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    Estimation of nitrogen content in cucumber plant (Cucumis sativus L.) leaves using hyperspectral imaging data with neural network and partial least squares regressions

    , Article Chemometrics and Intelligent Laboratory Systems ; Volume 217 , 2021 ; 01697439 (ISSN) Sabzi, S ; Pourdarbani, R ; Rohban, M. H ; García Mateos, G ; Arribas, J. I ; Sharif University of Technology
    Elsevier B.V  2021
    In recent years, farmers have often mistakenly resorted to overuse of chemical fertilizers to increase crop yield. However, excessive consumption of fertilizers might lead to severe food poisoning. If nutritional deficiencies are detected early, it can help farmers to design better fertigation practices before the problem becomes unsolvable. The aim of this study is to predict the amount of nitrogen (N) content (mg l−1) in cucumber (Cucumis sativus L., var. Super Arshiya-F1) plant leaves using hyperspectral imaging (HSI) techniques and three different regression methods: a hybrid artificial neural networks-particle swarm optimization (ANN-PSO); partial least squares regression (PLSR); and... 

    Effect of physico-chemical properties of nanoparticles on their intracellular uptake

    , Article International Journal of Molecular Sciences ; Volume 21, Issue 21 , 2020 , Pages 1-20 Sabourian, P ; Yazdani, G ; Ashraf, S. S ; Frounchi, M ; Mashayekhan, S ; Kiani, S ; Kakkar, A ; Sharif University of Technology
    MDPI AG  2020
    Cellular internalization of inorganic, lipidic and polymeric nanoparticles is of great significance in the quest to develop effective formulations for the treatment of high morbidity rate diseases. Understanding nanoparticle–cell interactions plays a key role in therapeutic interventions, and it continues to be a topic of great interest to both chemists and biologists. The mechanistic evaluation of cellular uptake is quite complex and is continuously being aided by the design of nanocarriers with desired physico-chemical properties. The progress in biomedicine, including enhancing the rate of uptake by the cells, is being made through the development of structure–property relationships in... 

    Stimuli-responsive chitosan as an advantageous platform for efficient delivery of bioactive agents

    , Article Journal of Controlled Release ; Volume 317 , 2020 , Pages 216-231 Sabourian, P ; Tavakolian, M ; Yazdani, H ; Frounchi, M ; van de Ven, T. G. M ; Maysinger, D ; Kakkar, A ; Sharif University of Technology
    Elsevier B.V  2020
    Despite a diverse range of active pharmaceutical agents currently at our disposal, high morbidity rate diseases continue to pose a major health crisis globally. One of the important parameters in this regard is the controlled cargo delivery at desired sites. Among a variety of synthetic and natural macromolecular systems, chitosan, an abundant biopolymer, offers a platform for tailored architectures that could have high loading capacity of cargo, target and deliver. Stimuli directed accumulation of vehicles and drug release is an area of direct relevance to biomedical applications. In this review, we highlight essential characteristics of modified chitosan that present themselves for... 

    Inactivation methods for whole influenza vaccine production

    , Article Reviews in Medical Virology ; Volume 29, Issue 6 , 2019 ; 10529276 (ISSN) Sabbaghi, A ; Miri, S. M ; Keshavarz, M ; Zargar, M ; Ghaemi, A ; Sharif University of Technology]
    John Wiley and Sons Ltd  2019
    Despite tremendous efforts toward vaccination, influenza remains an ongoing global threat. The induction of strain-specific neutralizing antibody responses is a common phenomenon during vaccination with the current inactivated influenza vaccines, so the protective effect of these vaccines is mostly strain-specific. There is an essential need for the development of next-generation vaccines, with a broad range of immunogenicity against antigenically drifted or shifted influenza viruses. Here, we evaluate the potential of whole inactivated vaccines, based on chemical and physical methods, as well as new approaches to generate cross-protective immune responses. We also consider the mechanisms by... 

