Sharif Digital Repository

The purpose of this reaserch is to create a context in production of Bone Morphogenetic Protein-2 with more effective properties in Bone healing in Iran. Because of demand Of this protein in medical field it could cause an evalution in bone healing. The first section of this reaserch was the Construction of new recombinant plasmid carrying bone morphogenetic protein gene that was title of this thesis. First total RNA was extracted from ostosarcoma cancer cell line MG-63 followed by cDNA synthesis.For amplifying of the mature region of protein a pair of primer with tow restriction site was designed. After Success in PCR and obtaining PCR product with size of 343bp it transferd to vector... 

One of the most important methods of controlling discharge in water industry which is widely used in irrigation, water distribution, waste-water engineering, drainage, flood control and other water related projects, is side weir. The hydraulic behavior of flow over side weir is spatially varied flow with decreasing discharge. Various investigators have related the weir discharge coefficient as a function of main channel upstream Froude number and the fraction of weir height to upstream depth . Performance of sharp crested side weirs with extended length (as labyrinth side weir with asymmetric geometry) has been studied in this thesis. The variables for this study are height of weir ,... 

Air enterance in to flowing water is something that is commonly seen in the water conveyance systems. The mixture of Air and water that flow together create a kind of flow that is called two phase flow. This kind of flow is often seen in culverts and water conveyance systems. There are different flow patterns in two phase flow. Considering pressure fluctuation, pressure drop and pressure recovery, slug flow pattern is the most complicated pattern among all.
Sometimes a singularity is used in a tunnel; one of the singularities that are frequently used in water conveyance system is expansion transition. In this study an experimental setup is used to model two phase slug flow, which passes... 

Local scouring at the downstream of sluice gates in the erosive beds is one of the main concerns of hydraulic engineers because of the significant damage that it can cause to the hydraulic structures. It is important to control the local scour depth at downstream of hydraulic structures to ensure safety operation of these systems. Many researchers have conducted various studies to predict the maximum depth and diameter of scour hole and develop new methods in order to control this phenomenon. One way to overcome this problem is the use of apron, or non-erosive bed. In order to shorten the apron length or decrease the effect of the... 

It is well known that arrays of antennas make it possible to steer the radiation pattern without steering a physical structure. For the past few decades this has been considered for many civil and military communication systems. Recently, ultra-short pulses are considered for high resolution radars and ultra-wideband communication systems. Systems with short pulses and large antenna arrays may benefit from high resolution in both temporal and spatial domains. But the implementation of the ultra-wideband feeding circuits for such antenna arrays is a hard job even with state of the art electronics. Optical technology has been applied, successfully, to a number of radio applications such as... 

Sharp crested weirs are one of the main and oldest means of measuring discharge in water conveyance systems. Many studies have been done on sharp rectangular weirs and have provided many relationships regarding the effect of geometric parameters and various factors on discharge coefficient of the weirs.In some places, due to existence of obstacles or barriers in the channel, it is impossible to create a symmetrical continuous weir some other cases,multiple or asymmetric weirs should be used, which is the main concept of this project.Since it seems unlikely that a purely mathematical model can review the impact of the multiplicity and asymmetry of these weirs without using empirical... 

High-power (108 – 109 W) RF pulses with a carrier frequency of C band and a duration of several oscillation periods can be produced through direct energy conversion of a travelling high-voltage pulse with the use of nonlinear elements.
Nonlinear transmission lines consist either of lumped nonlinear elements and provide spatial dispersion of excited oscillations or of elements uniformly distributed on a certain line segment. The first type of NLTL’s can be referred to NLTL’s with spatial dispersion. The second type of NLTL’s is ensemble of nonlinear oscillators with a characteristic natural frequency and temporal dispersion. In the first case, the duration of electromagnetic pulses is... 

During 1980s Additive Manufacturing technologies for the purpose of prototyping with fast and less expensive, under the name of Rapid Prototyping was invented. But nowadays under the vast progress made in this field, it possesses a notable place in different industries such as automobile manufacturing, aerospace, architecture, medicine and so on for part manufacturing. In all AM machines, the CAD model design part is manufactured by adding layered to each other. In this research, at first by explaining manufacturing process and current different technologies, the place of this manufacturing process in today and future industry, is analyzed. For discussing replacement of this method instead... 

