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scaffolds--biology
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Fabrication and properties of polycaprolactone composites containing calcium phosphate-based ceramics and bioactive glasses in bone tissue engineering: a review
, Article Polymer Reviews ; Volume 58, Issue 1 , 2018 , Pages 164-207 ; 15583724 (ISSN) ; Tajbakhsh, S ; Shojaei, A ; Sharif University of Technology
Taylor and Francis Inc
2018
Abstract
Polycaprolactone (PCL) is a bioresorbable and biocompatible polymer that has been widely used in long-term implants and controlled drug release applications. However, when it comes to tissue engineering, PCL suffers from some shortcomings such as slow degradation rate, poor mechanical properties, and low cell adhesion. The incorporation of calcium phosphate-based ceramics and bioactive glasses into PCL has yielded a class of hybrid biomaterials with remarkably improved mechanical properties, controllable degradation rates, and enhanced bioactivity that are suitable for bone tissue engineering. This review presents a comprehensive study on recent advances in the fabrication and properties of...
Chitosan-gelatin sheets as scaffolds for muscle tissue engineering
, Article Artificial Cells, Nanomedicine and Biotechnology ; Volume 43, Issue 2 , Nov , 2015 , Pages 124-132 ; 21691401 (ISSN) ; Mashayekhan, S ; Nazaripouya, A ; Naji, M ; Hunkeler, D ; Rajabi Zeleti, S ; Sharifiaghdas, F ; Sharif University of Technology
Informa Healthcare
2015
Abstract
Hydrogels made of natural polymers [chitosan (CS) and gelatin (G)] have been prepared having mechanical properties similar to those of muscle tissues. In this study, the effect of polymer concentration and scaffold stiffness on the behavior of seeded muscle-derived cells (MDCs) on the CS-G hydrogel sheets has been evaluated. Both variables were found to be important in cell viability. Viability was assessed by observation of the cell morphology after 1 day as well as a 14-day MTT assay. The CS-G hydrogels were characterized using Fourier transform infrared (FTIR) analysis, which revealed evidences of strong intermolecular interactions between CS and G. Hydrogel samples with intermediate...
A porous hydrogel-electrospun composite scaffold made of oxidized alginate/gelatin/silk fibroin for tissue engineering application
, Article Carbohydrate Polymers ; Volume 245 , 2020 ; Alemzadeh, I ; Vossoughi, M ; Sharif University of Technology
Elsevier Ltd
2020
Abstract
In the article, a bilayer nanocomposite scaffold made of oxidized alginate (OAL), gelatin (G), and silk fibroin (SF) has been prepared via combining electrospinning, in situ gas foaming, in situ crosslinking and freeze drying methods. The physicochemical and mechanical properties, as well as thermal stability of the proposed composite, have been investigated by SEM, FTIR, XRD, tensile, and TGA analysis. The data indicate that structure and degree of crosslinking play a vital role in adjusting the physical and mechanical properties of composite scaffolds. Further, the authors find a favorable adipose-derived mesenchymal stem cell's (AMSC) attachment and distribution within this novel...
Hybrid silk fibroin–gelatin nanofibrous sheet for drug delivery and regenerative medicine: In-vitro characterization and controlled release of simvastatin/protein
, Article Polymers for Advanced Technologies ; 2020 ; Alemzadeh, I ; Vossoughi, M ; Sharif University of Technology
John Wiley and Sons Ltd
2020
Abstract
Blend drug-loading method in electrospun scaffolds has gained much attention as a cost-effective and simple delivery system in regenerative medicine. However, it has some drawbacks, such as the burst release of encapsulated drugs and denaturing active agents in harsh organic solvents. In this study, a new silk fibroin-gelatin (SF–G) fibrous sheet has been introduced as an engineered scaffold and a straightforward drug delivery system for skin tissue engineering applications. The hybrid sheets have been prepared via co-electrospinning and in-situ crosslinking methods without corrosive solvents and toxic crosslinking agents. To evaluate the proposed scaffold as a controlled release system, the...
