Loading...
Search for:
exosomes
0.078 seconds
Design, Fabrication and Evaluation of Polymeric Microcapsules Containing Cells to Use in Cell Therapy in Heart
, M.Sc. Thesis Sharif University of Technology ; Mashayekhan, Shohreh (Supervisor) ; Sharifi, Ali Mohammad (Supervisor) ; Khanmohammadi, Mehdi (Co-Supervisor)
Abstract
Because of the inability of conventional methods to regenerate the infarcted part of the heart, regenerative medicine based on using scaffolds and hydrogels incorporation with cells and biological factors emerges as a promising approach. Micro-structures, especially hollow microcapsules created by the microfluidic system, has received a great deal of attention due to their inspired biomimetic structures, providing complete coverage cells, and preventing an immune response, consequently. This study aimed to use a microfluidic system to fabricate hollow microcapsules and investigate the behavior of rat cardiomyoblast cells (H9C2) along with exosomes within these structures. These structures'...
Micro-Nanofluidic Isolation of Circulating Exosomes from Blood Plasma
, M.Sc. Thesis Sharif University of Technology ; Taghipoor, Mojtaba (Supervisor) ; saeedi, Mohammad saeed (Supervisor)
Abstract
Exosomes are a type of extracellular vesicles which are secreted by cells and could be found in all of human biofluids including blood. The size of exosomes is in the range of 30 – 150 nm. And they have a spherical structure with a phospholipid membrane. Exosomes are very important in academic research for the purpose of diagnostic and therapeutic practices because of their protein and nucleic acid contents.In this study a technological system was developed for isolation of circulating exosomes of human whole blood. This system consisted of a collection of microfluidic chips and protocols in order to extract a purified sample of exosomes from whole blood. First, a chip based on centrifugal...