Investigating the Effect of Niosomes Containing Simvastatin on Breast Cancer Cell Line

, M.Sc. Thesis Sharif University of Technology Akbarzadeh, Iman (Author) ; Yaghmaei, Soheila (Supervisor) ; Bakhshandeh Abkenar, Haleh (Supervisor) ; Ahangari Cohan, Reza (Supervisor)

Abstract

Breast cancer is the fifth cause of death from cancer and one of the most common fatal cancers in women. The commonly drugs used to treat cancer often destroy healthy tissues and cells and cause common side effects. It is also necessary to consume a large amount of the drug to achieve a particular effect. One of the applications of nanotechnology is drug delivery. Niosomes are one of the newest drug delivery systems that are nowadays much more widely considered due to better penetration and controlled release. One of the most effective medicines for treating breast cancer is statins. These drugs are a group of lipids lowering drugs that lower the synthesis of cholesterol by inhibiting the...

Label-Free real-time detection of HBsAg using a QCM immunosensor

, Article Clinical Laboratory ; Volume 68, Issue 4 , 2022 , Pages 707-720 ; 14336510 (ISSN) Saffari, Z ; Ghavidel, A ; Ahangari Cohan, R ; Hamidi Fard, M ; Khoobi, M ; Aghasadeghi, M ; Norouzian, D ; Sharif University of Technology

Verlag Klinisches Labor GmbH 2022

Abstract

Background: Hepatitis B virus surface antigen (HBsAg) is an important protein in both diagnosis and prevention of hepatitis B infection. In the current study, a piezoelectric immunosensor based on antibody-antigen interaction was designed to detect HBsAg. A quartz crystal microbalance system was employed to detect antibody-antigen interaction. Methods: At first, an oscillator was designed to measure the resonant frequency affected by the reactants using IC 74LVC1GX04. Antibody against HBsAg was immobilized on 10 MHz AT-cut quartz crystal. The surface modifications were monitored by atomic force microscopy (AFM) and contact angle measurements. Different concentrations of antibody were used...

Niosomal delivery of simvastatin to MDA-MB-231 cancer cells

, Article Drug Development and Industrial Pharmacy ; Volume 46, Issue 9 , 2020 , Pages 1535-1549 Akbarzadeh, I ; Saremi Poor, A ; Yaghmaei, S ; Norouzian, D ; Noorbazargan, H ; Saffar, S ; Ahangari Cohan, R ; Bakhshandeh, H ; Sharif University of Technology

Taylor and Francis Ltd 2020

Abstract

Objective: The objective of this study was to use nano-niosomal formulations to deliver simvastatin as a poor-water soluble drug into breast cancer cells. Significance: Our study focused on the problem associated with poor water-soluble drugs which have significant biological activity in vivo. Methods: Different niosomal formulations of simvastatin were prepared and characterized in terms of morphology, size, encapsulation efficiency (EE), and release kinetic. Antiproliferative activity and the mechanism were assessed by quantitative real-time PCR and flow cytometry. Moreover, confocal microscopy was employed to analyze the cell uptake of simvastatin loaded niosomes to the cancerous cells....

Development of a novel nano-sized anti-VEGFA nanobody with enhanced physicochemical and pharmacokinetic properties

, Article Artificial Cells, Nanomedicine and Biotechnology ; Volume 46, Issue 7 , 2018 , Pages 1402-1414 ; 21691401 (ISSN) Khodabakhsh, F ; Norouzian, D ; Vaziri, B ; Ahangari Cohan, R ; Sardari, S ; Mahboudi, F ; Behdani, M ; Mansouri, K ; Mehdizadeh, A ; Sharif University of Technology

2018

Abstract

Since physiological and pathological processes occur at nano-environments, nanotechnology has considered as an efficient tool for designing of next generation specific biomolecules with enhanced pharmacodynamic and pharmacodynamic properties. In the current investigation, by control of the size and hydrodynamic volume at the nanoscale, for the first time, physicochemical and pharmacokinetic properties of an anti-VEGFA nanobody was remarkably improved by attachment of a Proline-Alanine-Serine (PAS) rich sequence. The results elucidated unexpected impressive effects of PAS sequence on physicochemical properties especially on size, hydrodynamics radius, and even solubility of nanobody. CD...