In-situ crosslinking of electrospun gelatin-carbodiimide nanofibers: fabrication, characterization, and modeling of solution parameters
, Article Chemical Engineering Communications ; 2020 ; Alemzadeh, I ; Vossoughi, M ; Shamloo, A ; Sharif University of Technology
Taylor and Francis Ltd
2020
Abstract
This work has focused on in-situ crosslinking of gelatin (G) to produce electrospun scaffold with improved fiber morphology retention and mechanical properties. As per this approach, we prepared G nanofibers through mixing G, 1-ethyl-3-(3 dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) in the new solvent system. Response surface methodology (RSM) was employed to study the influence of solution parameters on fiber diameter. The morphological structure was examined, and the appropriate level of setting to obtain smooth fibers with a favorable diameter was reported. Results revealed using EDC/NHS for in-situ crosslinking improves the mechanical properties...
Hybrid silk fibroin–gelatin nanofibrous sheet for drug delivery and regenerative medicine: In-vitro characterization and controlled release of simvastatin/protein
, Article Polymers for Advanced Technologies ; Volume 32, Issue 3 , 2021 , Pages 1333-1344 ; 10427147 (ISSN) ; Alemzadeh, I ; Vossoughi, M ; Sharif University of Technology
John Wiley and Sons Ltd
2021
Abstract
Blend drug-loading method in electrospun scaffolds has gained much attention as a cost-effective and simple delivery system in regenerative medicine. However, it has some drawbacks, such as the burst release of encapsulated drugs and denaturing active agents in harsh organic solvents. In this study, a new silk fibroin-gelatin (SF–G) fibrous sheet has been introduced as an engineered scaffold and a straightforward drug delivery system for skin tissue engineering applications. The hybrid sheets have been prepared via co-electrospinning and in-situ crosslinking methods without corrosive solvents and toxic crosslinking agents. To evaluate the proposed scaffold as a controlled release system, the...
In-situ crosslinking of electrospun gelatin-carbodiimide nanofibers: fabrication, characterization, and modeling of solution parameters
, Article Chemical Engineering Communications ; Volume 208, Issue 7 , 2021 , Pages 976-992 ; 00986445 (ISSN) ; Alemzadeh, I ; Vossoughi, M ; Shamloo, A ; Sharif University of Technology
Taylor and Francis Ltd
2021
Abstract
This work has focused on in-situ crosslinking of gelatin (G) to produce electrospun scaffold with improved fiber morphology retention and mechanical properties. As per this approach, we prepared G nanofibers through mixing G, 1-ethyl-3-(3 dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) in the new solvent system. Response surface methodology (RSM) was employed to study the influence of solution parameters on fiber diameter. The morphological structure was examined, and the appropriate level of setting to obtain smooth fibers with a favorable diameter was reported. Results revealed using EDC/NHS for in-situ crosslinking improves the mechanical properties...
Fabrication of a novel 3D scaffold for cartilage tissue repair: In-vitro and in-vivo study
, Article Materials Science and Engineering C ; Volume 128 , 2021 ; 09284931 (ISSN) ; Shamloo, A ; Sharif University of Technology
Elsevier Ltd
2021
Abstract
Self-repairing is not an advanced ability of articular cartilage. Tissue engineering has provided a novel way for reconstructing cartilage using natural polymers because of their biocompatibility and bio-functionality. The purpose of cartilage tissue engineering is to design a scaffold with proper pore structure and similar biological and mechanical properties to the native tissue. In this study, porous scaffolds prepared from gelatin, chitosan and silk fibroin were blended with varying ratios. Between the blends of chitosan (C), gelatin (G) and silk fibroin (S), the scaffold with the weight per volume ratio of 2:2:3 (w/v) showed the most favorable and higher certain properties than the...
