Search for: metabolism
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    Metabolic profiling of seminal plasma from teratozoospermia patients

    , Article Journal of Pharmaceutical and Biomedical Analysis ; Volume 178 , January , 2020 Mehrparvar, B ; Chashmniam, S ; Nobakht, F ; Amini, M ; Javidi, A ; Minai Tehrani, A ; Arjmand, B ; Gilany, K ; Sharif University of Technology
    Elsevier B. V  2020
    Teratozoospermia is one of conditions that can cause male infertility. The mechanism of teratozoospermia remains unclear. The knowledge of the metabolites in human seminal plasma (HSP) is meaningful for the pathological study of teratozoospermia. Analysis of changed metabolites in HSP can help understand the cellular mechanism, find the novel biomarkers and subsequently design a diagnosis test. In this study, the analysis of samples performed by proton nuclear magnetic resonance spectroscopy (1H NMR spectroscopy) to identify the various metabolites, with the aim of finding metabolic profiles and biomarkers related to male infertility. Eighteen de-regulated metabolites were identified in... 

    Synthesis, crystal structure and catalytic activity of a novel Mo(VI)-oxazoline complex in highly efficient oxidation of sulfides to sulfoxides by urea hydrogen peroxide

    , Article Inorganica Chimica Acta ; Volume 361, Issue 7 , 2008 , Pages 2019-2024 ; 00201693 (ISSN) Bagherzadeh, M ; Tahsini, L ; Latifi, R ; Ellern, A ; Woo, L. K ; Sharif University of Technology
    Elsevier S.A  2008
    Novel molybdenum complex, cis-[MoO2(phox)2] has been synthesized and characterized by IR, 1H NMR, elemental analyses (CHN), and X-ray molecular structure determination methods. This complex was found to be an efficient, selective catalyst for the oxidation of various sulfides to sulfoxides with urea hydrogen peroxide (UHP) in excellent yields (100% for diallylsulfide) and short reaction times (20 min) at room temperature. The catalytic system oxidizes diallylsulfide chemoselectively to its corresponding sulfoxide without any over oxidation in double bond. © 2007 Elsevier B.V. All rights reserved  

    Molecular simulation of protein dynamics in nanopores. I. Stability and folding

    , Article Journal of Chemical Physics ; Volume 128, Issue 11 , 2008 ; 00219606 (ISSN) Javidpour, L ; Rahimi Tabar, M. R ; Sahimi, M ; Sharif University of Technology
    Discontinuous molecular dynamics simulations, together with the protein intermediate resolution model, an intermediate-resolution model of proteins, are used to carry out several microsecond-long simulations and study folding transition and stability of α -de novo-designed proteins in slit nanopores. Both attractive and repulsive interaction potentials between the proteins and the pore walls are considered. Near the folding temperature Tf and in the presence of the attractive potential, the proteins undergo a repeating sequence of folding/partially folding/unfolding transitions, with Tf decreasing with decreasing pore sizes. The unfolded states may even be completely adsorbed on the pore's... 

    N-benzyl DABCO tribromide - Promoted oxidative coupling of benzyl cyanides: A convenient procedure for the synthesis of α, α′- dicyanostilbenes

    , Article Synthetic Communications ; Volume 38, Issue 11 , 2008 , Pages 1694-1702 ; 00397911 (ISSN) Matloubi Moghaddam, F ; Zargarani, D ; Boeini, H. Z ; Sharif University of Technology
    A convenient and efficient procedure was developed for preparing α,α′-icyanostilbenes through the oxidative coupling reaction of benzyl cyanide derivatives using N-benzyl DABCO tribromide as the oxidative bromination reagent in the presence of K2CO3 as a base. Copyright © Taylor & Francis Group, LLC  

    Hydrocarbon degradation by thermophilic Nocardia otitidiscaviarum strain TSH1: Physiological aspects

