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A smart tri-layered nanofibrous hydrogel thin film with controlled release of dual drugs for chemo-thermal therapy of breast cancer
, Article Journal of Drug Delivery Science and Technology ; Volume 76 , 2022 ; 17732247 (ISSN) ; Mohammadi Ziarani, G ; Badiei, A ; Pourjavadi, A ; Kiani, M ; Sharif University of Technology
Editions de Sante
2022
Abstract
A tri-layered nanofibrous hydrogel thin film with temperature sensitivity is introduced as a new controlled drug release system to treat breast cancer. A simultaneous heat generation with the tunable release of dual drugs is observed in response to visible light radiation. A tri-layered nanofibrous sheet was fabricated through sequential electrospinning the blends of Au@Chit@DOX-loaded poly(N-isopropylacrylamide-co-N-methylol acrylamide) (poly (NIPAAm-co-NMA)) and Curcumin-loaded poly (vinyl alcohol) (Cu-loaded PVA), where the Cu-loaded PVA nanofibers (NFs) are sandwiched between two layers of Au@Chit@DOX-loaded poly (NIPAAm-co-NMA) NFs. After thermal crosslinking of the tri-layered...
Apoptotic and anti-apoptotic genes transcripts patterns of graphene in mice
, Article Materials Science and Engineering C ; Volume 71 , 2017 , Pages 460-464 ; 09284931 (ISSN) ; Hashemi, E ; Akhavan, O ; Shamsara, M ; Hashemi, M ; Farmany, A ; Daliri Joupari, M ; Sharif University of Technology
Elsevier Ltd
2017
Abstract
Recent studies showed that a large amount of graphene oxide accumulated in kidney and liver when it injected intravenously. Evaluation of lethal and apoptosis gene expression in these tissues, which are under stress is very important. In this paper the in vivo dose-dependent effects of graphene oxide and reduced graphene oxide nanoplatelets on kidney and liver of mice were studied. Balb/C mice were treated by 20 mg/kg body weight of nanoplatelets. Molecular biology analysis showed that graphene nanoplatelets injected intravenously lead to overexpression of BAX gene in both kidney and liver tissues (P ≥ 0.01). In addition these nanoparticles significantly increase BCL2 gene expression in both...
Antitumor effect of therapeutic HPV DNA vaccines with chitosan-based nanodelivery systems
, Article Journal of Biomedical Science ; Vol. 21, issue. 1 , July , 2014 ; ISSN: 10217770 ; Ghaemi, A ; Gorji, A ; Kalhor, H. R ; Sajadian, A ; Tabarraei, A ; Moradi, A ; Atyabi, F ; Kelishadi, M ; Sharif University of Technology
Abstract
Cervical cancer is the second-most-common cause of malignancies in women worldwide, and the oncogenic activity of the human papilloma virus types (HPV) E7 protein has a crucial role in anogenital tumors. In this study, we have designed a therapeutic vaccine based on chitosan nanodelivery systems to deliver HPV-16 E7 DNA vaccine, considered as a tumor specific antigen for immunotherapy of HPV-associated cervical cancer. We have developed a Nano-chitosan (NCS) as a carrier system for intramuscular administration using a recombinant DNA vaccine expressing HPV-16 E7 (NCS-DNA E7 vaccine). NCS were characterized in vitro for their gene transfection ability. Results: The transfection of CS-pEGFP...
A novel formulation of simvastatin nanoemulsion gel for infected wound therapy: In vitro and in vivo assessment
, Article Journal of Drug Delivery Science and Technology ; Volume 72 , 2022 ; 17732247 (ISSN) ; Ghaffari, A ; Keramati, M ; Ahmadi, S ; Dizaji, S ; Moayer, F ; Akbarzadeh, I ; Abazari, M ; razzaghi abyaneh, M ; Bakhshandeh, H ; Sharif University of Technology
Editions de Sante
2022
Abstract
Simvastatin, a well-known antihyperlipidemic drug, has antibacterial activity against a broad range of bacteria, especially Staphylococcus aureus. In present study, a nanoemulsion gel-based formulation containing Simvastatin was developed for infected wound therapy. Therefore, different formulations of Simvastatin nanoemulsion were prepared. Based on droplet size, polydispersity index and zeta potential, the best nanoemulsion formulation containing Simvastatin was selected for development of nanoemulsion gel formulation of drug using carbomer 934 as gelling agent. Thermodynamic stability of Simvastatin nanoemulsion was assessed at different conditions. The in vitro antibacterial activity...
An in vitro study of bare and poly(ethylene glycol)-co-fumarate-coated superparamagnetic iron oxide nanoparticles: A new toxicity identification procedure
, Article Nanotechnology ; Volume 20, Issue 22 , 2009 ; 09574484 (ISSN) ; Simchi, A ; Imani, M ; Milani, A. S ; Stroeve, P ; Sharif University of Technology
2009
Abstract
As the use of superparamagnetic iron oxide nanoparticles (SPION) in biomedical applications increases (e.g. for targeting drug delivery and imaging), patients are likely to be exposed to products containing SPION. Despite their high biomedical importance, toxicity data for SPION are limited to date. The aim of this study is to investigate the cytotoxicity of SPION and its ability to change cell medium components. Bare and poly(ethylene glycol)-co-fumarate (PEGF)-coated SPION with narrow size distributions were synthesized. The particles were prepared by co-precipitation using ferric and ferrous salts with a molar Fe3+/Fe2+ ratio of 2. Dulbecco's modified Eagle's medium (DMEM) and primary...
