Sharif Digital Repository

Enzymes are an important part of detergents, they reduce the activation energy of the reaction and thus increase the efficiency of the process. Protease enzyme is one of the most common enzymes in detergents. Bacillus strain is the most important strain in the production of this enzyme and among the species of Bacillus, Bacillus subtilis is the most used. Bacillus subtilis is a gram-positive, rod-shaped bacterium. This bacterium is considered a non-pathogenic cell that has the ability to form spores and prevent death and damage in harsh conditions. In this study, CRISPR system was used to modify the genome of Bacillus subtilis ATCC 6633 in order to increase alkaline protease. To increase the... 

Genetic engineering of cells has been one of the most effective ways to increase protein production in wild strains. Among the various genetic engineering techniques, the CRISPR/Cas9 system that directly edits the cell chromosome, yielding greater efficiency and easier operation. In this study, we used this system to modify the genome of Bacillus subtilis ATCC 6633 to increase the production of extracellular alkaline protease enzyme. Alkaline protease enzyme has many applications in different industries, but in this study, the application of this enzyme in the detergent industry has been considered. To increase the production of this enzyme, we disrupted and prevented the gene responsible... 

The frequency divider is one of the components of a frequency synthesizer. Regenerative frequency divider is one of the frequency division methods and is used in narrow band applications. In this thesis, the process of designing & fabricating Ka band and Ku band regenerative frequency dividers is reported. A bandwidth of 550MHz for the Ka band divider and 400MHz for the Ku band divider have been achieved. Multiple passive structures such as microstrip filters, Wilkinson power dividers and a waveguide-to-microstrip transition have been designed during the fabrication of the mentioned frequency dividers. According to the results of the simulations, the bandwidth of the waveguide-to-microstrip... 

Industrial enzymes can generally be classified into three groups: carbohydrases, proteases and lipases, which account for a major part of the global enzyme trade. Proteases, in particular, have always been extensively studied and investigated due to their widespread use in various industries, such as food, pharmaceutical, chemical, and agricultural sectors. Proteases are a group of proteolytic enzymes that break down proteins and peptides into their constituent amino acids by hydrolyzing peptide bonds. Among the family of proteases, alkaline serine proteases have special biocatalytic abilities in both aqueous and organic environments. Their suitable resistance in alkaline environments and... 

In this thesis first of all we investigate phase noise and it's generation factors. Then we design and implement an ultra low phase noise oscillator. To do this, an ultra low phase noise oscillator which is tunable in 1-2GHz with 100MHz steps will be designed. The outline of the circuit is as follows: at the first we design a VCO which is ultra low phase noise and mechanically tunable in 1-2GHz by means of rotation of a handle. Then a phase locked loop will be built with the help of an ultra low phase noise OCXO at 100MHz and one SPD1 which generates harmonics of OCXO's output frequency. For the next, design and implementation of a 1.6GHz oscillator with fixed output frequency has been done.... 

Today, by emerging wearable technologies and digital health, it is possible to constantly monitor vital signals of people without time-consuming and costly referrals to medical centers. Applications of wearable sensors for health monitoring include electrocardiograms (ECG), blood pressure measurement, and blood sugar measurement, etc. One of the important fields of health monitoring is cardiac arrhythmia detection. Cardiac arrhythmia is any kind of anomalies in heart beat rhythm that can lead to serious damage to vital organs of the body. It is estimated that between 750 thousand to 1.5 million people are facing cardiac arrhythmia in Iran. Therefore, early detection of cardiac arrhythmia is... 

Since the early 90s, nanotechnology has contributed to several developments in a wide range of Science and industry such as medicine, Physics, Chemistry, Aerospace, electronics and Biology. In recent years, nanotechnology has also made development in the oil and gas industry. The drilling fluid is already one of the most important parts in drilling operation.The physical properties of a mud, rheological properties and minimize fluid invasion into the formation with perfect mudcake are important to assist in optimizing results. Nano-materials as one of the achievements of recent years are effective in achieving these purposes. To obtain the above properties, a variety of polymers as... 

One of the main geotechnical phenomena that causes severe damage during earthquake is liquefaction. This incident occurs due to the undrained behavior of loose sandy soils. In this occurrence, the interaction between the solid and liquid phases in a cyclic motion leads to an increase in pore water pressure and a decrease in interactive forces (effective forces) between grains in the solid grains. Therefore, shear strength decreases. Different aspects of the aforementioned phenomenon is investigated, namely, field, laboratory, and numerical; nevertheless, the study is largely focused on the numerical modelling. Most studies in this field are carried out on a one-layer saturated sand. However,... 