Development of a novel nano-sized anti-VEGFA nanobody with enhanced physicochemical and pharmacokinetic properties

, Article Artificial Cells, Nanomedicine and Biotechnology ; 2017 , Pages 1-13 ; 21691401 (ISSN) Khodabakhsh, F ; Norouzian, D ; Vaziri, B ; Ahangari Cohan, R ; Sardari, S ; Mahboudi, F ; Behdani, M ; Mansouri, K ; Mehdizadeh, A ; Sharif University of Technology

2017

Abstract

Since physiological and pathological processes occur at nano-environments, nanotechnology has considered as an efficient tool for designing of next generation specific biomolecules with enhanced pharmacodynamic and pharmacodynamic properties. In the current investigation, by control of the size and hydrodynamic volume at the nanoscale, for the first time, physicochemical and pharmacokinetic properties of an anti-VEGFA nanobody was remarkably improved by attachment of a Proline-Alanine-Serine (PAS) rich sequence. The results elucidated unexpected impressive effects of PAS sequence on physicochemical properties especially on size, hydrodynamics radius, and even solubility of nanobody. CD...

A quartz crystal microbalance biosensor based on polyethylenimine-modified gold electrode to detect hepatitis B biomarker

, Article Analytical Biochemistry ; Volume 661 , 2023 ; 00032697 (ISSN) Saffari, Z ; Sepahi, M ; Ahangari Cohan, R ; Khoobi, M ; Hamidi Fard, M ; Ghavidel, A ; Aghasadeghi, M. R ; Norouzian, D ; Sharif University of Technology

Academic Press Inc 2023

Abstract

Biomarkers-based QCM-biosensors are suitable tools for the label-free detection of infectious diseases. In the current study, a QCM-biosensor was developed for the detection of HBsAg. Briefly, anti-HBsAg antibodies were covalently bound to the primary amines after PEI and thiolated-PEI surface modifications of gold-electrode. After RSM optimization, the statistical analysis revealed no significant difference between the immobilization yields of modified layers. Therefore, the PEI-modified QCM-biosensor was selected for further analysis. The PEI-surface was evaluated by FESEM, AFM, ATR-FTIR, and CA measurement. The surface hydrophilicity and its roughness were increased after PEI-coating....

Reduction of Development Cycle Time using Integrated die Design Technology

, M.Sc. Thesis Sharif University of Technology Ahangari, Ali (Author) ; Houshmand, Mahmoud (Supervisor)

Abstract

In today’s industrial world production is considered as a competitive weapon. This has caused many companies active in this field to move towards creating competitive advantage to reduce costs of design, construction and increase production. In particular molding as one of the main industries is among the most economical technological resources of each country. About 50 to 70 percent of the price of a production operation is spent of the strip of sheet by pressing; thus the proper design of a strip of sheet has a great impact on the mold design. Today, in most sectors of the industry there is a need for cutting and packaging of products and form molding and glass cutting to fitting the...

Fabrication of a magnetic alginate-silk fibroin hydrogel, containing halloysite nanotubes as a novel nanocomposite for biological and hyperthermia applications

, Article Scientific Reports ; Volume 12, Issue 1 , 2022 ; 20452322 (ISSN) Eivazzadeh Keihan, R ; Sadat, Z ; Aghamirza Moghim Aliabadi, H ; Ganjali, F ; Kashtiaray, A ; Salimi Bani, M ; Komijani, S ; Ahadian, M. M ; salehpour, N ; Ahangari Cohan, R ; Maleki, A ; Sharif University of Technology

Nature Research 2022

Abstract

In this study, the main focus was on designing and synthesizing a novel magnetic nanobiocomposite and its application in hyperthermia cancer treatment. Regarding this aim, sodium alginate (SA) hydrogel with CaCl2 cross-linker formed and modified by silk fibroin (SF) natural polymer and halloysite nanotubes (HNTs), followed by in situ Fe3O4 magnetic nanoparticles preparation. No important differences were detected in red blood cells (RBCs) hemolysis, confirming the high blood compatibility of the treated erythrocytes with this nanobiocomposite. Moreover, the synthesized SA hydrogel/SF/HNTs/Fe3O4 nanobiocomposite does not demonstrate toxicity toward HEK293T normal cell line after 48 and 72 h....