The effect of pore morphology and agarose coating on mechanical properties of tricalcium phosphate scaffolds
, Article International Journal of Applied Ceramic Technology ; Volume 19, Issue 5 , 2022 , Pages 2713-2722 ; 1546542X (ISSN) ; Bagheri, R ; Amirkhani, S ; Sharif University of Technology
John Wiley and Sons Inc
2022
Abstract
Three-dimensional biocompatible porous structures can be fabricated using different methods. However, the biological and mechanical behaviors of scaffolds are the center of focus in bone tissue engineering. In this study, tricalcium phosphate scaffolds with similar porosity contents but different pore morphologies were fabricated using two different techniques, namely, the replica method and the pore-forming agent method. The samples fabricated using the pore-forming agent showed more than two times higher compressive and bending strengths and more than three times higher compressive moduli. Furthermore, a thin layer of agarose coating improved the compressive and bending strength of both...
Evaluation of cellular attachment and proliferation on different surface charged functional cellulose electrospun nanofibers
, Article Carbohydrate Polymers ; Volume 207 , 2019 , Pages 796-805 ; 01448617 (ISSN) ; Karimi, A ; Gandomi Ravandi, S ; Vossoughi, M ; Khafaji, M ; Joghataei, M. T ; Faghihi, F ; Sharif University of Technology
Elsevier Ltd
2019
Abstract
Fabrication and characterization of different surface charged cellulose electrospun scaffolds including cellulose acetate (CA), cellulose, carboxymethyl cellulose (CMC) and quaternary ammonium cationic cellulose (QACC) for biomedical applications have been reported in this research. Several instrumental techniques were employed to characterize the nanofibers. MTT assay and cell attachment studies were also carried out to determine the cytocompatibility, viability and proliferation of the scaffolds. Fabricated CA, cellulose, CMC and QACC nanofibers had 100–600 nm diameter, −9, −1.75, −12.8, + 22 mV surface potential, 2.5, 4.2, 7.2, 7 MPa tensile strength, 122, 320, 515, 482 MPa Young modules,...
Pore control in SMA NiTi scaffolds via space holder usage
, Article Materials Science and Engineering C ; Volume 32, Issue 5 , 2012 , Pages 1266-1270 ; 09284931 (ISSN) ; Hosseini, S. R ; Sadrnezhaad, S. K ; Sharif University of Technology
2012
Abstract
Porous NiTi shape memory alloy (SMA) was fabricated by sintering of compressed constituent elements pre-mixed with NaCl or urea spacer holders. Effect of spacer to metal volume-ratio (r S) on shape, size, distribution and openness of the voids was probed by optical metallography, X-ray diffraction (XRD) and scanning electron microscopy (SEM). Differential scanning calorimetry (DSC) was used to determine the SMA transformation temperatures. Controllable void geometry helping osteoblast proliferation and bone cell growth was gained by addition of the spacers. At r S = 0.7, percentage of the open pores reached 52% while at r S = 1.43, interconnected pores with 200 to 500 μm diameter were...
A cellular cardiac matrix-based porous microcarrier as a cell delivery system in myocardial tissue engineering application
, Article Iranian Polymer Journal (English Edition) ; Volume 31, Issue 9 , 2022 , Pages 1079-1091 ; 10261265 (ISSN) ; Mashayekhan, S ; Khanmohammadi, M ; Sharif University of Technology
Springer Science and Business Media Deutschland GmbH
2022
Abstract
Myocardial infarction (MI) causes a high mortality rate in the world every year. Myocardial tissue engineering using extracellular matrix-derived substrate and cytocompatible biopolymers is a promising approach for treating MI. Besides, injectable porous microspheres are developing engineer constructs to use as dual-purpose microcarriers for cell culture and injectable scaffolds in trivial invasiveness for tissue implantation. This study aimed to fabricate porous microcarriers composed of myocardial extracellular matrix and chitosan using an electrospraying technique. The effect of electrospraying parameters, including extracellular matrix/chitosan ratio and voltage, on MCs diameter was...
