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electroporation
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The Effect of Electroporation on Ultrasund Signals
, M.Sc. Thesis Sharif University of Technology ; Zahedi, Edmond (Supervisor)
Abstract
In this thesis, a cellular model of electroporation process and its detection by ultrasound signals is proposed. Electroporation is permeabilization of the cell membrane, caused by an external electric field. The resulted pores open cell’s membrane to large or hydrophilic molecules. The process of Electroporation has been used in Gene therapy and treatment of solid tumors. In this project, first we made a phantom of electroporated tissue and a phantom of normal tissue and then we simulated their responses to a linear array ultrasound transducer. By comparision of received signals from phantoms the probable effect of electroporation on ultrasound RF signals revealed. Results show up to 5 %...
Evaluation of Plasmid Transmission in Bacillus Subtilis by Cell Wall Modification
, M.Sc. Thesis Sharif University of Technology ; Roosta Azad, Reza (Supervisor) ; Aghamollaei, Hossein (Supervisor)
Abstract
Today, almost all the country's needs in the field of industrial enzymes are met through imports. In this regard, much attention has been paid to the industrial production of various enzymes in the country. The production of recombinant proteins through strain engineering is one of the most important ways to meet this demand due to its many benefits. One of the most important microorganisms that is widely used in the enzyme industry is Bacillus subtilis. Due to its thick peptidoglycan wall, this gram-positive bacterium hardly accepts engineered carriers for genetic modification as hosts. The aim of this study was to evaluate the permeability of the wall and cell membrane of Bacillus subtilis...