Sharif Digital Repository

In the first part of this research, we reported a liquid sensor array for detection and classification of biological thiols, based on aggregation of gold nanoparticles (AuNPs) with different coatings as sensor elements. Thiol mediated aggregation of AuNPs was visualized via UV–vis spectra. The spectral changes, as a consequence of aggregation of AuNPs, made a unique pattern of spectra for each analyte and allowed for the selective detection and discrimination of the biological molecules. HCA and PCA analysis demonstrated the discrimination of various thiols of different concentrations (i.e. 10–800 mmol L-1 for cysteine, 200–700 mmol L-1 for both glutathione and glutathione disulfide).... 

In the first part of this research, we introduce a novel chemiluminescence (CL) sensor array for discrimination of biothiols (e.g., cysteine, glutathione and glutathione disulfide). The proposed CL sensor array is based on the CL efficiencies of four types of enhanced nanoparticle-based CL systems. The intensity of CL was altered to varying degrees upon interaction with biothiols, producing unique CL response patterns. These distinct CL response patterns were collected as “fingerprints” and were then identified through chemometric methods, including linear discriminant analysis (LDA) and hierarchical cluster analysis (HCA). The developed array was able to successfully differentiate between...