    , Article Journal of Basic Microbiology ; Volume 47, Issue 6 , December , 2007 , Pages 534-539 ; 0233111X (ISSN) Zeinali, M ; Vossoughi, M ; Ardestani, S. K ; Babanezhad, E ; Masoumian, M ; Sharif University of Technology
    Indigenous thermophilic hydrocarbon degraders are of special significance for the bioremediation of oil-contaminated desert soils with ambient temperature of 45-50°C. The first objective of this study was to demonstrate the hydrocarbon-degrading capability of Nocardia otitidiscaviarum TSH1 (DSM 45036) which grows optimally at 50°C. Analysis of the metabolic profile of the strain TSH1 showed that it could metabolize phenol, intermediate-chain-length n-alkanes and some polycyclic aromatic hydrocarbons (PAHs) ranging in size from two to four fused rings efficiently, but not toluene and xylene. N. otitidiscaviarum TSH1 was able to survive and grow at phenol concentrations up to 875 mg l-1. For... 

    Preparation of Ag-Al₂O₃ nano structures by combustion method and investigation of photocatalytic activity

    , Article International Journal of Applied Ceramic Technology ; Volume 18, Issue 6 , 2021 , Pages 2064-2074 ; 1546542X (ISSN) Einafshar, E ; Khodadadipoor, Z ; Fazli, M ; Einafshar, N ; Mohebbi Zinab, J ; Asaei, S ; Sharif University of Technology
    John Wiley and Sons Inc  2021
    In this research, Ag-Al2O3 nanostructures have been prepared via combustion synthesis and ammonium acetate and urea have been applied as fuels. The prepared Ag-Al2O3 nanostructures were characterized by DTA, XRD, SEM, TEM, and BET spectroscopy. The effect of different ratios of silver to alumina and fuel percentage on morphology and particle size of prepared products were investigated. The results showed that using ammonium acetate fuel led to the production of Ag-γ-Al2O3 nanocompounds, while using urea produced Ag-α-Al2O3. Also, the photocatalytic activity of Ag-Al2O3 nanostructures for Congo red degradation was evaluated by UV-Vis diffuse reflectance spectroscopy. The photocatalytic... 

    Downregulation of ITM2A gene expression in macrophages of patients with ankylosing spondylitis

    , Article International Archives of Allergy and Immunology ; Volume 182, Issue 11 , 2021 , Pages 1113-1121 ; 10182438 (ISSN) Lari, A ; Pourbadie, H. G ; Jafari, M ; Sharifi Zarchi, A ; Akhtari, M ; Nejatbakhsh Samimi, L ; Jamshidi, A ; Mahmoudi, M ; Sharif University of Technology
    S. Karger AG  2021
    Objectives: Ankylosing spondylitis (AS) is a rheumatic disorder that is mostly determined by genetic and environmental factors. Given the known importance of macrophage in AS pathogenesis, we investigated the transcriptional profile of macrophage cells in the disease. Methods and Results: Two approaches of differential expression and subsequently, weighted gene co-expression network analysis was utilized to analyze a publicly available microarray dataset of macrophages. Integral membrane protein 2A (ITM2A) was among the most significant genes with a decreased trend in the common results of both methods. In order to confirm the finding, the expression of ITM2A was evaluated in... 

    Effect of various carbon sources on biomass and lipid production of Chlorella vulgaris during nutrient sufficient and nitrogen starvation conditions

    , Article Bioresource Technology ; Volume 180 , 2015 , Pages 311-317 ; 09608524 (ISSN) Abedini Najafabadi, H ; Malekzadeh, M ; Jalilian, F ; Vossoughi, M ; Pazuki, G ; Sharif University of Technology
    In this research, a two-stage process consisting of cultivation in nutrient rich and nitrogen starvation conditions was employed to enhance lipid production in Chlorella vulgaris algal biomass. The effect of supplying different organic and inorganic carbon sources on cultivation behavior was investigated. During nutrient sufficient condition (stage I), the highest biomass productivity of 0.158. ±. 0.011. g/L/d was achieved by using sodium bicarbonate followed by 0.130. ±. 0.013, 0.111. ±. 0.005 and 0.098. ±. 0.003. g/L/d for sodium acetate, carbon dioxide and molasses, respectively. Cultivation under nitrogen starvation process (stage II) indicated that the lipid and fatty acid content... 