Reverse osmosis (RO) is by far the most widely used membrane desalination process worldwide. The RO process depends on the properties of semi-permeable membranes which allow the separation of water from a salt solution by applying external pressure higher than the osmotic pressure on the seawater. The main problem in membrane separation process is the fouling of the membrane walls, which decreases the permeate flux. Having spacer filaments in the feed channel has been proven to dynamically change the concentration boundary layer and preventing fouling. The impact of spacer configurations (cavity, zigzag and submerged) on the alleviation of concentration polarization and the enhancement of... 

Because of the importance of gas-solid fluidized beds in industrial plants, many efforts have been done for predicting their behavior up to now. Generally, there are two major attitudes for describing and simulating the hydrodynamics of fluidized beds, Eulerian-Eulerian methods and Eulerian-Lagrangian metods. Both of these approaches have their own advantages and disadvantages and a lot of studies have been done to understand the nature of gas-solid fluidized beds with aid of these models. Due to fewer presumptions in the basic structure of Eulerian-Lagrangian methods such as DEM in comparison with Eulerian-Eulerian methods like TFM, these models can predict the hydrodynamics of fluidized... 

In this study the behavior of steel bracings under cyclic loading, is modeled with finite element method. Analysis have shown that after local buckling in plastic mid-span position, due to the severe geometry change, the strain grows rapidly, The sharp growth of plastic strains is entitled as strain rises. Geometry change created will cause the collapse brace speeding up. In fact, the main local buckling can be used as a determinative factor in performance base design for steel braces. So the thesis has been investigated, using the parameters fracture life to quantify local buckling and fracture. Also with using the CVGM model, the fracture of the brace could be predicted. The analytical... 

The title of this thesis is cosmological perturbation theory in brane cosmology. In this thesis, we investigate brane cosmology, specifically we will mainly investigate super-horizon curvature perturbation during inflation, radiation-dominated and matter-dominated eras. The areas to be reviewed and the steps necessary to reach the final conclusion of this thesis are as follows: Firstly, we will have an overview of brane cosmology. Then, we will investigate the inflation within the brane cosmology framework. Furthermore, by investigating the cosmological perturbation theory during inflation, radiation-dominated and matter-dominated eras, we investigate the evolution of curvature perturbation... 

Statistical studies indicate the fact that in Iran, like many other developing countries, a significant percentage of buildings are made of traditional materials and most of them are adobe buildings especially in rural areas. Furthermore, geological data indicates that Iran is located in alps-Himalayas seismic zone and exposed by destructive earthquakes and adobe buildings suffer the most damages comparing with other type of the structures. According to these explanations, this research deals with adobe buildings behavior and provides solutions for reinforcing them and improving the seismic response of these buildings. These studies include numerical modeling with finite elements methods by... 

Computational grids enabling resource sharing and coordination are now one of the common and acceptable technologies used for solving computational intensive applications rising in scientific and industrial problems. Nevertheless, due to the heterogeneity, dynamicity and autonomy of the grid resources, task scheduling within these systems has become a challenging research area. Applying the market model to the grids is a good approach which can easily take the dynamic characteristics of the grid resources into account and simplify the scheduling problem considering user-centric factors.In this thesis a two level market-based model for task scheduling problem in grid environment is presented.... 

In this thesis we consider the problem of distributed computation by many processors.We mainly concentrate on matrix multiplication problem in this thesis because of its importance. A distributed system consists of N worker processors and one master processor. The master processor should distribute the computation between workers and after computation in each of them, collect the results. In this thesis, we are going to mitigate the effect of straggler processors by using coding methods. Straggler processors can cause delays in the computation time.In this thesis, we firstly introduce a method to multiply any number of matrices in each other. The proposed method occurred in one shot without... 

A social robot is an artificial intelligence-based system designed for humans and other robots. These robots can be used in various settings. The more closely a robot resembles and behaves like a human, the more attention and popularity it will gain. The aim of this research is to humanize the behavior patterns of the robot, especially its gaze in different social situations. The social situations considered in this study are common in human life. In these situations, the robot should make decisions based on the activities that humans are engaged in. The research consists of three parts that design neural networks using LSTM and Transformers architecture. The first part utilizes data...