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The production of protease by Bacillus licheniformis BBRC 100053 was studied. The most appropriate medium for the growth and protease production is composed of: lactose 1 %, yeast extract 0.5 %, peptone 0.5 %, KH 2PO4 0.1%, MgSO4.7H2O 0.02 %. Enzyme production corresponded with growth and reached a maximums level (589 U/ml) during the stationary phase at 35 °C, pH equivalent to 10 and with 150 rpm after 73 hours. Protease activity was highest at pH 8 and 45 °C. The best carbon sources are respectively lactose and maltose and the best nitrogen source is peptone The protease was highly active and stable from pH 7.0 to 11.0 with an optimum at pH 7-8.Thermo stability of the enzyme was considered... 

The production of protease by Bacillus licheniformis BBRC 100053 was studied. The most appropriate medium for the growth and protease production is composed of: lactose 1%, yeast extract 0.5%, peptone 0.5%, KH2PO 4 0.1%, MgSO4.7H2O 0.02%. Enzyme production corresponded with growth and reached a maximums level (589 U/ml) during the stationary phase at 35°C, pH equivalent to 10 and with 150 rpm after 73 hours. Protease activity was highest at pH 8 and 45°C. The best carbon sources are respectively lactose and maltose and the best nitrogen source is peptone. The protease was highly active and stable from pH 7.0 to 11.0 with an optimum at pH 7-8.... 

Background: It is now possible to analyze cellular heterogeneity at the single-cell level thanks to the rapid developments in single-cell sequencing technologies. The clustering of cells is a fundamental and common step in heterogeneity analysis. Even so, accurate cell clustering remains a challenge due to the high levels of noise, the high dimensions, and the high sparsity of data. Results: Here, we present SCEA, a clustering approach for scRNA-seq data. Using two consecutive units, an encoder based on MLP and a graph attention auto-encoder, to obtain cell embedding and gene embedding, SCEA can simultaneously achieve cell low-dimensional representation and clustering performing various... 

This paper attempts to develop the optimal solution for an inventoryproblem consisting of a single machine which produces items some of which may bedefective. We assume that no shortages are allowed and all defective items areto be reworked. Setup cost for rework and waiting time of defectives areconsidered. We consider two different policies where in the first policy in eachcycle after the regular production the machine is setup for the rework ofdefectives of the same cycle. In the second policy several cycles constitute aperiod. All the defectives produced in the period are reworked in the last cycleof that period. At the end of the rework cycle the whole process starts allover again. One... 

This paper deals with a two-echelon inventory system consisting of onesupplier and N retailers. Each retailer faces an independent Poisson demand withthe same rate and applies a new ordering policy called one-for-one- periodordering policy for its inventory control. In this ordering policy the ordersize is equal to one and the time interval between any two consecutive ordersforms a common fixed cycle. Thus, the supplier faces a deterministic demand andadopts a deterministic inventory policy. At each cycle he orders a batch of sizeN to his own supplier. Upon receipt of each batch he sends 1 unit of theproduct to each retailer with a transportation cost. In this paper, for theabove system we...