    The metabolomics signature associated with responsiveness to steroid therapy in focal segmental glomerulosclerosis: A pilot study

    , Article Revista de Investigacion Clinica ; Volume 71, Issue 2 , 2019 , Pages 106-115 ; 00348376 (ISSN) Chashmniam, S ; Kalantari, S ; Nafar, M ; Boroumandnia, N ; Sharif University of Technology
    Instituto Nacional de la Nutricion Salvador Zubiran  2019
    Background: Focal segmental glomerulosclerosis (FSGS) is considered one of the most severe glomerular diseases and around 80% of cases are resistant to steroid treatment. Since a large proportion of steroid-resistant (SR) FSGS patients progress to end-stage renal disease, other therapeutic strategies may benefit this population. However, identification of non-invasive biomarkers to predict this high-risk population is needed. Objective: We aimed to identify the biomarker candidates to distinguish SR from steroid-sensitive (SS) patients using metabolomics approach and to identify the possible molecular mechanism of resistance. Methods: Urine was collected from biopsy-proven FSGS patients... 

    Chemometrics comparison of gas chromatography with mass spectrometry and comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry Daphnia magna metabolic profiles exposed to salinity

    , Article Journal of Separation Science ; Volume 41, Issue 11 , 2018 , Pages 2368-2379 ; 16159306 (ISSN) Parastar, H ; Garreta Lara, E ; Campos, B ; Barata, C ; Lacorte, S ; Tauler, R ; Sharif University of Technology
    Wiley-VCH Verlag  2018
    The performances of gas chromatography with mass spectrometry and of comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry are examined through the comparison of Daphnia magna metabolic profiles. Gas chromatography with mass spectrometry and comprehensive two-dimensional gas chromatography with mass spectrometry were used to compare the concentration changes of metabolites under saline conditions. In this regard, a chemometric strategy based on wavelet compression and multivariate curve resolution–alternating least squares is used to compare the performances of gas chromatography with mass spectrometry and comprehensive two-dimensional gas chromatography with... 

    Possible role for growth hormone in suppressing acylated ghrelin and hunger ratings during and after intermittent exercise of different intensities in obese individuals

    , Article Acta Medica Iranica ; Vol. 52, Issue. 1 , 2014 , pp. 29-37 ; ISSN: 1735-9694 Gholipour, M ; Kordi, M. R ; Taghikhani, M ; Ravasi, A. A ; Gaeini, A. A ; Tabrizi, A ; Sharif University of Technology
    Body weight is influenced by both food intake and energy expenditure. Acylated ghrelin enhances appetite, and its circulating level is suppressed by Growth Hormone. Data on the acylated ghrelin responses to exercise of different intensities in obese individuals are currently not available. This study examined the effects of an intermittent exercise protocol on acylated ghrelin levels and hunger ratings in obese people. Nine inactive male ran on the treadmill at 0900 with progressive intensities of 50, 60, 70, and 80% of VO2max for 10, 10, 5, and 2 min respectively. Blood samples were collected before the exercise at 0845 (-15 min as the resting values), after each workload (10, 23, 31, and... 

    1H NMR based metabolic profiling in Crohn's disease by random forest methodology

    , Article Magnetic Resonance in Chemistry ; Vol. 52, issue. 7 , July , 2014 , p. 370-376 Fathi, F ; Majari-Kasmaee, L ; Mani-Varnosfaderani, A ; Kyani, A ; Rostami-Nejad, M ; Sohrabzadeh, K ; Naderi, N ; Zali, M. R ; Rezaei-Tavirani, M ; Tafazzoli, M ; Arefi-Oskouie, A ; Sharif University of Technology
    The present study was designed to search for metabolic biomarkers and their correlation with serum zinc in Crohn's disease patients. Crohn's disease (CD) is a form of inflammatory bowel disease that may affect any part of the gastrointestinal tract and can be difficult to diagnose using the clinical tests. Thus, introduction of a novel diagnostic method would be a major step towards CD treatment.Proton nuclear magnetic resonance spectroscopy ( 1H NMR) was employed for metabolic profiling to find out which metabolites in the serum have meaningful significance in the diagnosis of CD. CD and healthy subjects were correctly classified using random forest methodology. The classification model for... 