The impact of zirconium oxide nanoparticles content on alginate dialdehyde-gelatin scaffolds in cartilage tissue engineering
, Article Journal of Molecular Liquids ; Volume 335 , 2021 ; 01677322 (ISSN) ; Salavati Niasari, M ; Mohandes, F ; Firouzi, Z ; Mousavi, S.-D ; Sharif University of Technology
Elsevier B.V
2021
Abstract
The desire to regenerate and repair native tissues can be immediately performed by multiple tissue engineering procedures. Gelatin and alginate are biocompatible and biodegradable polymers. The addition of ZrO2 nanoparticles (NPs) into the alginate-gelatin hydrogel is considered to improve mechanical and chemical properties. Therefore, nanocomposite hydrogels have been manufactured by the freeze-drying procedure utilizing oxidized alginate-gelatin with ZrO2 NPs as a reinforcement. The fabricated nanocomposite hydrogels were character-ized by FTIR, FESEM, and rheometer. The hydrogels containing a higher ZrO2 NPs content (1.5%) have better mechanical properties than the hydrogels without NPs....
Mechanical reinforcement of urinary bladder matrix by electrospun polycaprolactone nanofibers
, Article Scientia Iranica ; Volume 24, Issue 6 , 2017 , Pages 3476-3480 ; 10263098 (ISSN) ; Rajabi Zeleti, S ; Naji, M ; Ghanian, M. H ; Baharvand, H ; Sharif University of Technology
Abstract
For a successful repair and reconstruction of bladder tissue, fabrication of scaffolds with proper biochemical and biomechanical characteristics is necessary. Decellularized bladder tissue has been proposed in previous studies as a gold standard material for scaffold fabrication. However, weak mechanical properties of such a load-bearing tissue has remained a challenge. Incorporation of both biological and synthetic materials has been known as an effective strategy for improving mechanical and biological properties of the scaffolds. In the present work, a simple process was developed to fabricate hybrid hydrogel scaffolds with a biomimetic architecture from the natural urinary bladder...
ZnO-incorporated polyvinylidene fluoride/poly(ε-caprolactone) nanocomposite scaffold with controlled release of dexamethasone for bone tissue engineering
, Article Applied Physics A: Materials Science and Processing ; Volume 128, Issue 8 , 2022 ; 09478396 (ISSN) ; Mohammadi, M ; Mashayekhan, S ; Sharif University of Technology
Springer Science and Business Media Deutschland GmbH
2022
Abstract
Here we report on the development of a hybrid nanofibrous scaffold made from polyvinylidene fluoride (PVDF) nanofibers embedding zinc oxide nanorods (ZnOns), and poly(ε-caprolactone) (PCL) nanofibers incorporating dexamethasone (DEX)-loaded chitosan nanoparticles using dual-electrospinning method. Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and tensile analysis were carried out for physiochemical characterization of the scaffolds, followed by DEX release profile. In addition, an MTT assay was conducted to assess the viability of mouse bone marrow-derived mesenchymal stem cells (mBMSCs) on the hybrid nanofibrous scaffold....
Biological evaluation of a novel tissue engineering scaffold of Layered Double Hydroxides (LDHs)
, Article Key Engineering Materials, 6 November 2011 through 9 November 2011 ; Volume 493-494 , November , 2012 , Pages 902-908 ; 10139826 (ISSN) ; 9783037852552 (ISBN) ; Solati Hashjin, M ; Shokrgozar, M. A ; Bonakdar, S ; Ganji, Y ; Mirjordavi, N ; Ghavimi, S. A ; Khashayar, P ; Sharif University of Technology
2012
Abstract
Bone Tissue Engineering (BTE) composed of three main parts: scaffold, cells and signaling factors. Several materials and composites are suggested as a scaffold for BTE. Biocompatibility is one of the most important property of a BTE scaffold. In this work synthesis of a novel nanocomposite including layered double hydroxides (LDH) and gelatin is carried out and its biological properties were studied. The co-precipitation (pH=11) method was used to prepare the LDH powder, using calcium nitrate, Magesium nitrate and aluminum nitrate salts as starting materials. The resulted precipitates were dried. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron...
