Sharif Digital Repository

Folded protein experience several conformational substates. Each substate reperesents a minimum in the free energy surface of the system. Since Myoglobin is the smallest protein that can reversibly bind to gas molecules and, it can be used as prototype for more complex systems.IR pecrtum of CO inside of myolgobin shows that distinct conformational substates can occur. It was shown that Histidine residue inside heme pocket plays an essential rolle in structural properties of substates. In this study, we used molecular dynamice simulation together with umbrella sampling method to obtain free energy profile of MbCO system. The configuration of distal Histidine (His64), CO and Heme is studied... 

This thesis discusses the work on the production of a rhamnolipid-type biosurfactant by Pseudomonas aeruginosa MR01, its structural characterization and environmental applications. First, results from analytical approaches for screening of biosurfactant producers suggested the oil spreading method as the fastest, simplest and most consistent analytical method. The second part of the thesis describes the properties of the most efficient biosurfactant-producing bacteria isolated from oil fields in south of Iran and according to it’s biochemical characteristics and partial sequenced 16S rRNA gene affiliated with Pseudomonas aeruginosa. Time course study indicated that the maximum biosurfactant... 

There are different classes of event-driven softwares. These softwares include Graphical user interface, web application or embedded software. In these kinds of softwares, each user's action while using application is known as "event". The application responds to the action through changing behaviour and then waiting for another event. Testing software is the best way for checking the quality assurance of event-driven softwares. However, there are some problems as cause of creating lots of event sequences in testing these kinds of softwares. Therefore, many solutions are introduced for these problems. The goal of this report, is presenting new testing procedure for event-driven web...