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Synthesis and Stability Evaluation of Single Enzyme Nanoparticles for Enzymatic Based Processes
, Ph.D. Dissertation Sharif University of Technology ; Vossoughi, Manoochehr (Supervisor) ; Shahrokhian, Saeed (Co-Advisor) ; Alemzadeh, Iran (Co-Advisor)
Abstract
Enzymes, the nanometric biocatalysts that play a key role in biochemical reactions of living cells and bio-organisms, offer various applications including biosensing, bioremediation and chemical synthesis. A simple bio-conjugation procedure to surround a single horseradish peroxidase (HRP) enzyme molecule with dendritic polyester macromolecules (Polyester-32-hydroxyl-1-carboxyl bis-MPA Dendron, Generation 5) was proposed in this project. The characterization of resultant nanoparticles entitled HRP dendrozyme, was performed by TEM, DLS, GPC and FTIR spectroscopy. The results showed that HRP nanoparticles were spherical in shape and have an average size of 14 nm in diameter. Furthermore,...