Magnetic carboxymethyl cellulose/silk fibroin hydrogel embedded with halloysite nanotubes as a biocompatible nanobiocomposite with hyperthermia application

, Article Materials Chemistry and Physics ; Volume 287 , 2022 ; 02540584 (ISSN) Eivazzadeh Keihan, R ; Choopani, L ; Aghamirza Moghim Aliabadi, H ; Ganjali, F ; Kashtiaray, A ; Maleki, A ; Ahangari Cohan, R ; Salimi Bani, M ; Komijani, S ; Ahadian, M. M ; Salehpour, N ; Mahdavi, M ; Sharif University of Technology

Elsevier Ltd 2022

Abstract

A novel and high potent magnetic nanobiocomposite was prepared based on Carboxymethyl cellulose (CMC) and epichlorohydrin (ECH) cross-linker interactions to form a three-dimensional cross-linked CMC hydrogel followed by silk fibroin (SF) and halloysite nanotubes (HNTs) modifications and further in situ Fe3O4 magnetic nanoparticles (MNPs) formation. Different analytical techniques like FT-IR, EDX, FE-SEM, XRD, TGA, and VSM were used to characterize the structure of the prepared nanobiocomposite. The spherical morphology of Fe3O4 MNPs and tubular HNTs were presented in the FE-SEM images. In biological tests, since the hemolysis percent was even less than the negative control, the CMC...

Crowdfunding Platform for Academic Research Projects on the Blockchain Platform

, M.Sc. Thesis Sharif University of Technology Ahangari, Mohammad Hossein (Author) ; Habibi, Jafar (Supervisor)

Abstract

The emerging blockchain technology has introduced a new approach in today's industry, creating new opportunities in technical, financial, and economic domains. With the emergence of blockchain-based platforms such as Ethereum and the capability of smart contracts, the possibility of creating decentralized systems has arisen. One of the most popular decentralized systems in the realm of finance is crowdfunding platforms that assist technical and academic projects in need of capital for industrialization. Startups or research-academic projects secure the necessary funding by selling initial offering tokens to both small and large-scale investors. In this research, we aim to present an...

Design and physicochemical characterization of lysozyme loaded niosomal formulations as a new controlled delivery system

, Article Pharmaceutical Chemistry Journal ; Volume 53, Issue 10 , 2020 , Pages 921-930 Sadeghi, S ; Ehsani, P ; Cohan, R. A ; Sardari, S ; Akbarzadeh, I ; Bakhshandeh, H ; Norouzian, D ; Sharif University of Technology

Springer 2020

Abstract

Lysozyme loaded niosomes containing various molar ratios of two kinds of surfactants were prepared and the properties of these niosomal formulations were studied. The results revealed that the size of niosomes varied between 240.06 ± 32.41 and 895.2 ± 20.84 nm. Formulations with the lowest size and no precipitation had entrapment efficiencies ranging from 60.644 ± 3.310 to 66.333 ± 1.98%. Their controlled release profiles after 48 h were 15.67, 20.67 and 31.50%. After 2 months, the most stable formulation in terms of size, PDI, zeta potential, and entrapment efficiency was used to study the secondary structures of lysozyme in niosomal and free forms. Lysozyme loaded niosome and lysozyme...