If the size of the conventional telecommunication antennas -that are widely used in transmitting broadcasted radio and TV messages- is reduced by a nanometer size these antennas can be used at optical frequencies. Dimensions of these optical nanoantennas -that made of nanoparticles with highly permittivity- can be smaller than half of the wavelength. Based on this capability, optical nanoantennas have some important applications such as optical communication, photovoltaic devices, non-classical light, and optical sensing. After studying on optical nanoantennas and their features, in this project, we present distinctions between optical NAs and MW antennas and then investigate about different... 

The Deep Fading Effect is a destructive factor in communication systems, one of the solutions to eliminate it is the use of Diversity, and there are two types of indicators of Diversity, Spatial Diversity and Polarization Diversity. Spatial Diversity is a common method to reduce the Fading Effect while increasing the cost and increasing the volume of the communication station, which to optimize the cost and reduce the volume of the communication station, the Polarization Diversity Structure is added to the telecommunication system. To apply Spatial Diversity, several Antennas with different Polarizations are used, which The most common method in mobile telecommunication station systems is... 

Cloud computing has brought about many changes in the new business arena. These changes do not only include technological changes, but have penetrated the business models of many companies. For example, in recent years, many companies have migrated from their former systems to cloud computing for reasons such as easy and low-cost access to high-performance hardware and software systems, and to avoid maintenance and repair costs and security costs.Due to the economic conditions in recent years and the high cost of electronic items, the criterion of purchase price has become much more important than in the past among people when buying such goods. Now in the Iranian electronics market,... 

Tipping points occur in diverse systems in various disciplines such as ecology, climate science, economy, sociology, and engineering. Critical thresholds in system parameters or state variables at which a tiny perturbation can lead to a qualitative change in the system exist in many subsystems in complex systems. These thresholds are called tipping points, and these subsystems are called tipping elements. Additionally, many systems with tipping points can be modeled as networks of coupled multistable subsystems. Domino-like tippings are called tipping cascades. Considering that these tipping cascades are primarily unprecedented, it is essential to study the dynamics and control of these... 

State estimation is a process in which values of unknown system state variables is obtained by considering measurements in such a system. State estimators’ output data are applied in control centers of power systems. Nowadays, using of phasor measurement unit (PMU) in WAMS (Wide Area Measurement Systems) and SCADA have significantly extended. PMUs increase the accuracy proportion compared with traditional measurement units, improve the power networks observability as well as detect and correct bad data. Since the possibility of removing the traditional measurement devices and replacing them with full PMU ones does not exist in the near future it is mandatory to find out an efficient and... 

Unsupervised neural spike sorting is a crucial tool in studying neural systems in the resolution of a neural cell. In extracellular recording from neural cells, the voltage of media is captured by the electrodes. The situation is possible that an electrode record activity of multiple neurons at the same time. The spike sorting goal is assigning each spike (extracellular recorded neural action potential) to a neural cell that generates it. Conventionally, more than one electrode is used to recording media voltages. The electrodes are placed in a small space as a single device called a multi-electrode array. After the spike sorting procedure, the occurrence time of activity of several cells is... 

The advent and advance of single-cell technologies have enabled us to measure the cell function and identity by using different assays and viewing it by different technologies. Nowadays, we are able to measure multiple feature vectors from same- single cells from multiple abstract molecular levels (genome, transcriptome, proteome, ...) simultaneously. Hence, the analysts can view the cell from different yet correlated angles and study their behaviours. Such progress in joint single-cell assessments plus the development and spread of more common single-cell assays - that measure one feature vector per cell - caused the growing need for computational tools to integrate these datasets in order... 

Triple Negative Breast Cancer (TNBC) is an invasive subtype of breast cancer. Finding prognostic biomarkers is helpful in choosing the appropriate treatment procedure for patients of this cancer. In recent years, the role of microRNAs in various biological processes, including cancer, has been identified, and their accessibility and stability have made these types of molecules an ideal biomarker. In the first phase of this study, with the aim of overcoming the limitations of previous studies, a new bioinformatics protocol has been proposed to investigate the prognostic miRNAs of triple negative breast cancer. First, using survival analysis, 56 prognostic miRNAs which had a significant...