    Dna-Rna hybrid (R-loop): From a unified picture of the mammalian telomere to the genome-wide profile

    , Article Cells ; Volume 10, Issue 6 , 2021 ; 20734409 (ISSN) Rassoulzadegan, M ; Sharifi Zarchi, A ; Kianmehr, L ; Sharif University of Technology
    MDPI  2021
    Local three-stranded DNA/RNA hybrid regions of genomes (R-loops) have been detected either by binding of a monoclonal antibody (DRIP assay) or by enzymatic recognition by RNaseH. Such a structure has been postulated for mouse and human telomeres, clearly suggested by the identification of the complementary RNA Telomeric repeat-containing RNA “TERRA”. However, the tremendous disparity in the information obtained with antibody-based technology drove us to investigate a new strategy. Based on the observation that DNA/RNA hybrids in a triplex complex genome co-purify with the double-stranded chromosomal DNA fraction, we developed a direct preparative approach from total protein-free cellular... 

    Optimization of operating parameters and rate of uranium bioleaching from a low-grade ore

    , Article Journal of Radioanalytical and Nuclear Chemistry ; Vol. 301, issue. 2 , 2014 , pp. 341-350 ; ISSN: 02365731 Rashidi, A ; Roosta-Azad, R ; Safdari, S. J ; Sharif University of Technology
    In this study the bioleaching of a low-grade uranium ore containing 480 ppm uranium has been reported. The studies involved extraction of uranium using Acidithiobacillus ferrooxidans derived from the uranium mine samples. The maximum specific growth rate (μ max) and doubling time (t d) were obtained 0.08 h-1 and 8.66 h, respectively. Parameters such as Fe2+ concentration, particle size, temperature and pH were optimized. The effect of pulp density (PD) was also studied. Maximum uranium bio-dissolution of 100 ± 5 % was achieved under the conditions of pH 2.0, 5 % PD and 35 °C in 48 h with the particles of d 80 = 100 μm. The optimum concentration of supplementary Fe2+ was dependent to the PD.... 

    Natural compounds for skin tissue engineering by electrospinning of nylon-Beta vulgaris

    , Article ASAIO Journal ; Volume 64, Issue 2 , 2018 , Pages 261-269 ; 10582916 (ISSN) Ranjbarvan, P ; Mahmoudifard, M ; Kehtari, M ; Babaie, A ; Hamedi, S ; Mirzaei, S ; Soleimani, M ; Hosseinzadeh, S ; Sharif University of Technology
    Lippincott Williams and Wilkins  2018
    Natural compounds containing polysaccharide ingredients have been employed as candidates for treatment of skin tissue. Herein, for the first time, electrospinning setup was proposed to fabricate an efficient composite nanofibrous structure of Beta vulgaris (obtained from Beet [Chenopodiaceae or Amaranthaceae]) belonged to polysaccharides and an elastic polymer named nylon 66 for skin tissue engineering. Both prepared scaffolds including noncomposite and composite types were studied by Scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy, mechanical assay, and contact angle. Scanning electron microscope examinations have approved the uniform and homogeneous... 

    Design and fabrication of an electrochemical aptasensor using Au nanoparticles/carbon nanoparticles/cellulose nanofibers nanocomposite for rapid and sensitive detection of Staphylococcus aureus

    , Article Bioelectrochemistry ; Volume 123 , 2018 , Pages 70-76 ; 15675394 (ISSN) Ranjbar, S ; Shahrokhian, S ; Sharif University of Technology
    Elsevier B.V  2018
    Since that pathogenic bacteria are major threats to human health, this paper describes the fabrication of an effective and durable sensing platform based on gold nanoparticles/carbon nanoparticles/cellulose nanofibers nanocomposite (AuNPs/CNPs/CNFs) at the surface of glassy carbon electrode for sensitive and selective detection of Staphylococcus aureus (S. aureus). The AuNPs/CNPs/CNFs nanocomposite with the high surface area, excellent conductivity, and good biocompatibility was used for self-assembled of the thiolated specific S. aureus aptamer as a sensing element. The surface morphology of AuNPs/CNPs/CNFs nanocomposite was characterized with field emission scanning electron microscopy... 

