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estrogens
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Liquid-liquid-liquid microextraction and high performance liquid Chromatography for Seperation and Determination of Estrogens in Aquatic Media
,
M.Sc. Thesis
Sharif University of Technology
;
Bagheri, Habib
(Supervisor)
Abstract
Liquid-liquid-liquid microextraction (LLLME) is a new miniaturized liquid extraction to preconcentrate and purify for ionizable analytes. In LLLME firstly analytes extracted in an organic phase, and subsequently back-extracted into a second aqueous phase. Main advantages of LLME are their speed, simplicity, negligible volume of solvents, analytical precision, short sample preparation and low cost. This technique was shown to be an efficient preconcentration method for the extraction of estrone and estriol from biological samples. A basic microdrop in the tip of microsyringe was fixed in the organic layer over the sample solution. After extraction for a preset time, the microdrop was...
Electrospun Functionalized Silica / Polyvinyl Alcohol Nanofibers for On-Line μ-Solid Phase Extraction of Estrone and Estradiol in Biological Samples
, M.Sc. Thesis Sharif University of Technology ; Bagheri, Habib (Supervisor)
Abstract
A method for determination of estrogens in biological samples was developed. This method was based on high performance liquid chromatography using online micro-solid phase extraction with 25 mg sorbent synthesized by electrospining of silica/PVA nanofibers. Poly (vinyl alcohol) (PVA) is an easily electrospun polymer with unique properties such as biocompatibility, processability, and hydrophilicity. Silica is biocompatible, thermally stable, and comparatively inert. Incorporation of silica with PVA produces hybrids with enhanced thermal and chemical stability in sample media and elution solvents without relinquishing the biocompatibility of PVA. The sufficient numbers of disc sheets were cut...