    Disc size markedly influences concentration profiles of intravenously administered solutes in the intervertebral disc: A computational study on glucosamine as a model solute

    , Article European Spine Journal ; Vol. 23, issue. 4 , April , 2014 , p. 715-723 Motaghinasab, S ; Shirazi-Adl, A ; Parnianpour, M ; Urban, J. P. G ; Sharif University of Technology
    Purpose: Tests on animals of different species with large differences in intervertebral disc size are commonly used to investigate the therapeutic efficacy of intravenously injected solutes in the disc. We hypothesize that disc size markedly affects outcome. Methods: Here, using a small non-metabolized molecule, glucosamine (GL) as a model solute, we calculate the influence of disc size on transport of GL into rat, rabbit, dog and human discs for 10 h post intravenous-injection. We used transient finite element models and considered an identical GL supply for all animals. Results: Huge effects of disc size on GL concentration profiles were found. Post-injection GL concentration in the rat... 

    Graphene: Promises, facts, opportunities, and challenges in nanomedicine

    , Article Chemical Reviews ; Volume 113, Issue 5 , 2013 , Pages 3407-3424 ; 00092665 (ISSN) Mao, H. Y ; Laurent, S ; Chen, W ; Akhavan, O ; Imani, M ; Ashkarran, A. A ; Mahmoudi, M ; Sharif University of Technology
    Graphene, a two-dimensional (2D) sheet of sp2-hybridized carbon atoms packed into a honeycomb lattice, has led to an explosion of interest in the field of materials science, physics, chemistry, and biotechnology since the few-layers graphene (FLG) flakes were isolated from graphite in 2004. For an extended search, derivatives of nanomedicine such as biosensing, biomedical, antibacterial, diagnosis, cancer and photothermal therapy, drug delivery, stem cell, tissue engineering, imaging, protein interaction, DNA, RNA, toxicity, and so on were also added. Since carbon nanotubes are normally described as rolled-up cylinders of graphene sheets and the controllable synthesis of nanotubes is well... 

    Characterization of nitrocarburized surface layer on AISI 1020 steel by electrolytic plasma processing in an urea electrolyte

    , Article Journal of Materials Research and Technology ; Volume 2, Issue 3 , 2013 , Pages 213-220 ; 22387854 (ISSN) Karimi Zarchi, M ; Shariat, M.H ; Dehghan, S. A ; Solhjoo, S ; Sharif University of Technology
    Elsevier Editora Ltda  2013
    In this study, electrolytic plasma processing (EPP) was employed for surface nitrocarburizing of AISI 1020 steel in a urea electrolyte, where the substrate samples were connected cathodically to a high-voltage DC current power supply. The structural, mechanical, wear and corrosion properties of the samples treated for 3-5 min were investigated. The results show that the surface layers formed on the samples by this treatment at 220 V have a ferritic nitrocarburizing characteristic which consists of a compound layer and diffusion zone. The surface layers of the treated samples at 240 V consisted of a compound layer, martensitic layer and diffusion zone, respectively, which is a marker of... 

    Multiway investigation of interaction between fluorescence labeled DNA strands and unmodified gold nanoparticles

    , Article Analytical Chemistry ; Volume 84, Issue 15 , July , 2012 , Pages 6603-6610 ; 00032700 (ISSN) Akhlaghi, Y ; Kompany Zareh, M ; Hormozi Nezhad, M. R ; Sharif University of Technology
    ACS  2012
    The single stranded DNA can be adsorbed on the negatively charged surface of gold nanoparticles (AuNPs), but the rigid structure of double stranded DNA prevents it from adsorption. Signal of a tagged single stranded DNA will be quenched by the plasmon effect of the AuNP surface after its adsorption. This phenomenon has been used to study the DNA hybridization and interactions of two complementary 21mer oligonucleotides each tagged with a different fluorescent dye in the presence of 13 nm gold nanoparticles. The DNA strands used in this study belong to the genome of HIV. The obtained rank deficient three-way fluorescence data sets were resolved by both PARAFAC and restricted Tucker3 models.... 