An improved synthesis and preliminary biodistribution study of a technetium-99m-labeled2-amino-2-deoxy(thioacetyl)-D-glucose complex ([ 99mTc]-TA-DG) as a tumor imaging agent
, Article Iranian Journal of Nuclear Medicine ; Volume 15, Issue 28 , 2007 , Pages 43-48 ; 16812824 (ISSN) ; Sadeghzadeh, M ; Charkhlooie, G ; Haghir Ebrahimabadi, K ; Saeedi, M. R ; Sharif University of Technology
2007
Abstract
Introduction: This report describes the synthesis of 2-Amino-2-deoxy(S- benzoylthioacetyl)-D-glucose (S-Bz-TA-DG), radiolabeled with [ 99mTc(CO)3(OH2)3]+ complex with a procedure including deprotection of the benzoyl group, characterization by HPLC using a C18 reverse phase column and preliminary biodistribution study in normal mice. Methods: [99mTc(CO) 3(H2O)3]+ complex was used to label TA-DG with 99mTc. This complex was prepared using up to 46 mCi of Na99mTcO4 in 1mL saline. The radiochemical purity (>95%) was determined by TLC in normal saline solution as the mobile phase. Radio-HPLC analysis of [99mTc]-(TA-DG) at pH=9.5-10, revealed that labeling with 99mTc resulted in the formation of...
A new approach for the in vitro identification of the cytotoxicity of superparamagnetic iron oxide nanoparticles
, Article Colloids and Surfaces B: Biointerfaces ; Volume 75, Issue 1 , 2010 , Pages 300-309 ; 09277765 (ISSN) ; Simchi, A ; Imani, M ; Shokrgozar, M. A ; Milani, A. S ; Häfeli, U. O ; Stroeve, P ; Sharif University of Technology
Abstract
Superparamagnetic iron oxide nanoparticles (SPIONs) are increasingly used in medical applications, such as targeting delivery and imaging. In the future, patients are more likely to be exposed to pharmaceutical products containing such particles. The study of toxicity of SPIONs has become of great importance in recent years, although the published data in this arena is limited. The aim of the present work is to investigate the cytotoxicity of SPIONs and the effect of the particles on the cell medium components. For this purpose, uncoated and polyvinyl alcohol (PVA) coated SPIONs with narrow size distribution were synthesized via a well-known coprecipitation method. The mouse fibroblast cell...
Alginate-based multifunctional films incorporated with sulfur quantum dots for active packaging applications
, Article Colloids and Surfaces B: Biointerfaces ; Volume 215 , 2022 ; 09277765 (ISSN) ; Priyadarshi, R ; Rhim, J. W ; Lotfali, E ; Bagheri, R ; Pircheraghi, G ; Sharif University of Technology
Elsevier B.V
2022
Abstract
Sulfur quantum dots (SQDs) were fabricated using a facile hydrothermal method and used for the preparation of functional food packaging film and compared the properties with other sulfur-based fillers like elemental sulfur (ES) and sulfur nanoparticles (SNP). The SQDs have an average size of 5.3 nm and were very stable in aqueous suspension. Unlike other sulfur-based fillers, the SQD showed high antioxidant, antibacterial and antifungal activity, but no cytotoxicity was found for L929 mouse fibroblasts even after long-term exposure of 48 h. When sulfur-based fillers were added to the alginate film, SQD was more evenly dispersed in the polymer matrix than SNP and ES. The addition of SQD to...
A hydrogen-bonded extracellular matrix-mimicking bactericidal hydrogel with radical scavenging and hemostatic function for ph-responsive wound healing acceleration
, Article Advanced Healthcare Materials ; Volume 10, Issue 3 , 2021 ; 21922640 (ISSN) ; Correia, A ; Hasany, M ; Figueiredo, P ; Dobakhti, F ; Eskandari, M. R ; Hosseini, S.H ; Abiri, R ; Khorshid, S ; Hirvonen, J ; Santos, H. A ; Shahbazi, M. A ; Sharif University of Technology
Wiley-VCH Verlag
2021
Abstract
Generation of reactive oxygen species, delayed blood clotting, prolonged inflammation, bacterial infection, and slow cell proliferation are the main challenges of effective wound repair. Herein, a multifunctional extracellular matrix-mimicking hydrogel is fabricated through abundant hydrogen bonding among the functional groups of gelatin and tannic acid (TA) as a green chemistry approach. The hydrogel shows adjustable physicochemical properties by altering the concentration of TA and it represents high safety features both in vitro and in vivo on fibroblasts, red blood cells, and mice organs. In addition to the merit of facile encapsulation of cell proliferation-inducing hydrophilic drugs,...
A hydrogen-bonded extracellular matrix-mimicking bactericidal hydrogel with radical scavenging and hemostatic function for ph-responsive wound healing acceleration
, Article Advanced Healthcare Materials ; Volume 10, Issue 3 , 2021 ; 21922640 (ISSN) ; Correia, A ; Hasany, M ; Figueiredo, P ; Dobakhti, F ; Eskandari, M. R ; Hosseini, S. H ; Abiri, R ; Khorshid, S ; Hirvonen, J ; Santos, H. A ; Shahbazi, M. A ; Sharif University of Technology
Wiley-VCH Verlag
2021
Abstract
Generation of reactive oxygen species, delayed blood clotting, prolonged inflammation, bacterial infection, and slow cell proliferation are the main challenges of effective wound repair. Herein, a multifunctional extracellular matrix-mimicking hydrogel is fabricated through abundant hydrogen bonding among the functional groups of gelatin and tannic acid (TA) as a green chemistry approach. The hydrogel shows adjustable physicochemical properties by altering the concentration of TA and it represents high safety features both in vitro and in vivo on fibroblasts, red blood cells, and mice organs. In addition to the merit of facile encapsulation of cell proliferation-inducing hydrophilic drugs,...