New proline, alanine, serine repeat sequence for pharmacokinetic enhancement of anti-vegf single-domain antibody

, Article Journal of Pharmacology and Experimental Therapeutics ; Volume 375, Issue 1 , July , 2020 , Pages 69-75 Khodabakhsh, F ; Salimian, M ; Mehdizadeh, A ; Khosravy, M. S ; Vafabakhsh, A ; Karami, E ; Cohan, R. A ; Sharif University of Technology

American Society for Pharmacology and Experimental Therapy 2020

Abstract

Therapeutic fragmented antibodies show a poor pharmacokinetic profile that leads to frequent high-dose administration. In the current study, for the first time, a novel proline, alanine, serine (PAS) repeat sequence called PAS#208 was designed to extend the plasma half-life of a nanosized anti-vascular endothelial growth factor-A single-domain antibody. Polyacrylamide gel electrophoresis, circular dichroism, dynamic light scattering, and electrophoretic light scattering were used to assess the physicochemical properties of the newly designed PAS sequence. The effect of PAS#208 on the biologic activity of a single-domain antibody was studied using an in vitro proliferation assay. The...

Design of experiment, preparation, and in vitro biological assessment of human amniotic membrane extract loaded nanoparticles

, Article Current Pharmaceutical Biotechnology ; Volume 21, Issue 3 , 2020 , Pages 256-267 Shabani, A ; Atyabi, F ; Khoshayand, M. R ; Mahbod, R ; Cohan, R. A ; Akbarzadeh, I ; Bakhshandeh, H ; Sharif University of Technology

Bentham Science Publishers 2020

Abstract

Background: Human amniotic membrane grafting could be potentially useful in ocular surface complications due to tissue similarity and the presence of factors that reduce inflammation, vascu-larization, and scarring. However, considerations like donor-derived infectious risk and the requirement of an invasive surgery limit the clinical application of such treatments. Moreover, the quick depletion of bioactive factors after grafting reduces the efficacy of treatments. Therefore, in the current study, the possibility of nano delivery of the bioactive factors extracted from the human amniotic membrane to the ocular surface was investigated. Materials and Methods: Nanoparticles were prepared...

A misbehavior‐tolerant multipath routing protocol for wireless Ad hoc networks [electronic resource]

, Article International Journal of Research in Wireless Systems (IJRWS) ; Vol. 2, Issue 9, pp. , Sep. 2013 Sedghi, H. (Haniyeh) ; Pakravan, Mohammad Reza ; Aref, Mohammad Reza ; Sharif University of Technology

Abstract

Secure routing is a major key to service maintenance in ad hoc networks. Ad hoc nature exposes the network to several types of node misbehavior or attacks. As a result of the resource limitations in such networks nodes may have a tendency to behave selfishly. Selfish behavior can have drastic impacts on network performance. We have proposed a Misbehavior-Tolerant Multipath Routing protocol (MTMR) which detects and punishes all types of misbehavior such as selfish behavior, wormhole, sinkhole and grey-hole attacks. The protocol utilizes a proactive approach to enforce cooperation. In addition, it uses a novel data redirection method to mitigate the impact of node misbehavior on network...

Analysis and data-based reconstruction of complex nonlinear dynamical systems : using the methods of stochastic processes

, Book Rahimi Tabar, M. Reza

Springer International Publishing 2019

Abstract

This book focuses on a central question in the field of complex systems: Given a fluctuating (in time or space), uni- or multi-variant sequentially measured set of experimental data (even noisy data), how should one analyse non-parametrically the data, assess underlying trends, uncover characteristics of the fluctuations (including diffusion and jump contributions), and construct a stochastic evolution equation?
Here, the term "non-parametrically" exemplifies that all the functions and parameters of the constructed stochastic evolution equation can be determined directly from the measured data.
The book provides an overview of methods that have been developed for the analysis of...

Fabrication and Study of Mechanical Behavior of in Situ Microfibrillar- Reinforced Composites of Polypropylene/Recycled Poly (Ethylene Terephthalate)Toughened with Rubber Particles

, M.Sc. Thesis Sharif University of Technology Motahari, Tayebeh (Author) ; Bagheri, Reza (Supervisor) ; Alizadeh, Reza (Supervisor)

Abstract

The use of polymers is increasing day by day due to low density, reasonable price and ability to produce different products. On the other hand, the accumulation of polymer wastes in nature is one of the environmental concerns in today's world, which is mainly due to the widespread use of polymers in the packaging industry and disposable applications. In order to solve this problem, recycling is recommended as the most appropriate and economical solution. Because in addition to consuming polymer waste, it also saves energy and reduces carbon footprint.Polyethylene terephthalate (PET) is one of the polymeric materials which; It has a special place in the packaging industry and is widely used...