    Thermodynamic properties of aqueous salt containing urea solutions

    , Article Fluid Phase Equilibria ; Volume 325 , July , 2012 , Pages 71-79 ; 03783812 (ISSN) Sadeghi, M ; Held, C ; Samieenasab, A ; Ghotbi, C ; Abdekhodaie, M. J ; Taghikhani, V ; Sadowski, G ; Sharif University of Technology
    Urea and inorganic ions are present in some of the physiological systems, e.g. urine. Understanding the interactions in urea/salt/water is a preliminary step to shed light on more complicated behavior of multi-component physiological systems. State-of-the-art models as well as thermophysical properties can be applied to understand the interactions in these systems. In order to determine such interactions densities, mean ionic activity coefficients (MIACs), osmotic coefficients, and solubility were measured in aqueous solutions of urea and different salts. Densities were determined at temperatures 293.15, 303.15, and 313.15K for urea concentrations up to 3molal and up to 1molal for NaCl.... 

    Pore control in SMA NiTi scaffolds via space holder usage

    , Article Materials Science and Engineering C ; Volume 32, Issue 5 , 2012 , Pages 1266-1270 ; 09284931 (ISSN) Ghasemi, A ; Hosseini, S. R ; Sadrnezhaad, S. K ; Sharif University of Technology
    Porous NiTi shape memory alloy (SMA) was fabricated by sintering of compressed constituent elements pre-mixed with NaCl or urea spacer holders. Effect of spacer to metal volume-ratio (r S) on shape, size, distribution and openness of the voids was probed by optical metallography, X-ray diffraction (XRD) and scanning electron microscopy (SEM). Differential scanning calorimetry (DSC) was used to determine the SMA transformation temperatures. Controllable void geometry helping osteoblast proliferation and bone cell growth was gained by addition of the spacers. At r S = 0.7, percentage of the open pores reached 52% while at r S = 1.43, interconnected pores with 200 to 500 μm diameter were... 

    Naphtha hydrodesulfurization over micro and nanostructure MoO3 catalysts

    , Article Scientia Iranica ; Volume 18, Issue 3 C , June , 2011 , Pages 479-485 ; 10263098 (ISSN) Parviz, D ; Kazemeini, M ; Rashidi, A. M ; Jozani, Kh. J ; Sharif University of Technology
    Highly active unsupported MoO3 and Co -MoO3 catalysts were prepared by a simple solution reaction method in the presence of different organic additives. Catalysts were characterized by the XRD, BET surface area measurement, SEM and TEM techniques. Results suggested that utilizing different additives produced different morphologies OfMoO3 microstructures. Optimizing reaction conditions was found to produce more active molybdenum oxide nanoparticles when urea and PEG200 additives were utilized. XRD and TEM results indicated crystal growth restriction after cobalt incorporation. Catalytic activities of prepared materials were evaluated in the hydrodesulfurization reaction of Naphtha. Products... 

    A study of Acidithiobacillus Ferrooxidans DSMZ 583 Adaptation to Heavy Metals

    , Article Iranian Journal of Biotechnology ; Volume 9, Issue 2 , 2011 , Pages 133-144 ; 17283043 (ISSN) Yaghmaei, S ; Ghobadi, Z ; Sharif University of Technology
    In this study the ability of Acidithiobacillus ferrooxi-dans, with regard to the biorecovery of heavy metals in shake flask has been investigated. Adaptation experiments with the single metal ions Ni, Co, V, Mo, W and a mixture of the first four metal ions in the medium was developed through serial sub-culturing. Adaptation showed that A. ferrooxidans could tolerate up to 2.3 g/l Ni, 1.4 g/l Co, 1.4 g/l V, 0.045 g/l Mo and 0.005 g/l W, singly. In the presence of multi-metals considering a mixture of Ni-Co-V-Mo, the bacteria was able to tolerate up to 1.5 g/l Ni, 0.8 g/l Co, 0.8 g/l V and 0.05 g/l Mo in steps of 50-100 mg/l for Ni, Co and V, while for Mo and W with increments in concentration...