    New gasochromic system: Nanoparticles in liquid

    , Article Journal of Nanoparticle Research ; Volume 14, Issue 4 , March , 2012 ; 13880764 (ISSN) Ranjbar, M ; Kalhori, H ; Mahdavi, S. M ; Zad, A. I ; Sharif University of Technology
    In this study, WO 3 nanocrystallites were first produced by laser ablation of W target in deionised water. To synthesize palladium, a PdCl 2 solution (0.2 g/L) was added to the liquid. Transmission electron microscope revealed successful synthesis of tungsten oxide nanocrystallites along with the production of Pd and core-shell Pd/WO3 nanoparticles. Gasochromic behavior was examined by hydrogen bubbling into Pd/WO 3 liquid in which a transition to blue absorbing state was observed. Optical absorption spectra of the colored liquid represented different sharp small polaron absorbing peaks below 3 eV and the peaks intensity was observed to be varied with Pd:WO 3 ratio. Time variations of... 

    Ag-doped magnetic metal organic framework as a novel nanostructured material for highly efficient antibacterial activity

    , Article Environmental Research ; Volume 188 , 2020 Rahmati, Z ; Abdi, J ; Vossoughi, M ; Alemzadeh, I ; Sharif University of Technology
    Academic Press Inc  2020
    In the last decades, numerous attempts have been made to prevent microbial pollution spreading, using antibacterial agents. Zeolitic imidazolate framework-8 (ZIF-8) belongs to a subgroup of metal organic frameworks (MOFs) merits of attention due to the zinc ion clusters and its effective antibacterial activity. In this work, Ag-doped magnetic microporous γ-Fe2O3@SiO2@ZIF-8-Ag (FSZ-Ag) was successfully synthesized by a facile methodology in room temperature and used as an antibacterial agent against the growth of the Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria. Several characterization methods were applied to analyze the properties of the materials, and the... 

    A continuous flow microfluidic device based on contactless dielectrophoresis for bioparticles enrichment

    , Article Electrophoresis ; Volume 39, Issue 3 , 2018 , Pages 445-455 ; 01730835 (ISSN) Rahmani, A ; Mohammadi, A. A ; Kalhor, H. R ; Sharif University of Technology
    Wiley-VCH Verlag  2018
    In recent years, applications of dielectrophoresis-based platforms have been recognized as effective and dependable approach to separate cells and bioparticles, suspended in different carrier fluids, based on particle size and electrical properties. In this study, a microfluidic device was fabricated by an unprecedented electrode pattern, and several experiments were performed to enrich samples including either of yeast, Escherichia coli, or latex particles. A chemical deposition-based method was employed for fabrication of microelectrodes, inducing nonuniform electric field required for dielectrophoresis-based separation. One major advantage of our employed method is low fabrication cost,... 

    Genome annotation and comparative genomic analysis of Bacillus subtilis MJ01, a new bio-degradation strain isolated from oil-contaminated soil

    , Article Functional and Integrative Genomics ; Volume 18, Issue 5 , 2018 , Pages 533-543 ; 1438793X (ISSN) Rahimi, T ; Niazi, A ; Deihimi, T ; Taghavi, S. M ; Ayatollahi, S ; Ebrahimie, E ; Sharif University of Technology
    Springer Verlag  2018
    One of the main challenges in elimination of oil contamination from polluted environments is improvement of biodegradation by highly efficient microorganisms. Bacillus subtilis MJ01 has been evaluated as a new resource for producing biosurfactant compounds. This bacterium, which produces surfactin, is able to enhance bio-accessibility to oil hydrocarbons in contaminated soils. The genome of B. subtilis MJ01 was sequenced and assembled by PacBio RS sequencing technology. One big contig with a length of 4,108,293 bp without any gap was assembled. Genome annotation and prediction of gene showed that MJ01 genome is very similar to B. subtilis spizizenii TU-B-10 (95% similarity). The comparison... 

