also utilizes a CEA microchannel, but instead of using the bifurcation region, it is...
Brain-on-a-chip: Recent advances in design and techniques for microfluidic models of the brain in health and disease
, Article Biomaterials ; Volume 285 , 2022 ; 01429612 (ISSN) ; Shamloo, A ; Nasiri, R ; de Barros, N. R ; Wang, Z. Z ; Unluturk, B. D ; Libanori, A ; Ievglevskyi, O ; Diltemiz, S. E ; Sances, S ; Balasingham, I ; Seidlits, S. K ; Ashammakhi, N ; Sharif University of Technology
Elsevier Ltd
2022
Abstract
Recent advances in biomaterials, microfabrication, microfluidics, and cell biology have led to the development of organ-on-a-chip devices that can reproduce key functions of various organs. Such platforms promise to provide novel insights into various physiological events, including mechanisms of disease, and evaluate the effects of external interventions, such as drug administration. The neuroscience field is expected to benefit greatly from these innovative tools. Conventional ex vivo studies of the nervous system have been limited by the inability of cell culture to adequately mimic in vivo physiology. While animal models can be used, their relevance to human physiology is uncertain and...