Characterization of Mechanical Properties of Polymer Nanocomposites with Spherical Inhomogeneities

, M.Sc. Thesis Sharif University of Technology Goudarzi, Taha (Author) ; Naghdabadi, Reza (Supervisor) ; Bagheri, Reza (Supervisor)

Abstract

The improved properties of nanocomposites are not achievable with conventional composites. Scale effect is one the most important parameters in the physical and mechanical properties of polymeric nanocomposites. One of the physical phenomena, which can be related to the scale effect, is the very large interface between the nanoparticles and the polymeric matrices. Motional behavior and conformation of polymeric chains change near the nanoparticles surfaces. Due to high interface of the nanoparticles with the polymeric matrices the amount of these types of changes in the polymeric chains are so large that can change the physical and mechanical properties of polymeric nanocomposites. In this...

On small uniquely vertex-colourable graphs and Xu's conjecture [electronic resource]

, Article Discrete Mathematics ; Volume 223, Issues 1–3, 28 August 2000, Pages 93–108 Daneshgar, A. (Amir) ; Naserasr, Reza ; Sharif University of Technology

Abstract

Consider the parameter Λ(G) = |E(G)| - |V(G)|(k - 1) + (k2) for a k-chromatic graph G, on the set of vertices V(G) and with the set of edges E(G). It is known that Λ(G)≥0 for any k-chromatic uniquely vertex-colourable graph G (k-UCG), and, S.J. Xu has conjectured that for any k-UCG, G, Λ(G) = 0 implies that cl(G) = k; in which cl(G) is the clique number of G. In this paper, first, we introduce the concept of the core of a k-UCG as an induced subgraph without any colour-class of size one, and without any vertex of degree k - 1. Considering (k, n)-cores as k-UCGs on n vertices, we show that edge-minimal (k, 2k)-cores do not exist when k ≥ 3, which shows that for any edge-minimal k-UCG on 2k...

Molecular Dynamics Simulation of Nano-Diamond Synthesis by Shock Wave

, Ph.D. Dissertation Sharif University of Technology Mahnama, Maryam (Author) ; Naghdabadi, Reza (Supervisor) ; Movahhedy, Mohammad Reza (Co-Advisor)

Abstract

In the field of high-pressure material science, diverse carbon systems under pressure have been intensively studied with interest in synthesizing new phases. A variety of these synthetic phases which have met various applications in today technology are called the amorphous diamond. The pressure-induced structural transition of carbonaceous material to amorphous diamond is realized by shock compression and rapid quenching. The shock compression and rapid quenching generate the high pressure (several GPa) and the temperature (several hundred K) in a fraction of a microsecond.Since the mechanical and electrical properties of the synthetic diamond are severely sensitive to the atomic structure,...

An Experimental Study of the Effects of Sweep Wing on the Boundary Layer of 2D Wing

, M.Sc. Thesis Sharif University of Technology Tabrizian, Arshia (Author) ; Soltani, Mohammad Reza (Supervisor) ; Davari, Ali Reza (Co-Advisor)

Abstract

The behavior of boundary layer under the effect of the sweep angle is considered. The measurements were performed by a pitot tube rake. Three models with various sweep angels at angles of attack -2, 0 and 2 degree were tested. Both tip and root of all wings were closed by flat plates in order to prevent the flow to roll-up. However the flow field on the wing was still three-dimensional because of the sweep angle. The velocity on the wing has two components; longitudinal and lateral. The cross flow emerges due to the non-equilibrium of pressure and centrifugal forces. The velocity profiles showed that the magnitude of cross flow was stronger near the leading edge of the wing. The cross flow...