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Studying the Effective Factors and Optimizing Enzyme (α-amylase) Separation in Multi-Enzyme Systems by Agarose Column Chromatography
, Ph.D. Dissertation Sharif University of Technology ; Roosta Azad, Reza (Supervisor) ; Soheila Yaghmaee (Supervisor) ; Yeganeh Sarkandi, Shahin (Co-Supervisor)
Abstract
In all types of chromatography for the extraction and purification of proteins, a fixed substrate is needed with which the mobile phase containing the proteins interact with them. This fixed bed should contain grains that have characteristics such as sphericity, high surface area, hydrophilicity, binding power to functional groups, chemical stability, low specific absorption, reusability and low cost. Commercially, the most commonly substrates in the ranges of the molecular weight of proteins are agarose and dextran. Agar was extracted from Gracilaria salicornia seaweed and removal of ionic groups was investigated by three methods. In washing with isopropanol, the ionic groups were reduced...