Development of injectable hydrogels based on human amniotic membrane and polyethyleneglycol-modified nanosilicates for tissue engineering applications

, Article European Polymer Journal ; Volume 179 , 2022 ; 00143057 (ISSN) Kafili, G ; Tamjid, E ; Niknejad, H ; Simchi, A ; Sharif University of Technology

Elsevier Ltd 2022

Abstract

Recently, decellularized amniotic membrane-derived hydrogels (DAMHs) have received significant attention for wound care, ocular surface reconstruction, and chondral healing. Despite the advantages of DAMHs for tissue engineering (TE), the loss of structural components during the decellularization process mitigates their mechanical strength and thus limits their practical application. Herein, we present a method for the surface modification of two-dimensional nanosilicates (laponite) as a rheological modifier to tailor the properties of DAMHs. Results show that after introducing nanosilicates, severe aggregation of the nanoparticles occurs, owing to the shielding effect of ions on the surface...

Development of printable nanoengineered composite hydrogels based on human amniotic membrane for wound healing application

, Article Journal of Materials Science ; Volume 58, Issue 30 , 2023 , Pages 12351-12372 ; 00222461 (ISSN) Kafili, G ; Tamjid, E ; Niknejad, H ; Simchi, A ; Sharif University of Technology

Springer 2023

Abstract

Recently, decellularized amniotic membranes (dAM) have attracted significant interest as a valuable source for the development of shear-thinning hydrogels and bioinks. However, the inferior rheological behavior and weak mechanical durability restrict the printability of the hydrogels and their stability after three-dimensional (3D) bioprinting. Therefore, a chemical or physical modification with biocompatible components is necessary to improve dAM-derived hydrogels’ properties. The present study proposes a strategy to fabricate printable dAM-derived hydrogels (DAMHs) supplemented with sodium alginate and Laponite nanoplatelets. Rheological experiments determined the key role of Laponite...

3D Bioprinting of Amniotic Membrane-Based Nanocomposite for Tissue Engineering Applications: Evaluation of Rheological, Mechanical and Biological Properties

, Ph.D. Dissertation Sharif University of Technology Kafili, Golara (Author) ; Simchi, Abdolreza (Supervisor) ; Tamjid, Elnaz (Supervisor) ; Niknejad, Hassan (Co-Supervisor)

Abstract

3D bioprinting is an additive manufacturing method that facilitates the deposition of the desired cells and biomaterials at any pre-defined location. This technique also enables control over the internal structure and external dimensions of printed constructs. Among various biomaterials used as bioinks, the bioinks derived from decellularized extracellular matrixes (dECMs) have attracted significant attention due to their bioactivity and being a rich source of biochemical cues. Here in this study, the decellularized amnion membrane (dAM) has been selected as the main component of the bioink formulation because of its biocompatibility, low immunogenicity, antibacterial property, abundance,...