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Detection of molecular vibrations of atrazine by accumulation of silver nanoparticles on flexible glass fiber as a surface-enhanced Raman plasmonic nanosensor
, Article Optical Materials ; Volume 128 , 2022 ; 09253467 (ISSN) ; Kordzadeh, A ; Zeinalizad, L ; Sahbafar, H ; Aghanouri, H ; Hadi, A ; Ghaderi, S ; Sharif University of Technology
Elsevier B.V
2022
Abstract
Surface-Enhanced Raman Spectroscopy (SERS) is a sensitive vibration spectroscopy method applied to analyze a variety of analytes, including toxins and pesticides. The SERS method is an accurate method for detecting significantly low concentrations of biomaterials and chemicals. In the present study, in order to detect atrazine pesticide, the glass fiber substrates coated with silver nanoparticles have been used as SERS plasmonic nanosensors. First, silver nanoparticles were prepared by applying a chemical approach named the Tollens' method, and the SERS plasmonic substrates (SPS) were fabricated by depositing the colloidal silver solution on a glass fiber substrate. The SERS plasmonic...
Chaperones promote remarkable solubilization of salmonella enterica serovar enteritidis flagellin expressed in escherichia coli
, Article Protein and Peptide Letters ; Volume 27, Issue 3 , 2020 , Pages 210-218 ; Sadeghi, Z ; Tarahomjoo, S ; Yaghmaie, S ; Sharif University of Technology
Bentham Science Publishers
2020
Abstract
Background: Flagellin of Salmonella enterica serovar Enteritidis (SEF) stimulates immune responses to both itself and coapplied antigens. It is therefore used in vaccine development and immunotherapy. Removal of pathogenic S. enterica ser. Enteritidis from SEF production process is advantageous due to the process safety improvement. The protein solubility analysis using SDS-PAGE indicated that 53.49% of SEF expressed in Escherichia coli formed inclusion bodies. However, the protein recovery from inclusion bodies requires a complex process with a low yield. Objective: We thus aim to study possibility of enhancing SEF expression in E. coli in soluble form using chemical and molecular...