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chromatography
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The Synthesis and Characterization of Nano Zeolitic Molecular Sieve for Separation of 133Xe from 85Kr
, Ph.D. Dissertation Sharif University of Technology ; Kazemeini, Mohammad (Supervisor) ; Ahmadi, Javad (Supervisor)
Abstract
In this study the feasibility of xenon separation from xenon-krypton mixture was investigated by utilizing of the chromatographic column filled with nano and micron sized NaY zeolite. For this purpose the nano NaY with the yield of 100% related to Al2O3 and the average size of 98.6 nm was synthesized through the clear solution method with the mid-adding of NaOH. Furthermore, a novel method for granulation of nano zeolite was developed by utilizing sodium alginate as an external template. In this method the production of uniform spherical granules with the different sizes in the range of several milimiteres to 100 µm was easily performed. Moreover, the results showed that the adding of...
Separation and Purification of Industrial Proteins by Chromatography and Aqueous two Phase System
, M.Sc. Thesis Sharif University of Technology ; Vossoughi, Manouchehr (Supervisor) ; Seifkordi, Ali Akbar (Supervisor)
Abstract
Separation of biomolecules such as enzymes is the most important part of biotechnology process which 10-90 % of costs is for separation and purification. There are several methods for purification of bio materials and one of them is chromatography which use in enzyme purification but this method have several problem such as cost ,time consuming and scale up Because of this a suitable alternative demands for industrial separation. An alternative is aqueous two-phase systems (ATPS). In this project was studied about partial purification of alkaline protease produced by Bacillus licheniformis ATCC 21424 in aqueous two-phase system and comparison with chromatography method. an aqueous two-phase...
Development and Application of Multivariate Curve Resolution Techniques for the Analysis of Complex Chromatographic Data
, Ph.D. Dissertation Sharif University of Technology ; Jalali Heravi, Mahdi (Supervisor)
Abstract
In recent years, chromatographic techniques as one of the most important analytical techniques have been greatly developed from instrumentation and data volume point of views. On the other side, multivariate curve resolution (MCR) techniques as a branch of chemometrics have potential for the analysis of multivariate and huge data sets. In addition, MCR methods have been developed very fast in these years and their potential have been shown in the analysis of different types of chemical data. Thus, combination of sophisticated chromatographic techniques (e.g. gas chromatography-mass spectrometry (GC-MS) or comprehensive two-dimensional gas chromatography (GC×GC)) with MCR techniques can be...
Design and Simulation of Simulated Moving Beds
, M.Sc. Thesis Sharif University of Technology ; Farhadi, Fathollah (Supervisor)
Abstract
In this thesis design and simulation of SMB for four different mixture was done with Aspen chromatography package. Simulation for three mixtures of D-Psicose and D-Fructose, racemic mixture of Bupivacaine and Ibuprofen racemate were done with the aim of validation, based on experimental data of articles. The simulation results matched well with the experimental results in articles. The separation of p-xylene from mixed C8 aromatics and Para Diethyl benzene (as desorbent), was simulated for one of the p-xylene producing units and results were validated against the PFD. The influence of various parameters on the purity and recovery of p-xylene were investigated. The studies showed high...
Development and Application of Pattern Recognition Methods Combined with FT-IR Spectroscopy and HPTLC Techniques to Detect the Type and Amount of Fraud in Iranian Saffron Sample
, M.Sc. Thesis Sharif University of Technology ; Parastar, Hadi (Supervisor) ; Daraei, Bahram (Supervisor) ; Amir Ahmadi, Maryam (Co-Supervisor)
Abstract
Nowadays, with increasing concerns about health of food and its influence on human health,food authenticity has become a vital issue and a major challenge for consumers and regulatory agencies. It's especially remarkable about food with high nutritional and economical value. On the other hand, solving these challenges necessitates the use of fast and reliable techniques.Among the various foods, saffron is the main candidate for food adulteration due to its low production and high economic value and from this viewpoint it's in fourth place. Since the scope of fraud in this food is vast and also Iran is the major producer of saffron in the world,the present project has highlighted the...