    Optimization of culture medium and modeling of curdlan production from Paenibacillus polymyxa by RSM and ANN

    , Article International Journal of Biological Macromolecules ; Vol. 70, issue , Jul , 2014 , p. 463-473 Rafigh, S. M ; Yazdi, A. V ; Vossoughi, M ; Safekordi, A. A ; Ardjmand, M ; Sharif University of Technology
    Paenibacillus polymyxa ATCC 21830 was used for the production of curdlan gum for first time. A Box-Behnken experimental design was applied to optimize six variables of batch fermentation culture each at three levels. Statistical analyses were employed to investigate the direct and interactive effects of variables on curdlan production. Optimum cultural conditions were temperature (50. °C), pH (7), fermentation time (96. h), glucose (100. g/L), yeast extract (3. g/L) and agitation speed (150. rpm). The yield of curdlan production was 6.89. g/L at optimum condition medium. Response surface methodology (RSM) and artificial neural network (ANN) were used to model cultural conditions of curdlan... 

    Chemometrics-assisted effect-directed analysis of crude and refined oil using comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry

    , Article Environmental Science and Technology ; Vol. 48, issue. 5 , 2014 , pp. 3074-3083 ; ISSN: 0013936X Radovic, J. R ; Thomas, K. V ; Parastar, H ; Diez, S ; Tauler, R ; Bayona, J. M ; Sharif University of Technology
    An effect-directed analysis (EDA) of fresh and artificially weathered (evaporated, photooxidized) samples of North Sea crude oil and residual heavy fuel oil is presented. Aliphatic, aromatic, and polar oil fractions were tested for the presence of aryl hydrocarbon receptor (AhR) agonist and androgen receptor (AR) antagonist, demonstrating for the first time the AR antagonist effects in the aromatic and, to a lesser extent, polar fractions. An extension of the typical EDA strategy to include an N-way partial least-squares (N-PLS) model capable of relating the comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC × GC-TOFMS) data set to the bioassay data... 

    Core flooding tests to investigate the effects of IFT reduction and wettability alteration on oil recovery during MEOR process in an Iranian oil reservoir

    , Article Applied Microbiology and Biotechnology ; Volume 97, Issue 13 , July , 2013 , Pages 5979-5991 ; 01757598 (ISSN) Rabiei, A ; Sharifinik, M ; Niazi, A ; Hashemi, A ; Ayatollahi, S ; Sharif University of Technology
    Microbial enhanced oil recovery (MEOR) refers to the process of using bacterial activities for more oil recovery from oil reservoirs mainly by interfacial tension reduction and wettability alteration mechanisms. Investigating the impact of these two mechanisms on enhanced oil recovery during MEOR process is the main objective of this work. Different analytical methods such as oil spreading and surface activity measurements were utilized to screen the biosurfactant-producing bacteria isolated from the brine of a specific oil reservoir located in the southwest of Iran. The isolates identified by 16S rDNA and biochemical analysis as Enterobacter cloacae (Persian Type Culture Collection (PTCC)... 

    Carbosilane dendrimers: Drug and gene delivery applications

    , Article Journal of Drug Delivery Science and Technology ; Volume 59 , 2020 Rabiee, N ; Ahmadvand, S ; Ahmadi, S ; Fatahi, Y ; Dinarvand, R ; Bagherzadeh, M ; Rabiee, M ; Tahriri, M ; Tayebi, L ; Hamblin, M. R ; Sharif University of Technology
    Editions de Sante  2020
    Carbosilane dendrimers are a particular type of dendrimer structure that has been used as delivery vehicles for drugs and nucleic acids. They have a defined structure according to their generation number, and their terminal groups can be rendered cationic or anionic. The cationic charges can address the limitation of electrostatic repulsion between the negatively charged phosphate groups of nucleic acids and negatively charged cell membranes. Specific drugs can be loaded into the central part of the dendrimer or attached at the exterior, and the overall positive charge may improve the efficacy of anti-inflammatory drugs. One promising feature of dendrimers is their non-toxicity both in vitro... 