Processing and properties of nanofibrous bacterial cellulose-containing polymer composites: a review of recent advances for biomedical applications
, Article Polymer Reviews ; Volume 60, Issue 1 , 2020 , Pages 144-170 ; Mahmoodi, A ; Mahmoudi, N ; Zandi, N ; Simchi, A ; Sharif University of Technology
Taylor and Francis Inc
2020
Abstract
Bacterial cellulose (BC) is an extracellular natural polymer produced by many microorganisms and its properties could be tailored via specific fabrication methods and culture conditions. There is a growing interest in BC derived materials due to the main features of BC such as porous fibrous structure, high crystallinity, impressive physico-mechanical properties, and high water content. However, pristine BC lacks some features, limiting its practical use in varied applications. Thus, fabrication of BC composites has been attempted to overcome these constraints. This review article overviews most recent advance in the development of BC composites and their potential in biomedicine including...
Fabrication of biocompatible titanium scaffolds using space holder technique
, Article Journal of Materials Science: Materials in Medicine ; Volume 23, Issue 10 , 2012 , Pages 2483-2488 ; 09574530 (ISSN) ; Sadrnezhaad, S. K ; Shokrgozar, M. A ; Bonakdar, S ; Sharif University of Technology
Springer
2012
Abstract
Open-pore titanium scaffolds were fabricated by sintering of compressed mixtures of TiH1.924 and urea. Spherical and irregular shaped space holders were used to investigate the effect of pore shape on cellular behavior. After removal of the space holder, the shape of the spacers was replicated to the pores. Average diameter of the pores was in the range of 300-600 lm. SEM images showed that titanium hydride resulted in higher surface roughness and larger micro porosities than pure titanium. In vitro evaluationswere carried out by using MTT assay, measuring alkaline phosphatase activity and alizarin red staining in flow perfusion bioreactor for cell culture. Observations revealed excellent...
Preparation and characterization of a composite biomaterial including starch micro/nano particles loaded chitosan gel
, Article Carbohydrate Polymers ; Volume 174 , 2017 , Pages 633-645 ; 01448617 (ISSN) ; Bagheri, R ; Solouk, A ; Sharif University of Technology
Abstract
Thermosensitive Chitosan hydrogels which can be injected into defects with minimally invasive approach were prepared. Also starch micro/nano particles were synthesized via water-in-oil (W/O) miniemulsion technique. The starch particles were incorporated into the chitosan hydrogel to prepare injectable thermosensitive hydrogel composites. Tube inverting method, compression tests, swelling studies, XRD, SEM, OM, DLS, UV–vis spectroscopy were used for investigations. Results revealed that increasing crosslinker and surfactant contents and stirring rate leads to particle size reduction. Particle size was modeled using design of experiments (DOE) via the response surface method (RSM). Due to...
An alternative mechanism for the formation of high density lipoprotein in peripheral tissue
, Article Scientia Iranica ; Volume 23, Issue 2 , 2016 , Pages 600-608 ; 10263098 (ISSN) ; Saidi, M. S ; Rismanian, M ; Firoozabadi, B ; Amininasab, M ; Sharif University of Technology
Sharif University of Technology
2016
Abstract
High Density Lipoprotein (HDL) is a lipid-protein complex responsible for transporting cholesterol and triglyceride molecules, as these compounds are unable to dissolve in aqueous environments such as a bloodstream. Among the most well-known possible structures, the belt-like structure is the most common shape proposed for this vital bimolecular complex. In this structure, the protein scaffold encompasses the lipid bilayer and a planar circular structure is formed. Several HDL simulations with embedded components in the lipid section were performed. Here, we applied a series of molecular dynamic simulations using the MARTINI coarse grain force field to investigate an HDL model, with pores of...