    Recent advances in porphyrin-based nanocomposites for effective targeted imaging and therapy

    , Article Biomaterials ; Volume 232 , 2020 Rabiee, N ; Tavakkoli Yaraki, M ; Mokhtari Garakani, S ; Mokhtari Garakani, S ; Ahmadi, S ; Lajevardi, A ; Bagherzadeh, M ; Rabiee, M ; Tayebi, L ; Tahriri, M ; Hamblin, M. R ; Sharif University of Technology
    Elsevier Ltd  2020
    Porphyrins are organic compounds that continue to attract much theoretical interest, and have been called the “pigments of life”. They have a wide role in photodynamic and sonodynamic therapy, along with uses in magnetic resonance, fluorescence and photoacoustic imaging. There is a vast range of porphyrins that have been isolated or designed, but few of them have real clinical applications. Due to the hydrophobic properties of porphyrins, and their tendency to aggregate by stacking of the planar molecules they are difficult to work with in aqueous media. Therefore encapsulating them in nanoparticles (NPs) or attachment to various delivery vehicles have been used to improve delivery... 

    Biosynthesis of copper oxide nanoparticles with potential biomedical applications

    , Article International Journal of Nanomedicine ; Volume 15 , 2020 , Pages 3983-3999 Rabiee, N ; Bagherzadeh, M ; Kiani, M ; Ghadiri, A. M ; Etessamifar, F ; Jaberizadeh, A. H ; Shakeri, A ; Sharif University of Technology
    Dove Medical Press Ltd  2020
    Introduction: In recent years, the use of cost-effective, multifunctional, environmentally friendly and simple prepared nanomaterials/nanoparticles have been emerged considerably. In this manner, different synthesizing methods were reported and optimized, but there is still lack of a comprehensive method with multifunctional properties. Materials and Methods: In this study, we aim to synthesis the copper oxide nanoparticles using Achillea millefolium leaf extracts for the first time. Catalytic activity was investigated by in situ azide alkyne cycloaddition click and also A3 coupling reaction, and optimized in terms of temperature, solvent, and time of the reaction. Furthermore, the... 

    Diatoms with invaluable applications in nanotechnology, biotechnology, and biomedicine: Recent advances

    , Article ACS Biomaterials Science and Engineering ; Volume 7, Issue 7 , 2021 , Pages 3053-3068 ; 23739878 (ISSN) Rabiee, N ; Khatami, M ; Jamalipour Soufi, G ; Fatahi, Y ; Iravani, S ; Varma, R. S ; Sharif University of Technology
    American Chemical Society  2021
    Diatoms are unicellular microalga found in soil and almost every aquatic environment (marine and fresh water). Biogenic silica and diatoms are attractive for biotechnological and industrial applications, especially in the field of biomedicine, industrial/synthetic manufacturing processes, and biomedical/pharmaceutical sciences. Deposition of silica by diatoms allows them to create micro- or nanoscale structures which may be utilized in nanomedicine and especially in drug/gene delivery. Diatoms with their unique architectures, good thermal stability, suitable surface area, simple chemical functionalization/modification procedures, ease of genetic manipulations, optical/photonic... 

    Polymeric nanoparticles for nasal drug delivery to the brain: relevance to alzheimer's disease

    , Article Advanced Therapeutics ; Volume 4, Issue 3 , 2021 ; 23663987 (ISSN) Rabiee, N ; Ahmadi, S ; Afshari, R ; Khalaji, S ; Rabiee, M ; Bagherzadeh, M ; Fatahi, Y ; Dinarvand, R ; Tahriri, M ; Tayebi, L ; Hamblin, M. R ; Webster, T. J ; Sharif University of Technology
    Blackwell Publishing Ltd  2021
    Currently, Alzheimer's disease (AD) accounts for more than half of all dementia cases. Although genetics, age, and environmental factors affect the disease, the cause of AD is not yet fully known. Various drugs have been proposed for the prevention and treatment of AD, but the delivery of these therapeutic agents to the brain is difficult. The blood–brain barrier prevents systemic drugs from accessing the central nervous system and designing a suitable system to overcome this barrier has attracted much attention. The intranasal pathway, given its proximity to the brain, provides a great opportunity for drug delivery. Understanding the physiological characteristics of the nose can be useful...